ID   T9025_TANPA             Reviewed;         493 AA.
AC   X2EVQ5;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   11-JUN-2014, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Cytochrome P450 Tp9025 {ECO:0000312|EMBL:AHM24032.1};
DE            Short=Tp9025 {ECO:0000303|PubMed:24704560};
OS   Tanacetum parthenium (Feverfew) (Matricaria parthenium).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC   Anthemidinae; Tanacetum.
OX   NCBI_TaxID=127999;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX   PubMed=24704560; DOI=10.1016/j.ymben.2014.03.005;
RA   Liu Q., Manzano D., Tanic N., Pesic M., Bankovic J., Pateraki I.,
RA   Ricard L., Ferrer A., de Vos R., van de Krol S., Bouwmeester H.;
RT   "Elucidation and in planta reconstitution of the parthenolide biosynthetic
RT   pathway.";
RL   Metab. Eng. 23C:145-153(2014).
RN   [2]
RP   PATHWAY, AND REVIEW.
RX   PubMed=30468448; DOI=10.1039/c8np00077h;
RA   Liu Y., Jing S.-X., Luo S.-H., Li S.-H.;
RT   "Non-volatile natural products in plant glandular trichomes: chemistry,
RT   biological activities and biosynthesis.";
RL   Nat. Prod. Rep. 36:626-665(2019).
CC   -!- FUNCTION: Probably involved in the biosynthesis of germacrene-derived
CC       sesquiterpene lactones. {ECO:0000303|PubMed:24704560}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000250|UniProtKB:P04798};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000303|PubMed:30468448}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass type II
CC       membrane protein {ECO:0000255}.
CC   -!- DEVELOPMENTAL STAGE: During ovary development, accumulates until the
CC       stage 3 and fades out progressively to disappear at stage 6.
CC       {ECO:0000269|PubMed:24704560}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; KC954154; AHM24032.1; -; mRNA.
DR   AlphaFoldDB; X2EVQ5; -.
DR   SMR; X2EVQ5; -.
DR   UniPathway; UPA00213; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0051762; P:sesquiterpene biosynthetic process; IEA:UniProt.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Signal-anchor; Transmembrane; Transmembrane helix.
FT   CHAIN           1..493
FT                   /note="Cytochrome P450 Tp9025"
FT                   /id="PRO_0000448400"
FT   TRANSMEM        1..21
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   BINDING         433
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
FT   CARBOHYD        209
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        411
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   493 AA;  55417 MW;  A63713398548C6D1 CRC64;
     MALYIIFLLI ASSFILFSFI FSQKPKGKLP PGPPKLPIIG NIHQTVGGLP HYVFRDLAKK
     YGPIMHLQLG QVSIMVVSSP RLAEEVLKTN DIVVANRPYS LVGDIVLYGS SDVAFGSYGD
     YWRQMKKIMT VEVLSAKKVA AISGTRQQEV NNLMEYIRST CGKPFHVREH VMQRNNNIIC
     KSLFGDNCKQ QHVLIESLDE MMKLSTGFNV SDLFPNWGFL PVISGYKSTL TRIHKNIDSI
     LSEIFEERKI KRQKGGASDE DMLDVLLNIK ERGGLEFPVA DNNIKAIFVN MFVGGTDTSV
     ATIEWAMTEL MRNPKVMSKA QAEVRQLFKG KNTILDKDLQ DLVYLKYIIK ETLRLHPVVP
     ILLPRECRGP CKIGGYDIPM NTVLFVNAFA CSTDPEYWDD AESFKPERFE NSSLDLNGRA
     CEYLPFGAGR RMCPGITFGM SVVEIILAQL LYYFNWELPN GLSPNDIDLT PNFGAVADKK
     VPLQIVPTLN SLK