TA2R4_MOUSE
ID TA2R4_MOUSE Reviewed; 297 AA.
AC Q9JKT3; Q53Z44;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Taste receptor type 2 member 4;
DE Short=T2R4;
DE AltName: Full=Taste receptor type 2 member 8;
DE Short=T2R8;
GN Name=Tas2r4; Synonyms=Tas2r108, Tas2r8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TOPOLOGY.
RC STRAIN=129/SvJ;
RX PubMed=10761934; DOI=10.1016/s0092-8674(00)80705-9;
RA Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P.,
RA Zuker C.S.;
RT "A novel family of mammalian taste receptors.";
RL Cell 100:693-702(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=15886333; DOI=10.1152/physiolgenomics.00030.2005;
RA Wu S.V., Chen M.C., Rozengurt E.;
RT "Genomic organization, expression, and function of bitter taste receptors
RT (T2R) in mouse and rat.";
RL Physiol. Genomics 22:139-149(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C3Heb/FeJ, and SWR/J;
RX PubMed=14627538; DOI=10.1093/chemse/bjg062;
RA Nelson T.M., Munger S.D., Boughter J.D. Jr.;
RT "Taste sensitivities to PROP and PTC vary independently in mice.";
RL Chem. Senses 28:695-704(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP CHARACTERIZATION.
RX PubMed=10761935; DOI=10.1016/s0092-8674(00)80706-0;
RA Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W.,
RA Zuker C.S., Ryba N.J.P.;
RT "T2Rs function as bitter taste receptors.";
RL Cell 100:703-711(2000).
RN [6]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [7]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [8]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Gustducin-coupled receptor for denatonium and N(6)-propyl-2-
CC thiouracil implicated in the perception of bitter compounds in the oral
CC cavity and the gastrointestinal tract. Signals through PLCB2 and the
CC calcium-regulated cation channel TRPM5. In airway epithelial cells,
CC binding of denatonium increases the intracellular calcium ion
CC concentration and stimulates ciliary beat frequency (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Cell
CC projection, cilium membrane. Note=In airway epithelial cells, localizes
CC to motile cilia. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC tongue and palate epithelium and exclusively in gustducin-positive
CC cells. Expressed in 15% taste bud cells in circumvallate and foliate
CC papillae but only in 2% in fungiform papillae.
CC -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single
CC taste receptor cell.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
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DR EMBL; AF227148; AAF43921.1; -; Genomic_DNA.
DR EMBL; AY916507; AAX99130.1; -; mRNA.
DR EMBL; AY364474; AAQ93083.1; -; Genomic_DNA.
DR EMBL; AY364475; AAQ93084.1; -; Genomic_DNA.
DR EMBL; BC116952; AAI16953.1; -; mRNA.
DR CCDS; CCDS20032.1; -.
DR RefSeq; NP_065248.1; NM_020502.1.
DR AlphaFoldDB; Q9JKT3; -.
DR SMR; Q9JKT3; -.
DR BioGRID; 208223; 1.
DR STRING; 10090.ENSMUSP00000044089; -.
DR GlyGen; Q9JKT3; 1 site.
DR PaxDb; Q9JKT3; -.
DR PRIDE; Q9JKT3; -.
DR Antibodypedia; 32508; 38 antibodies from 17 providers.
DR DNASU; 57253; -.
DR Ensembl; ENSMUST00000038750; ENSMUSP00000044089; ENSMUSG00000037140.
DR GeneID; 57253; -.
DR KEGG; mmu:57253; -.
DR UCSC; uc009bmw.1; mouse.
DR CTD; 57253; -.
DR MGI; MGI:2681210; Tas2r108.
DR VEuPathDB; HostDB:ENSMUSG00000037140; -.
DR eggNOG; ENOG502S2SI; Eukaryota.
DR GeneTree; ENSGT00960000186638; -.
DR HOGENOM; CLU_072337_3_0_1; -.
DR InParanoid; Q9JKT3; -.
DR OMA; GAMKLMI; -.
DR OrthoDB; 1010573at2759; -.
DR PhylomeDB; Q9JKT3; -.
DR TreeFam; TF335891; -.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-MMU-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR BioGRID-ORCS; 57253; 3 hits in 73 CRISPR screens.
DR PRO; PR:Q9JKT3; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9JKT3; protein.
DR Bgee; ENSMUSG00000037140; Expressed in animal zygote.
DR Genevisible; Q9JKT3; MM.
DR GO; GO:0060170; C:ciliary membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0033038; F:bitter taste receptor activity; ISO:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0008527; F:taste receptor activity; TAS:UniProtKB.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; ISA:MGI.
DR GO; GO:0007585; P:respiratory gaseous exchange by respiratory system; IMP:MGI.
DR InterPro; IPR007960; TAS2R.
DR InterPro; IPR030055; TAS2R4.
DR PANTHER; PTHR11394:SF55; PTHR11394:SF55; 1.
DR Pfam; PF05296; TAS2R; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cell projection; Cilium; G-protein coupled receptor;
KW Glycoprotein; Membrane; Receptor; Reference proteome; Sensory transduction;
KW Taste; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..297
FT /note="Taste receptor type 2 member 4"
FT /id="PRO_0000082210"
FT TOPO_DOM 1..11
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..80
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..171
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..260
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 282..297
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 297 AA; 34202 MW; EC2C1EAE06571F80 CRC64;
MLWELYVFVF AASVFLNFVG IIANLFIIVI IIKTWVNSRR IASPDRILFS LAITRFLTLG
LFLLNSVYIA TNTGRSVYFS TFFLLCWKFL DANSLWLVTI LNSLYCVKIT NFQHPVFLLL
KRTISMKTTS LLLACLLISA LTTLLYYMLS QISRFPEHII GRNDTSFDLS DGILTLVASL
VLNSLLQFML NVTFASLLIH SLRRHIQKMQ RNRTSFWNPQ TEAHMGAMRL MICFLVLYIP
YSIATLLYLP SYMRKNLRAQ AICMIITAAY PPGHSVLLII THHKLKAKAK KIFCFYK
