TA2R4_RAT
ID TA2R4_RAT Reviewed; 304 AA.
AC Q67ET0;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Taste receptor type 2 member 4;
DE Short=T2R4;
DE AltName: Full=T2R16;
DE AltName: Full=Taste receptor type 2 member 20;
DE Short=T2R20;
GN Name=Tas2r4 {ECO:0000250|UniProtKB:Q9JKT3};
GN Synonyms=T2r16 {ECO:0000312|RGD:1561194}, T2r20;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAX99133.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley {ECO:0000269|PubMed:15886333};
RX PubMed=15886333; DOI=10.1152/physiolgenomics.00030.2005;
RA Wu S.V., Chen M.C., Rozengurt E.;
RT "Genomic organization, expression, and function of bitter taste receptors
RT (T2R) in mouse and rat.";
RL Physiol. Genomics 22:139-149(2005).
RN [2] {ECO:0000312|EMBL:AAR13349.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Sprague-Dawley {ECO:0000312|EMBL:AAR13349.1};
RA Conte C., Ebeling M., Marcuz A., Andres-Barquin P.J.;
RT "Identification of new putative rat taste receptors belonging to the T2R
RT family.";
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Gustducin-coupled receptor implicated in the perception of
CC bitter compounds in the oral cavity and the gastrointestinal tract.
CC Signals through PLCB2 and the calcium-regulated cation channel TRPM5
CC (By similarity). In airway epithelial cells, binding of denatonium
CC increases the intracellular calcium ion concentration and stimulates
CC ciliary beat frequency (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Cell projection, cilium membrane. Note=In airway
CC epithelial cells, localizes to motile cilia. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in tongue, stomach and duodenum.
CC {ECO:0000269|PubMed:15886333}.
CC -!- MISCELLANEOUS: Several bitter taste receptors are expressed in a single
CC taste receptor cell. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000255}.
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DR EMBL; AY916510; AAX99133.1; -; mRNA.
DR EMBL; AY362740; AAR13349.1; -; Genomic_DNA.
DR RefSeq; NP_001019857.1; NM_001024686.1.
DR AlphaFoldDB; Q67ET0; -.
DR SMR; Q67ET0; -.
DR STRING; 10116.ENSRNOP00000016232; -.
DR GlyGen; Q67ET0; 1 site.
DR PaxDb; Q67ET0; -.
DR Ensembl; ENSRNOT00000016232; ENSRNOP00000016232; ENSRNOG00000012195.
DR GeneID; 554302; -.
DR KEGG; rno:554302; -.
DR UCSC; RGD:1561194; rat.
DR CTD; 57253; -.
DR RGD; 1561194; T2R16.
DR eggNOG; ENOG502S2SI; Eukaryota.
DR GeneTree; ENSGT00960000186638; -.
DR HOGENOM; CLU_072337_1_0_1; -.
DR InParanoid; Q67ET0; -.
DR OMA; GAMKLMI; -.
DR OrthoDB; 1010573at2759; -.
DR PhylomeDB; Q67ET0; -.
DR TreeFam; TF335891; -.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR Reactome; R-RNO-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-RNO-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR PRO; PR:Q67ET0; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000012195; Expressed in cerebellum.
DR GO; GO:0060170; C:ciliary membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0033038; F:bitter taste receptor activity; ISO:RGD.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; ISO:RGD.
DR GO; GO:0007585; P:respiratory gaseous exchange by respiratory system; ISO:RGD.
DR InterPro; IPR007960; TAS2R.
DR InterPro; IPR030055; TAS2R4.
DR PANTHER; PTHR11394:SF55; PTHR11394:SF55; 1.
DR Pfam; PF05296; TAS2R; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cell projection; Cilium; G-protein coupled receptor;
KW Glycoprotein; Membrane; Receptor; Reference proteome; Sensory transduction;
KW Taste; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..304
FT /note="Taste receptor type 2 member 4"
FT /id="PRO_0000248472"
FT TOPO_DOM 1..10
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..80
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..171
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..260
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 282..304
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 304 AA; 34811 MW; E8C0CF90FA1930C7 CRC64;
MLWELYAFVF AASVVFNFVG IVANLFIIVI ISKTWVKSHK ISSSDKILFS LAITRFLTLG
LFLLNTVYIA TNTGRSVYFS TFFLLCWKFL DSNSLWLVTF LNCLYCVKIT HFQHPVFLLL
KRTVSMKTTS LLLACLLISA FTTLLYFVLT QISRFPEHII GRNDTLFDVS DGILTLAASL
ILSSLLQFLL NVTFASLLIH SLRRHVQKMQ RNRSSFWNPQ TEAHVGAMRL MICFLVLYIP
YSIAALLYFP SYMRKNLRAQ AACMIITAAY PPGHSILLII THHKLKAKAK KICCFYKLRD
FVSN
