TA2R5_HUMAN
ID TA2R5_HUMAN Reviewed; 299 AA.
AC Q9NYW4; Q645W0; Q75MV7;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Taste receptor type 2 member 5;
DE Short=T2R5;
GN Name=TAS2R5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TOPOLOGY.
RX PubMed=10761934; DOI=10.1016/s0092-8674(00)80705-9;
RA Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P.,
RA Zuker C.S.;
RT "A novel family of mammalian taste receptors.";
RL Cell 100:693-702(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=15496549; DOI=10.1093/molbev/msi027;
RA Fischer A., Gilad Y., Man O., Paeaebo S.;
RT "Evolution of bitter taste receptors in humans and apes.";
RL Mol. Biol. Evol. 22:432-436(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP CHARACTERIZATION.
RX PubMed=10761935; DOI=10.1016/s0092-8674(00)80706-0;
RA Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W.,
RA Zuker C.S., Ryba N.J.P.;
RT "T2Rs function as bitter taste receptors.";
RL Cell 100:703-711(2000).
RN [6]
RP REVIEW.
RX PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA Montmayeur J.-P., Matsunami H.;
RT "Receptors for bitter and sweet taste.";
RL Curr. Opin. Neurobiol. 12:366-371(2002).
RN [7]
RP REVIEW.
RX PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA Margolskee R.F.;
RT "Molecular mechanisms of bitter and sweet taste transduction.";
RL J. Biol. Chem. 277:1-4(2002).
RN [8]
RP REVIEW.
RX PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA Zuker C.S., Ryba N.J.;
RT "Coding of sweet, bitter, and umami tastes: different receptor cells
RT sharing similar signaling pathways.";
RL Cell 112:293-301(2003).
CC -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC and is gustducin-linked. May play a role in sensing the chemical
CC composition of the gastrointestinal content. The activity of this
CC receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC and lead to the gating of TRPM5.
CC -!- INTERACTION:
CC Q9NYW4; P13236: CCL4; NbExp=3; IntAct=EBI-17933167, EBI-2873970;
CC Q9NYW4; Q5QGT7: RTP2; NbExp=3; IntAct=EBI-17933167, EBI-10244780;
CC Q9NYW4; O95562: SFT2D2; NbExp=3; IntAct=EBI-17933167, EBI-4402330;
CC Q9NYW4; Q9BRI3: SLC30A2; NbExp=3; IntAct=EBI-17933167, EBI-8644112;
CC Q9NYW4; Q14656: TMEM187; NbExp=3; IntAct=EBI-17933167, EBI-13046724;
CC Q9NYW4; O95183: VAMP5; NbExp=3; IntAct=EBI-17933167, EBI-10191195;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC tongue and palate epithelium and exclusively in gustducin-positive
CC cells.
CC -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC bitter compounds; individual taste cells can discriminate among bitter
CC stimuli.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF227132; AAF43905.1; -; Genomic_DNA.
DR EMBL; AY724966; AAU21159.1; -; Genomic_DNA.
DR EMBL; AC004979; AAS02040.1; -; Genomic_DNA.
DR EMBL; BC069509; AAH69509.1; -; mRNA.
DR EMBL; BC101586; AAI01587.1; -; mRNA.
DR EMBL; BC101588; AAI01589.1; -; mRNA.
DR CCDS; CCDS5869.1; -.
DR RefSeq; NP_061853.1; NM_018980.2.
DR AlphaFoldDB; Q9NYW4; -.
DR SMR; Q9NYW4; -.
DR BioGRID; 119946; 7.
DR IntAct; Q9NYW4; 6.
DR STRING; 9606.ENSP00000247883; -.
DR ChEMBL; CHEMBL4523473; -.
DR GuidetoPHARMACOLOGY; 662; -.
DR GlyGen; Q9NYW4; 1 site.
DR iPTMnet; Q9NYW4; -.
DR PhosphoSitePlus; Q9NYW4; -.
DR BioMuta; TAS2R5; -.
DR DMDM; 29839664; -.
DR PaxDb; Q9NYW4; -.
DR PeptideAtlas; Q9NYW4; -.
DR PRIDE; Q9NYW4; -.
DR Antibodypedia; 32509; 75 antibodies from 17 providers.
DR DNASU; 54429; -.
DR Ensembl; ENST00000247883.5; ENSP00000247883.4; ENSG00000127366.5.
DR GeneID; 54429; -.
DR KEGG; hsa:54429; -.
DR MANE-Select; ENST00000247883.5; ENSP00000247883.4; NM_018980.3; NP_061853.1.
DR UCSC; uc003vwr.2; human.
DR CTD; 54429; -.
DR DisGeNET; 54429; -.
DR GeneCards; TAS2R5; -.
DR HGNC; HGNC:14912; TAS2R5.
DR HPA; ENSG00000127366; Low tissue specificity.
DR MIM; 605062; gene.
DR neXtProt; NX_Q9NYW4; -.
DR OpenTargets; ENSG00000127366; -.
DR PharmGKB; PA37924; -.
DR VEuPathDB; HostDB:ENSG00000127366; -.
DR eggNOG; ENOG502S2SI; Eukaryota.
DR GeneTree; ENSGT00960000186638; -.
DR HOGENOM; CLU_072337_1_1_1; -.
DR InParanoid; Q9NYW4; -.
DR OMA; CRFLLQW; -.
DR OrthoDB; 1010573at2759; -.
DR PhylomeDB; Q9NYW4; -.
DR TreeFam; TF335891; -.
DR PathwayCommons; Q9NYW4; -.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR SignaLink; Q9NYW4; -.
DR BioGRID-ORCS; 54429; 9 hits in 1073 CRISPR screens.
DR GeneWiki; TAS2R5; -.
DR GenomeRNAi; 54429; -.
DR Pharos; Q9NYW4; Tchem.
DR PRO; PR:Q9NYW4; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9NYW4; protein.
DR Bgee; ENSG00000127366; Expressed in right uterine tube and 94 other tissues.
DR ExpressionAtlas; Q9NYW4; baseline and differential.
DR Genevisible; Q9NYW4; HS.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0008527; F:taste receptor activity; TAS:UniProtKB.
DR GO; GO:0007635; P:chemosensory behavior; TAS:ProtInc.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IDA:UniProtKB.
DR InterPro; IPR007960; TAS2R.
DR Pfam; PF05296; TAS2R; 1.
PE 1: Evidence at protein level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..299
FT /note="Taste receptor type 2 member 5"
FT /id="PRO_0000082212"
FT TOPO_DOM 1
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 2..22
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 23..45
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..82
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..127
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..175
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..223
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 245..253
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 275..299
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 155
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 20
FT /note="G -> S (in dbSNP:rs2234013)"
FT /id="VAR_053343"
FT VARIANT 26
FT /note="S -> I (in dbSNP:rs2227264)"
FT /id="VAR_020203"
FT VARIANT 113
FT /note="P -> L (in dbSNP:rs2234014)"
FT /id="VAR_034537"
FT VARIANT 167
FT /note="Y -> C (in dbSNP:rs34529840)"
FT /id="VAR_034538"
FT VARIANT 213
FT /note="R -> Q (in dbSNP:rs2234015)"
FT /id="VAR_024184"
FT VARIANT 294
FT /note="R -> L (in dbSNP:rs2234016)"
FT /id="VAR_053344"
SQ SEQUENCE 299 AA; 34505 MW; F85064A07E9018C7 CRC64;
MLSAGLGLLM LVAVVEFLIG LIGNGSLVVW SFREWIRKFN WSSYNLIILG LAGCRFLLQW
LIILDLSLFP LFQSSRWLRY LSIFWVLVSQ ASLWFATFLS VFYCKKITTF DRPAYLWLKQ
RAYNLSLWCL LGYFIINLLL TVQIGLTFYH PPQGNSSIRY PFESWQYLYA FQLNSGSYLP
LVVFLVSSGM LIVSLYTHHK KMKVHSAGRR DVRAKAHITA LKSLGCFLLL HLVYIMASPF
SITSKTYPPD LTSVFIWETL MAAYPSLHSL ILIMGIPRVK QTCQKILWKT VCARRCWGP
