ID   TA2R8_HUMAN             Reviewed;         309 AA.
AC   Q9NYW2; Q4KN29; Q645Y2;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 159.
DE   RecName: Full=Taste receptor type 2 member 8;
DE            Short=T2R8;
DE   AltName: Full=Taste receptor family B member 5;
DE            Short=TRB5;
GN   Name=TAS2R8;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TOPOLOGY.
RX   PubMed=10761934; DOI=10.1016/s0092-8674(00)80705-9;
RA   Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P.,
RA   Zuker C.S.;
RT   "A novel family of mammalian taste receptors.";
RL   Cell 100:693-702(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT VAL-308.
RX   PubMed=15496549; DOI=10.1093/molbev/msi027;
RA   Fischer A., Gilad Y., Man O., Paeaebo S.;
RT   "Evolution of bitter taste receptors in humans and apes.";
RL   Mol. Biol. Evol. 22:432-436(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=10761935; DOI=10.1016/s0092-8674(00)80706-0;
RA   Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W.,
RA   Zuker C.S., Ryba N.J.P.;
RT   "T2Rs function as bitter taste receptors.";
RL   Cell 100:703-711(2000).
RN   [5]
RP   REVIEW.
RX   PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA   Montmayeur J.-P., Matsunami H.;
RT   "Receptors for bitter and sweet taste.";
RL   Curr. Opin. Neurobiol. 12:366-371(2002).
RN   [6]
RP   REVIEW.
RX   PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA   Margolskee R.F.;
RT   "Molecular mechanisms of bitter and sweet taste transduction.";
RL   J. Biol. Chem. 277:1-4(2002).
RN   [7]
RP   REVIEW.
RX   PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA   Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA   Zuker C.S., Ryba N.J.;
RT   "Coding of sweet, bitter, and umami tastes: different receptor cells
RT   sharing similar signaling pathways.";
RL   Cell 112:293-301(2003).
CC   -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC       and is gustducin-linked. May play a role in sensing the chemical
CC       composition of the gastrointestinal content. The activity of this
CC       receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC       and lead to the gating of TRPM5.
CC   -!- INTERACTION:
CC       Q9NYW2; Q86UT5-3: PDZD3; NbExp=3; IntAct=EBI-12092809, EBI-12092811;
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC       tongue and palate epithelium and exclusively in gustducin-positive
CC       cells.
CC   -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC       bitter compounds; individual taste cells can discriminate among bitter
CC       stimuli.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC       {ECO:0000305}.
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DR   EMBL; AF227134; AAF43907.1; -; Genomic_DNA.
DR   EMBL; AY724935; AAU21137.1; -; Genomic_DNA.
DR   EMBL; BC069084; AAH69084.1; -; mRNA.
DR   EMBL; BC069551; AAH69551.1; -; mRNA.
DR   EMBL; BC096701; AAH96701.1; -; mRNA.
DR   EMBL; BC096735; AAH96735.1; -; mRNA.
DR   EMBL; BC098353; AAH98353.1; -; mRNA.
DR   CCDS; CCDS8632.1; -.
DR   RefSeq; NP_076407.1; NM_023918.1.
DR   AlphaFoldDB; Q9NYW2; -.
DR   SMR; Q9NYW2; -.
DR   BioGRID; 119146; 4.
DR   IntAct; Q9NYW2; 2.
DR   STRING; 9606.ENSP00000240615; -.
DR   BindingDB; Q9NYW2; -.
DR   ChEMBL; CHEMBL3988599; -.
DR   GlyGen; Q9NYW2; 1 site.
DR   iPTMnet; Q9NYW2; -.
DR   PhosphoSitePlus; Q9NYW2; -.
DR   BioMuta; TAS2R8; -.
DR   DMDM; 29839662; -.
DR   PaxDb; Q9NYW2; -.
DR   PeptideAtlas; Q9NYW2; -.
DR   PRIDE; Q9NYW2; -.
DR   Antibodypedia; 23396; 42 antibodies from 15 providers.
DR   DNASU; 50836; -.
DR   Ensembl; ENST00000240615.3; ENSP00000240615.2; ENSG00000121314.3.
DR   Ensembl; ENST00000572823.1; ENSP00000461158.1; ENSG00000272712.1.
DR   Ensembl; ENST00000610444.2; ENSP00000484163.1; ENSG00000277316.2.
DR   GeneID; 50836; -.
DR   KEGG; hsa:50836; -.
DR   MANE-Select; ENST00000240615.3; ENSP00000240615.2; NM_023918.3; NP_076407.1.
DR   UCSC; uc010shh.3; human.
DR   CTD; 50836; -.
DR   GeneCards; TAS2R8; -.
DR   HGNC; HGNC:14915; TAS2R8.
DR   HPA; ENSG00000121314; Not detected.
DR   MIM; 604794; gene.
DR   neXtProt; NX_Q9NYW2; -.
DR   OpenTargets; ENSG00000121314; -.
DR   PharmGKB; PA37926; -.
DR   VEuPathDB; HostDB:ENSG00000121314; -.
DR   eggNOG; ENOG502SKRK; Eukaryota.
DR   GeneTree; ENSGT00960000186648; -.
DR   HOGENOM; CLU_072337_3_0_1; -.
DR   InParanoid; Q9NYW2; -.
DR   OMA; NWIDWIK; -.
DR   OrthoDB; 1010573at2759; -.
DR   PhylomeDB; Q9NYW2; -.
DR   TreeFam; TF335891; -.
DR   PathwayCommons; Q9NYW2; -.
DR   Reactome; R-HSA-418594; G alpha (i) signalling events.
DR   Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR   Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR   SignaLink; Q9NYW2; -.
DR   BioGRID-ORCS; 50836; 10 hits in 1066 CRISPR screens.
DR   GeneWiki; TAS2R8; -.
DR   GenomeRNAi; 50836; -.
DR   Pharos; Q9NYW2; Tchem.
DR   PRO; PR:Q9NYW2; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; Q9NYW2; protein.
DR   Bgee; ENSG00000121314; Expressed in cortical plate and 6 other tissues.
DR   Genevisible; Q9NYW2; HS.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR   GO; GO:0008527; F:taste receptor activity; TAS:UniProtKB.
DR   GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IDA:UniProtKB.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   InterPro; IPR007960; TAS2R.
DR   Pfam; PF05296; TAS2R; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE   1: Evidence at protein level;
KW   G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW   Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..309
FT                   /note="Taste receptor type 2 member 8"
FT                   /id="PRO_0000082227"
FT   TOPO_DOM        1..7
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        8..28
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        29..50
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        72..82
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        104..131
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..152
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        153..184
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        206..239
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        261..266
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        267..287
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        288..309
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        167
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VARIANT         308
FT                   /note="M -> V (in dbSNP:rs2537817)"
FT                   /evidence="ECO:0000269|PubMed:15496549"
FT                   /id="VAR_024186"
SQ   SEQUENCE   309 AA;  35877 MW;  5E3D94B726A52413 CRC64;
     MFSPADNIFI ILITGEFILG ILGNGYIALV NWIDWIKKKK ISTVDYILTN LVIARICLIS
     VMVVNGIVIV LNPDVYTKNK QQIVIFTFWT FANYLNMWIT TCLNVFYFLK IASSSHPLFL
     WLKWKIDMVV HWILLGCFAI SLLVSLIAAI VLSCDYRFHA IAKHKRNITE MFHVSKIPYF
     EPLTLFNLFA IVPFIVSLIS FFLLVRSLWR HTKQIKLYAT GSRDPSTEVH VRAIKTMTSF
     IFFFFLYYIS SILMTFSYLM TKYKLAVEFG EIAAILYPLG HSLILIVLNN KLRQTFVRML
     TCRKIACMI