TA34_TREPA
ID TA34_TREPA Reviewed; 204 AA.
AC P19478;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=34 kDa membrane antigen;
DE AltName: Full=Pathogen-specific membrane antigen;
DE Flags: Precursor;
GN Name=tpd; OrderedLocusNames=TP_0971;
OS Treponema pallidum (strain Nichols).
OC Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX NCBI_TaxID=243276;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=Nichols;
RX PubMed=2680970; DOI=10.1128/iai.57.11.3314-3323.1989;
RA Swancutt M.A., Riley B.S., Radolf J.D., Norgard M.V.;
RT "Molecular characterization of the pathogen-specific, 34-kilodalton
RT membrane immunogen of Treponema pallidum.";
RL Infect. Immun. 57:3314-3323(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nichols;
RX PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M., Utterback T.R.,
RA McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A.,
RA Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O.,
RA Venter J.C.;
RT "Complete genome sequence of Treponema pallidum, the syphilis spirochete.";
RL Science 281:375-388(1998).
RN [3]
RP MUTAGENESIS OF CYS-20, DIACYLGLYCEROL AT CYS-20, AND PALMITOYLATION AT
RP CYS-20.
RC STRAIN=Nichols;
RX PubMed=1894360; DOI=10.1128/iai.59.10.3536-3546.1991;
RA Schouls L.M., van der Heide H.G.J., van Embden J.D.A.;
RT "Characterization of the 35-kilodalton Treponema pallidum subsp. pallidum
RT recombinant lipoprotein TmpC and antibody response to lipidated and
RT nonlipidated T. pallidum antigens.";
RL Infect. Immun. 59:3536-3546(1991).
CC -!- FUNCTION: This antigen is a pathogen-specific membrane immunogen.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the UPF0423 family. {ECO:0000305}.
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DR EMBL; J04241; AAA27474.1; -; Genomic_DNA.
DR EMBL; AE000520; AAC65921.1; -; Genomic_DNA.
DR PIR; A40182; A40182.
DR RefSeq; WP_010882415.1; NC_021490.2.
DR PDB; 2O6C; X-ray; 1.70 A; A/B=18-204.
DR PDB; 2O6D; X-ray; 1.86 A; A/B=18-204.
DR PDB; 2O6E; X-ray; 1.90 A; A/B=18-204.
DR PDB; 2O6F; X-ray; 1.63 A; A/B=18-204.
DR PDB; 3PJL; X-ray; 1.70 A; A/B=21-204.
DR PDB; 3PJN; X-ray; 1.70 A; A/B=21-204.
DR PDBsum; 2O6C; -.
DR PDBsum; 2O6D; -.
DR PDBsum; 2O6E; -.
DR PDBsum; 2O6F; -.
DR PDBsum; 3PJL; -.
DR PDBsum; 3PJN; -.
DR AlphaFoldDB; P19478; -.
DR SMR; P19478; -.
DR IntAct; P19478; 1.
DR STRING; 243276.TPANIC_0971; -.
DR EnsemblBacteria; AAC65921; AAC65921; TP_0971.
DR GeneID; 57879480; -.
DR KEGG; tpa:TP_0971; -.
DR eggNOG; COG3470; Bacteria.
DR HOGENOM; CLU_100963_0_0_12; -.
DR OMA; GRFWTEP; -.
DR OrthoDB; 1456006at2; -.
DR EvolutionaryTrace; P19478; -.
DR Proteomes; UP000000811; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.40.2480; -; 1.
DR InterPro; IPR018470; Metal-bd_Tp34-typ.
DR InterPro; IPR038482; Tp34-type_sf.
DR Pfam; PF10634; Iron_transport; 1.
DR PIRSF; PIRSF017018; Tp34; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Direct protein sequencing; Lipoprotein;
KW Membrane; Palmitate; Reference proteome; Signal.
FT SIGNAL 1..19
FT CHAIN 20..204
FT /note="34 kDa membrane antigen"
FT /id="PRO_0000018201"
FT LIPID 20
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT ECO:0000269|PubMed:1894360"
FT LIPID 20
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305|PubMed:1894360"
FT MUTAGEN 20
FT /note="C->S: Loss of lipid incorporation."
FT /evidence="ECO:0000269|PubMed:1894360"
FT STRAND 50..54
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 61..69
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:2O6F"
FT TURN 83..85
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 87..96
FT /evidence="ECO:0007829|PDB:2O6F"
FT HELIX 98..103
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 115..122
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 134..138
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 141..148
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 154..164
FT /evidence="ECO:0007829|PDB:2O6F"
FT TURN 168..170
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 172..174
FT /evidence="ECO:0007829|PDB:2O6F"
FT TURN 177..179
FT /evidence="ECO:0007829|PDB:2O6F"
FT STRAND 190..199
FT /evidence="ECO:0007829|PDB:2O6F"
SQ SEQUENCE 204 AA; 22085 MW; 2ABD8A42965034D0 CRC64;
MKRVSLLGSA AIFALVFSAC GGGGEHQHGE EMMAAVPAPD AEGAAGFDEF PIGEDRDVGP
LHVGGVYFQP VEMHPAPGAQ PSKEEADCHI EADIHANEAG KDLGYGVGDF VPYLRVVAFL
QKHGSEKVQK VMFAPMNAGD GPHYGANVKF EEGLGTYKVR FEIAAPSHDE YSLHIDEQTG
VSGRFWSEPL VAEWDDFEWK GPQW
