TACHY_APIME
ID TACHY_APIME Reviewed; 339 AA.
AC Q868G6; Q86DQ5; Q86DQ6; Q86DQ7;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Tachykinins;
DE AltName: Full=AmTRP;
DE AltName: Full=Tachykinin-related peptide;
DE Contains:
DE RecName: Full=APTGHQEMQ-amide;
DE AltName: Full=AmTRP1;
DE AltName: Full=Tachykinin-related peptide 1;
DE Short=TRP1;
DE Contains:
DE RecName: Full=ALMGFQGVR-amide;
DE AltName: Full=AmTRP2;
DE AltName: Full=Tachykinin-related peptide 2;
DE Short=TRP2;
DE Contains:
DE RecName: Full=Brain peptide NSIINDVKNELFPEDIN;
DE Contains:
DE RecName: Full=APMGFQGMR-amide 1;
DE AltName: Full=AmTRP3;
DE AltName: Full=Tachykinin-related peptide 3-1;
DE Short=TRP3-1;
DE Contains:
DE RecName: Full=Brain peptide ASFDDEYY;
DE Contains:
DE RecName: Full=APMGFQGMR-amide 2;
DE AltName: Full=AmTRP3;
DE AltName: Full=Tachykinin-related peptide 3-2;
DE Short=TRP3-2;
DE Contains:
DE RecName: Full=Brain peptide SLEEILDEIK;
DE Contains:
DE RecName: Full=Brain peptide SLEEILDEI;
DE Contains:
DE RecName: Full=Brain peptide EILDEI;
DE Contains:
DE RecName: Full=TTRFQDSRSKDVYLIDYPEDY-amide;
DE AltName: Full=AmTAP1;
DE AltName: Full=Tachykinin-associated peptide 1;
DE Contains:
DE RecName: Full=Brain peptide VLSMDGYQNILDKKDELLGEWE;
DE Contains:
DE RecName: Full=Brain peptide APMGFYGT;
DE Contains:
DE RecName: Full=APMGFYGTR-amide;
DE AltName: Full=AmTRP4;
DE AltName: Full=Tachykinin-related peptide 4;
DE Short=TRP4;
DE Contains:
DE RecName: Full=Brain peptide IILDALEELD;
DE Contains:
DE RecName: Full=Brain peptide ILDALEELD;
DE Contains:
DE RecName: Full=Brain peptide GVMDFQIGLQ;
DE Contains:
DE RecName: Full=ARMGFHGMR-amide;
DE AltName: Full=AmTRP5;
DE AltName: Full=Tachykinin-related peptide 5;
DE Short=TRP5;
DE Contains:
DE RecName: Full=SPFRYLGAR-amide;
DE AltName: Full=AmTRP6;
DE AltName: Full=Tachykinin-related peptide 6;
DE Short=TRP6;
DE Contains:
DE RecName: Full=NPRWEFRGKFVGVR-amide;
DE AltName: Full=AmTRP7;
DE AltName: Full=Tachykinin-related peptide 7;
DE Short=TRP7;
DE Flags: Precursor;
GN Name=Atk {ECO:0000312|EMBL:BAC76402.1};
GN Synonyms=TK {ECO:0000303|PubMed:17068263};
OS Apis mellifera (Honeybee).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC Apis.
OX NCBI_TaxID=7460;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAC76402.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-38; 57-65; 88-96;
RP 110-118 AND 182-190, FUNCTION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC TISSUE=Brain {ECO:0000269|PubMed:12752663};
RX PubMed=12752663; DOI=10.1046/j.1365-2583.2003.00414.x;
RA Takeuchi H., Yasuda A., Yasuda-Kamatani Y., Kubo T., Nakajima T.;
RT "Identification of a tachykinin-related neuropeptide from the honeybee
RT brain using direct MALDI-TOF MS and its gene expression in worker, queen
RT and drone heads.";
RL Insect Mol. Biol. 12:291-298(2003).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 57-65; 69-85; 88-96; 100-107; 110-118; 122-131;
RP 134-154; 158-179; 182-190; 194-203; 206-215; 255-263; 304-312 AND 316-329,
RP AMIDATION AT GLN-38; ARG-65; ARG-96; ARG-118; TYR-154; ARG-190; ARG-263;
RP ARG-312 AND ARG-329, AND MASS SPECTROMETRY.
RC TISSUE=Brain {ECO:0000269|PubMed:17068263};
RX PubMed=17068263; DOI=10.1126/science.1124128;
RA Hummon A.B., Richmond T.A., Verleyen P., Baggerman G., Huybrechts J.,
RA Ewing M.A., Vierstraete E., Rodriguez-Zas S.L., Schoofs L., Robinson G.E.,
RA Sweedler J.V.;
RT "From the genome to the proteome: uncovering peptides in the Apis brain.";
RL Science 314:647-649(2006).
CC -!- FUNCTION: Tachykinins are active peptides which excite neurons, evoke
CC behavioral responses, are potent vasodilators and secretagogues, and
CC contract (directly or indirectly) many smooth muscles. Associated with
CC sex-specific or age/division of labor-selective behavior and/or
CC physiology of honeybees. {ECO:0000269|PubMed:12752663, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Strong expression was detected in the queen and
CC forager heads, while weak and almost no significant expression was
CC detected in the nurse and drone heads, respectively.
CC {ECO:0000269|PubMed:12752663}.
CC -!- MASS SPECTROMETRY: [ALMGFQGVR-amide]: Mass=976.53; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [ALMGFQGVR-amide]: Mass=976.53; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide NSIINDVKNELFPEDIN]: Mass=1972.97;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide NSIINDVKNELFPEDIN]: Mass=1972.97;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [APMGFQGMR-amide 1]: Mass=992.47;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:12752663,
CC ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [APMGFQGMR-amide 1]: Mass=992.47; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12752663, ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [APMGFQGMR-amide 1]: Mass=993.9; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12752663, ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide ASFDDEYY]: Mass=1008.38;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [APMGFQGMR-amide 2]: Mass=992.47; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12752663, ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [APMGFQGMR-amide 2]: Mass=992.47;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:12752663,
CC ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [APMGFQGMR-amide 2]: Mass=993.9; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12752663, ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide SLEEILDEI]: Mass=1059.54;
CC Method=Electrospray; Note=Incompletely processed peptide.;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide SLEEILDEIK]: Mass=1187.63;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide SLEEILDEIK]: Mass=1187.63;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide EILDEI]: Mass=730.38;
CC Method=Electrospray; Note=Partial peptide processing.;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [TTRFQDSRSKDVYLIDYPEDY-amide]: Mass=2609.24;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide VLSMDGYQNILDKKDELLGEWE]:
CC Mass=2594.26; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide APMGFYGT]: Mass=842.36;
CC Method=Electrospray; Note=Partial peptide processing.;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [APMGFYGTR-amide]: Mass=997.48; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [APMGFYGTR-amide]: Mass=997.48; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide IILDALEELD]: Mass=1142.61;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide ILDALEELD]: Mass=1029.50;
CC Method=Electrospray; Note=Partial peptide processing.;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [Brain peptide GVMDFQIGLQ]: Mass=1106.55;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [ARMGFHGMR-amide]: Mass=1060.52; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [SPFRYLGAR-amide]: Mass=1064.59;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [SPFRYLGAR-amide]: Mass=1064.59; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- MASS SPECTROMETRY: [NPRWEFRGKFVGVR-amide]: Mass=1745.96; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:17068263};
CC -!- SIMILARITY: Belongs to the tachykinin family. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC76399.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=BAC76400.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=BAC76401.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAC76401.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB073995; BAC76399.1; ALT_SEQ; mRNA.
DR EMBL; AB073996; BAC76400.1; ALT_SEQ; mRNA.
DR EMBL; AB073997; BAC76401.1; ALT_SEQ; mRNA.
DR EMBL; AB073998; BAC76402.1; -; mRNA.
DR RefSeq; NP_001011576.1; NM_001011576.1.
DR AlphaFoldDB; Q868G6; -.
DR SMR; Q868G6; -.
DR STRING; 7460.GB49248-PA; -.
DR PaxDb; Q868G6; -.
DR EnsemblMetazoa; NM_001011576; NP_001011576; GeneID_406083.
DR GeneID; 406083; -.
DR KEGG; ame:406083; -.
DR CTD; 21874; -.
DR eggNOG; ENOG502S9VB; Eukaryota.
DR InParanoid; Q868G6; -.
DR OrthoDB; 1528453at2759; -.
DR PhylomeDB; Q868G6; -.
DR Proteomes; UP000005203; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0007610; P:behavior; IEA:UniProtKB-KW.
DR GO; GO:0007268; P:chemical synaptic transmission; IEA:UniProtKB-KW.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Behavior; Cleavage on pair of basic residues;
KW Direct protein sequencing; Neuropeptide; Neurotransmitter;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT PROPEP 19..27
FT /evidence="ECO:0000255, ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343464"
FT PEPTIDE 30..38
FT /note="APTGHQEMQ-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343465"
FT PROPEP 41..54
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343466"
FT PEPTIDE 57..65
FT /note="ALMGFQGVR-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343467"
FT PEPTIDE 69..85
FT /note="Brain peptide NSIINDVKNELFPEDIN"
FT /id="PRO_0000343468"
FT PEPTIDE 88..96
FT /note="APMGFQGMR-amide 1"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343469"
FT PEPTIDE 100..107
FT /note="Brain peptide ASFDDEYY"
FT /id="PRO_0000343470"
FT PEPTIDE 110..118
FT /note="APMGFQGMR-amide 2"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343471"
FT PEPTIDE 122..131
FT /note="Brain peptide SLEEILDEIK"
FT /id="PRO_0000343472"
FT PEPTIDE 122..130
FT /note="Brain peptide SLEEILDEI"
FT /id="PRO_0000343473"
FT PEPTIDE 125..130
FT /note="Brain peptide EILDEI"
FT /id="PRO_0000343474"
FT PEPTIDE 134..154
FT /note="TTRFQDSRSKDVYLIDYPEDY-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343475"
FT PEPTIDE 158..179
FT /note="Brain peptide VLSMDGYQNILDKKDELLGEWE"
FT /id="PRO_0000343476"
FT PEPTIDE 182..190
FT /note="APMGFYGTR-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343477"
FT PEPTIDE 182..189
FT /note="Brain peptide APMGFYGT"
FT /id="PRO_0000343478"
FT PEPTIDE 194..203
FT /note="Brain peptide IILDALEELD"
FT /id="PRO_0000343479"
FT PEPTIDE 195..203
FT /note="Brain peptide ILDALEELD"
FT /id="PRO_0000343480"
FT PEPTIDE 206..215
FT /note="Brain peptide GVMDFQIGLQ"
FT /id="PRO_0000343481"
FT PROPEP 219..252
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343482"
FT PEPTIDE 255..263
FT /note="ARMGFHGMR-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343483"
FT PROPEP 267..301
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343484"
FT PEPTIDE 304..312
FT /note="SPFRYLGAR-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343485"
FT PEPTIDE 316..329
FT /note="NPRWEFRGKFVGVR-amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343486"
FT PROPEP 333..339
FT /evidence="ECO:0000269|PubMed:17068263"
FT /id="PRO_0000343487"
FT MOD_RES 38
FT /note="Glutamine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 65
FT /note="Arginine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 96
FT /note="Arginine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 118
FT /note="Arginine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 154
FT /note="Tyrosine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 190
FT /note="Arginine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 263
FT /note="Arginine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 312
FT /note="Arginine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT MOD_RES 329
FT /note="Arginine amide"
FT /evidence="ECO:0000269|PubMed:17068263"
FT CONFLICT 6..7
FT /note="IF -> TL (in Ref. 1; BAC76401)"
FT /evidence="ECO:0000305"
FT CONFLICT 41
FT /note="Q -> EK (in Ref. 1; BAC76401)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 339 AA; 39039 MW; E6C10381B76C77EE CRC64;
MIIHSIFLLM VSITLVIAEE SDNVLFDKRA PTGHQEMQGK QNSASLNSEN FGIFKRALMG
FQGVRGKKNS IINDVKNELF PEDINKRAPM GFQGMRGKKA SFDDEYYKRA PMGFQGMRGK
KSLEEILDEI KKKTTRFQDS RSKDVYLIDY PEDYGKRVLS MDGYQNILDK KDELLGEWEK
RAPMGFYGTR GKKIILDALE ELDKRGVMDF QIGLQRKKDT TFDDYLDYAI NPFDYEKRST
DFQDVESGSE SFKRARMGFH GMRGKRDAAG IYGSNSSTVG TIFGYQDMRN RGNNFPVYQV
EKRSPFRYLG ARGKKNPRWE FRGKFVGVRG KKSSLQTVF
