BPHY_PSEAE
ID BPHY_PSEAE Reviewed; 728 AA.
AC Q9HWR3;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Bacteriophytochrome;
DE EC=2.7.13.3;
DE AltName: Full=Phytochrome-like protein;
GN Name=bphP; OrderedLocusNames=PA4117;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Photoreceptor which exists in two forms that are reversibly
CC interconvertible by light: the R form that absorbs maximally in the red
CC region of the spectrum and the FR form that absorbs maximally in the
CC far-red region. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- INTERACTION:
CC Q9HWR3; Q9HWR3: bphP; NbExp=4; IntAct=EBI-15729881, EBI-15729881;
CC -!- PTM: Contains one covalently linked tetrapyrrole chromophore.
CC {ECO:0000250}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the phytochrome
CC family. {ECO:0000305}.
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DR EMBL; AE004091; AAG07504.1; -; Genomic_DNA.
DR PIR; B83131; B83131.
DR RefSeq; NP_252806.1; NC_002516.2.
DR RefSeq; WP_003101278.1; NZ_QZGE01000013.1.
DR PDB; 3C2W; X-ray; 2.90 A; A/B/C/D/E/F/G/H=1-497.
DR PDB; 3G6O; X-ray; 2.85 A; A/B=1-497.
DR PDB; 3IBR; X-ray; 2.97 A; A/B=1-497.
DR PDB; 3NHQ; X-ray; 2.55 A; A/B/C/D/E/F/G/H=1-497.
DR PDB; 3NOP; X-ray; 2.80 A; C=1-497.
DR PDB; 3NOT; X-ray; 2.70 A; C=1-497.
DR PDB; 3NOU; X-ray; 3.00 A; C=1-497.
DR PDBsum; 3C2W; -.
DR PDBsum; 3G6O; -.
DR PDBsum; 3IBR; -.
DR PDBsum; 3NHQ; -.
DR PDBsum; 3NOP; -.
DR PDBsum; 3NOT; -.
DR PDBsum; 3NOU; -.
DR AlphaFoldDB; Q9HWR3; -.
DR SMR; Q9HWR3; -.
DR DIP; DIP-46175N; -.
DR STRING; 287.DR97_3754; -.
DR PaxDb; Q9HWR3; -.
DR PRIDE; Q9HWR3; -.
DR EnsemblBacteria; AAG07504; AAG07504; PA4117.
DR GeneID; 880365; -.
DR KEGG; pae:PA4117; -.
DR PATRIC; fig|208964.12.peg.4314; -.
DR PseudoCAP; PA4117; -.
DR HOGENOM; CLU_000445_50_1_6; -.
DR InParanoid; Q9HWR3; -.
DR OMA; YMRNIGM; -.
DR PhylomeDB; Q9HWR3; -.
DR BioCyc; PAER208964:G1FZ6-4190-MON; -.
DR BRENDA; 2.7.13.3; 5087.
DR EvolutionaryTrace; Q9HWR3; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0009881; F:photoreceptor activity; IEA:UniProtKB-KW.
DR GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central.
DR GO; GO:0009584; P:detection of visible light; IEA:InterPro.
DR GO; GO:0007234; P:osmosensory signaling via phosphorelay pathway; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.450.270; -; 1.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013654; PAS_2.
DR InterPro; IPR016132; Phyto_chromo_attachment.
DR InterPro; IPR001294; Phytochrome.
DR InterPro; IPR013515; Phytochrome_cen-reg.
DR InterPro; IPR043150; Phytochrome_PHY.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF08446; PAS_2; 1.
DR Pfam; PF00360; PHY; 1.
DR PRINTS; PR01033; PHYTOCHROME.
DR SMART; SM00065; GAF; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
DR PROSITE; PS50046; PHYTOCHROME_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Chromophore; Kinase; Nucleotide-binding;
KW Phosphoprotein; Photoreceptor protein; Receptor; Reference proteome;
KW Sensory transduction; Transferase.
FT CHAIN 1..728
FT /note="Bacteriophytochrome"
FT /id="PRO_0000172000"
FT DOMAIN 139..303
FT /note="GAF"
FT DOMAIN 510..721
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT REGION 17..495
FT /note="Chromophore binding domain"
FT BINDING 12
FT /ligand="a tetrapyrrole"
FT /ligand_id="ChEBI:CHEBI:26932"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000250"
FT MOD_RES 513
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT TURN 9..11
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 12..14
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 21..23
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 27..32
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 36..42
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 45..49
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 61..76
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 85..88
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 90..93
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 95..102
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 105..112
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 121..136
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 140..155
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 158..165
FT /evidence="ECO:0007829|PDB:3NHQ"
FT TURN 167..169
FT /evidence="ECO:0007829|PDB:3G6O"
FT STRAND 171..178
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 192..194
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 197..205
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 207..212
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 219..225
FT /evidence="ECO:0007829|PDB:3NHQ"
FT TURN 227..229
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 230..232
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 235..237
FT /evidence="ECO:0007829|PDB:3G6O"
FT STRAND 239..241
FT /evidence="ECO:0007829|PDB:3G6O"
FT HELIX 245..254
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 258..265
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 267..280
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 286..332
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 335..340
FT /evidence="ECO:0007829|PDB:3NHQ"
FT TURN 342..344
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 346..349
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 353..359
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 362..368
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 371..382
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 388..393
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 410..417
FT /evidence="ECO:0007829|PDB:3NHQ"
FT TURN 418..421
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 422..428
FT /evidence="ECO:0007829|PDB:3NHQ"
FT STRAND 434..440
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 452..454
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 456..467
FT /evidence="ECO:0007829|PDB:3NHQ"
FT HELIX 476..493
FT /evidence="ECO:0007829|PDB:3NHQ"
SQ SEQUENCE 728 AA; 80931 MW; EEE9259392E5C4B0 CRC64;
MTSITPVTLA NCEDEPIHVP GAIQPHGALV TLRADGMVLA ASENIQALLG FVASPGSYLT
QEQVGPEVLR MLEEGLTGNG PWSNSVETRI GEHLFDVIGH SYKEVFYLEF EIRTADTLSI
TSFTLNAQRI IAQVQLHNDT ASLLSNVTDE LRRMTGYDRV MAYRFRHDDS GEVVAESRRE
DLESYLGQRY PASDIPAQAR RLYIQNPIRL IADVAYTPMR VFPALNPETN ESFDLSYSVL
RSVSPIHCEY LTNMGVRASM SISIVVGGKL WGLFSCHHMS PKLIPYPVRM SFQIFSQVCS
AIVERLEQGR IAELLRVSTE RRLALARRAR DADDLFGALA HPDDGIAALI PCDGALVMLG
GRTLSIRGDF ERQAGNVLQR LQRDPERDIY HTDNWPQPSE DSPDGGDCCG VLAIRFHRQE
SGWIFWFRHE EVHRIRWGGK PEKLLTIGPS GPRLTPRGSF EAWEEVVRGH STPWSETDLA
IAEKLRLDLM ELCLNHAAEV DRMRQRLIAV LGHDLRNPLQ SISMAAALLS SSDTRTTELR
QHISASSSRM ERLVSQILDM SRLQSGIGLT VNPVDTDVSQ LVRQIVCETD VAYPGLVIEI
AIDPQVRAVV DPDRYAQVAA NLLSNARHHG LPGRPVLVTL TRQGDEVCLS VLNETSGLSE
AQLANLFEPF KRESADNQRN RNGLGIGLYI SQAIAQAHQG RIDVDCRDDV ITFCLRLPVR
QAETGSSS
