TAD3_YEAST
ID TAD3_YEAST Reviewed; 322 AA.
AC Q9URQ3; D6VYW0; O13552;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=tRNA-specific adenosine deaminase subunit TAD3;
DE AltName: Full=tRNA-specific adenosine-34 deaminase subunit TAD3;
GN Name=TAD3; OrderedLocusNames=YLR316C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=BMA41;
RX PubMed=10550050; DOI=10.1126/science.286.5442.1146;
RA Gerber A.P., Keller W.;
RT "An adenosine deaminase that generates inosine at the wobble position of
RT transfer RNAs.";
RL Science 286:1146-1149(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Deaminates adenosine-34 to inosine in many tRNAs.
CC -!- SUBUNIT: Heterodimer with TAD2.
CC -!- INTERACTION:
CC Q9URQ3; P47058: TAD2; NbExp=2; IntAct=EBI-2094330, EBI-18939;
CC -!- MISCELLANEOUS: Present with 892 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase
CC family. ADAT3 subfamily. {ECO:0000305}.
CC -!- CAUTION: In contrast to other cytidine and deoxycytidylate deaminase,
CC lacks to conserved Glu active site in position 218 which is replaced by
CC a Val residue, suggesting that it acts as a regulatory subunit.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB64529.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ242668; CAB60630.1; -; Genomic_DNA.
DR EMBL; U20618; AAB64529.1; ALT_INIT; Genomic_DNA.
DR EMBL; BK006945; DAA09626.1; -; Genomic_DNA.
DR RefSeq; NP_013420.2; NM_001182205.1.
DR PDB; 7BV5; X-ray; 2.80 A; C/D=1-322.
DR PDBsum; 7BV5; -.
DR AlphaFoldDB; Q9URQ3; -.
DR SMR; Q9URQ3; -.
DR BioGRID; 31581; 573.
DR ComplexPortal; CPX-1742; tRNA-specific adenosine-34 deaminase complex.
DR DIP; DIP-5917N; -.
DR IntAct; Q9URQ3; 1.
DR STRING; 4932.YLR316C; -.
DR MaxQB; Q9URQ3; -.
DR PaxDb; Q9URQ3; -.
DR PRIDE; Q9URQ3; -.
DR EnsemblFungi; YLR316C_mRNA; YLR316C; YLR316C.
DR GeneID; 851027; -.
DR KEGG; sce:YLR316C; -.
DR SGD; S000004308; TAD3.
DR VEuPathDB; FungiDB:YLR316C; -.
DR eggNOG; KOG2771; Eukaryota.
DR GeneTree; ENSGT00390000010706; -.
DR HOGENOM; CLU_013817_2_0_1; -.
DR InParanoid; Q9URQ3; -.
DR OMA; GDGYCMH; -.
DR BioCyc; YEAST:YLR316C-MON; -.
DR BRENDA; 3.5.4.33; 984.
DR PRO; PR:Q9URQ3; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q9URQ3; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0052718; C:tRNA-specific adenosine-34 deaminase complex; IPI:ComplexPortal.
DR GO; GO:0008251; F:tRNA-specific adenosine deaminase activity; IDA:SGD.
DR GO; GO:0006400; P:tRNA modification; IDA:SGD.
DR GO; GO:0002100; P:tRNA wobble adenosine to inosine editing; IDA:ComplexPortal.
DR InterPro; IPR002125; CMP_dCMP_dom.
DR InterPro; IPR016193; Cytidine_deaminase-like.
DR Pfam; PF00383; dCMP_cyt_deam_1; 1.
DR SUPFAM; SSF53927; SSF53927; 1.
DR PROSITE; PS51747; CYT_DCMP_DEAMINASES_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; tRNA processing.
FT CHAIN 1..322
FT /note="tRNA-specific adenosine deaminase subunit TAD3"
FT /id="PRO_0000171741"
FT DOMAIN 162..283
FT /note="CMP/dCMP-type deaminase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01083"
FT TURN 13..16
FT /evidence="ECO:0007829|PDB:7BV5"
FT TURN 19..21
FT /evidence="ECO:0007829|PDB:7BV5"
FT STRAND 22..24
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 29..32
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 46..63
FT /evidence="ECO:0007829|PDB:7BV5"
FT STRAND 78..83
FT /evidence="ECO:0007829|PDB:7BV5"
FT TURN 90..92
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 96..101
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 102..104
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 129..138
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 148..155
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 160..179
FT /evidence="ECO:0007829|PDB:7BV5"
FT STRAND 187..191
FT /evidence="ECO:0007829|PDB:7BV5"
FT STRAND 200..203
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 205..207
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 217..231
FT /evidence="ECO:0007829|PDB:7BV5"
FT TURN 241..244
FT /evidence="ECO:0007829|PDB:7BV5"
FT STRAND 246..251
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 255..263
FT /evidence="ECO:0007829|PDB:7BV5"
FT STRAND 267..273
FT /evidence="ECO:0007829|PDB:7BV5"
FT TURN 276..278
FT /evidence="ECO:0007829|PDB:7BV5"
FT STRAND 280..282
FT /evidence="ECO:0007829|PDB:7BV5"
FT HELIX 291..293
FT /evidence="ECO:0007829|PDB:7BV5"
FT STRAND 302..306
FT /evidence="ECO:0007829|PDB:7BV5"
FT STRAND 308..311
FT /evidence="ECO:0007829|PDB:7BV5"
SQ SEQUENCE 322 AA; 37107 MW; 08D0878F10B36403 CRC64;
MVKKVNNPLK IDYQNGIIEN RLLQIRNFKD VNTPKLINVW SIRIDPRDSK KVIELIRNDF
QKNDPVSLRH LKRIRKDIET STLEVVLCSK EYICDEGEIN NKLKSIWVGT KKYELSDDIE
VPEFAPSTKE LNNAWSVKYW PLIWNGNPND QILNDYKIDM QEVRNELSRA STLSVKMATA
GKQFPMVSVF VDPSRKKDKV VAEDGRNCEN SLPIDHSVMV GIRAVGERLR EGVDEDANSY
LCLDYDVYLT HEPCSMCSMA LIHSRVRRVV FLTEMQRTGS LKLTSGDGYC MNDNKQLNST
YEAFQWIGEE YPVGQVDRDV CC
