BPIA1_RAT
ID BPIA1_RAT Reviewed; 270 AA.
AC Q8K4I4;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=BPI fold-containing family A member 1;
DE AltName: Full=Palate lung and nasal epithelium clone protein;
DE Flags: Precursor;
GN Name=Bpifa1; Synonyms=Plunc;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=Sprague-Dawley; TISSUE=Nasal epithelium;
RX PubMed=11821380; DOI=10.1074/jbc.m106208200;
RA Sung Y.K., Moon C., Yoo J.-Y., Moon C., Pearse D., Pevsner J.,
RA Ronnett G.V.;
RT "Plunc, a member of the secretory gland protein family, is up-regulated in
RT nasal respiratory epithelium after olfactory bulbectomy.";
RL J. Biol. Chem. 277:12762-12769(2002).
CC -!- FUNCTION: Lipid-binding protein which shows high specificity for the
CC surfactant phospholipid dipalmitoylphosphatidylcholine (DPPC). Plays a
CC role in the innate immune responses of the upper airways. Reduces the
CC surface tension in secretions from airway epithelia and inhibits the
CC formation of biofilm by pathogenic Gram-negative bacteria, such as
CC P.aeruginosa and K.pneumoniae. Negatively regulates proteolytic
CC cleavage of SCNN1G, an event that is required for activation of the
CC epithelial sodium channel (ENaC), and thereby contributes to airway
CC surface liquid homeostasis and proper clearance of mucus. Plays a role
CC in the airway inflammatory response after exposure to irritants. May
CC attract macrophages and neutrophils. {ECO:0000250|UniProtKB:Q9NP55}.
CC -!- SUBUNIT: Monomer. Interacts (via N-terminus) with SCNN1B, a subunit of
CC the heterotrimeric epithelial sodium channel (ENaC); this inhibits
CC proteolytic activation of ENaC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11821380}.
CC Note=Apical side of airway epithelial cells. Detected in airway surface
CC liquid, nasal mucus and sputum.
CC -!- TISSUE SPECIFICITY: Detected in adult nasal epithelium, heart, lung,
CC spleen, testis and salivary gland, and in embryonic nasal epithelium,
CC lung, salivary gland and thymus. {ECO:0000269|PubMed:11821380}.
CC -!- INDUCTION: After bulbectomy or lesion of the olfactory bulb.
CC {ECO:0000269|PubMed:11821380}.
CC -!- SIMILARITY: Belongs to the BPI/LBP/Plunc superfamily. Plunc family.
CC {ECO:0000305}.
CC -!- CAUTION: Reported to bind to bacterial lipopolysaccharide (LPS) in
CC vitro. However, the in vivo significance of this is uncertain since
CC other studies indicate little or no specificity for LPS.
CC {ECO:0000250|UniProtKB:Q9NP55}.
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DR EMBL; AF393750; AAM73687.1; -; mRNA.
DR RefSeq; NP_742028.1; NM_172031.1.
DR AlphaFoldDB; Q8K4I4; -.
DR SMR; Q8K4I4; -.
DR STRING; 10116.ENSRNOP00000018581; -.
DR GlyGen; Q8K4I4; 1 site.
DR PaxDb; Q8K4I4; -.
DR Ensembl; ENSRNOT00000018581; ENSRNOP00000018581; ENSRNOG00000013859.
DR GeneID; 246238; -.
DR KEGG; rno:246238; -.
DR UCSC; RGD:619818; rat.
DR CTD; 51297; -.
DR RGD; 619818; Bpifa1.
DR eggNOG; ENOG502SR58; Eukaryota.
DR GeneTree; ENSGT01020000230460; -.
DR HOGENOM; CLU_095915_0_0_1; -.
DR InParanoid; Q8K4I4; -.
DR OMA; ANMLIHG; -.
DR OrthoDB; 1275829at2759; -.
DR PhylomeDB; Q8K4I4; -.
DR TreeFam; TF337052; -.
DR Reactome; R-RNO-6803157; Antimicrobial peptides.
DR PRO; PR:Q8K4I4; -.
DR Proteomes; UP000002494; Chromosome 3.
DR Bgee; ENSRNOG00000013859; Expressed in lung and 4 other tissues.
DR Genevisible; Q8K4I4; RN.
DR GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0005902; C:microvillus; IDA:RGD.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0019731; P:antibacterial humoral response; ISS:UniProtKB.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; ISO:RGD.
DR GO; GO:0051607; P:defense response to virus; ISO:RGD.
DR GO; GO:0002395; P:immune response in nasopharyngeal-associated lymphoid tissue; ISO:RGD.
DR GO; GO:0045087; P:innate immune response; ISS:UniProtKB.
DR GO; GO:0050891; P:multicellular organismal water homeostasis; ISS:UniProtKB.
DR GO; GO:1900229; P:negative regulation of single-species biofilm formation in or on host organism; ISS:UniProtKB.
DR GO; GO:0050828; P:regulation of liquid surface tension; ISS:UniProtKB.
DR GO; GO:1902305; P:regulation of sodium ion transmembrane transport; ISS:UniProtKB.
DR InterPro; IPR017943; Bactericidal_perm-incr_a/b_dom.
DR InterPro; IPR034307; BPIFA1.
DR InterPro; IPR017942; Lipid-bd_serum_glycop_N.
DR PANTHER; PTHR47015:SF1; PTHR47015:SF1; 1.
DR Pfam; PF01273; LBP_BPI_CETP; 1.
DR SUPFAM; SSF55394; SSF55394; 1.
PE 2: Evidence at transcript level;
KW Antibiotic; Antimicrobial; Disulfide bond; Glycoprotein; Immunity;
KW Innate immunity; Lipid-binding; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..270
FT /note="BPI fold-containing family A member 1"
FT /id="PRO_0000017178"
FT REGION 104..109
FT /note="Important for surfactant activity and antibacterial
FT properties"
FT /evidence="ECO:0000250|UniProtKB:Q9NP55"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 196..238
FT /evidence="ECO:0000250|UniProtKB:Q9NP55"
SQ SEQUENCE 270 AA; 27719 MW; 17B5EA3D4C845524 CRC64;
MFLVGSLVVL CGLLAQSTAQ LAGLPLPLGQ GLPLPLGQGL PLPLGQGLPL AVSPALPSNP
TDLLAGNFAN ALSGGLLSGG LLGILENIPL LDVIKSGGGS SNGLVGGLLG KLTSSVPLLN
NILDIKITDP RLLELGLVQS PDGHRLYATI PLSLKLQVNM PVVGSFLQLA VKLNITAEIV
AMKDNQGRIH LVLGDCTHSP GSLQITLLNG VTPVQSSLDS LTGILTKVLP ELIQGKVCPL
INGILSGLDV TLVHNIAELL IHGIQFVIKV
