BPIA2_MOUSE
ID BPIA2_MOUSE Reviewed; 235 AA.
AC P07743; A2AIF8; Q9D734;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=BPI fold-containing family A member 2;
DE AltName: Full=Parotid secretory protein;
DE Short=PSP;
DE Flags: Precursor;
GN Name=Bpifa2; Synonyms=Psp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Parotid gland;
RX PubMed=2582349; DOI=10.1093/nar/13.1.1;
RA Madsen H.O., Hjorth J.P.;
RT "Molecular cloning of mouse PSP mRNA.";
RL Nucleic Acids Res. 13:1-13(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-87.
RC STRAIN=C3H/HeJ; TISSUE=Spleen;
RX PubMed=2428613; DOI=10.1002/j.1460-2075.1986.tb04441.x;
RA Poulsen K., Jakobsen B.K., Mikkelsen B.M., Harmark K., Nielsen J.T.,
RA Hjorth J.P.;
RT "Coordination of murine parotid secretory protein and salivary amylase
RT expression.";
RL EMBO J. 5:1891-1896(1986).
RN [6]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=9461541; DOI=10.1042/bj3300437;
RA Mirels L., Miranda A.J., Ball W.D.;
RT "Characterization of the rat salivary-gland B1-immunoreactive proteins.";
RL Biochem. J. 330:437-444(1998).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Has strong antibacterial activity against P. aeruginosa.
CC {ECO:0000250|UniProtKB:Q96DR5}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9461541}.
CC -!- TISSUE SPECIFICITY: Predominates in the parotid glands, present in
CC smaller amounts (1/10) in the submaxillary glands and in the sublingual
CC glands, and at lower amount in the pancreas but undetectable in the
CC liver. Found also in lacrimal gland. {ECO:0000269|PubMed:9461541}.
CC -!- DEVELOPMENTAL STAGE: Expressed at low levels at day 3 after birth but
CC increased gradually with age from this time.
CC -!- SIMILARITY: Belongs to the BPI/LBP/Plunc superfamily. Plunc family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X01697; CAA25846.1; -; mRNA.
DR EMBL; M26807; AAA40009.1; -; Genomic_DNA.
DR EMBL; M26806; AAA40009.1; JOINED; Genomic_DNA.
DR EMBL; AK009032; BAB26037.1; -; mRNA.
DR EMBL; AK009654; BAB26418.1; -; mRNA.
DR EMBL; AK010181; BAB26753.1; -; mRNA.
DR EMBL; AL732466; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC010288; AAH10288.1; -; mRNA.
DR EMBL; BC053336; AAH53336.1; -; mRNA.
DR CCDS; CCDS16923.1; -.
DR PIR; A23031; SQMS.
DR RefSeq; NP_032979.1; NM_008953.2.
DR AlphaFoldDB; P07743; -.
DR SMR; P07743; -.
DR STRING; 10090.ENSMUSP00000046080; -.
DR CPTAC; non-CPTAC-3553; -.
DR MaxQB; P07743; -.
DR PaxDb; P07743; -.
DR PeptideAtlas; P07743; -.
DR PRIDE; P07743; -.
DR ProteomicsDB; 281702; -.
DR Antibodypedia; 25522; 197 antibodies from 24 providers.
DR Ensembl; ENSMUST00000048103; ENSMUSP00000046080; ENSMUSG00000042459.
DR GeneID; 19194; -.
DR KEGG; mmu:19194; -.
DR UCSC; uc008nis.1; mouse.
DR CTD; 140683; -.
DR MGI; MGI:97787; Bpifa2.
DR VEuPathDB; HostDB:ENSMUSG00000042459; -.
DR eggNOG; ENOG502TE6F; Eukaryota.
DR GeneTree; ENSGT01020000230460; -.
DR InParanoid; P07743; -.
DR OMA; TVIVGKC; -.
DR OrthoDB; 1389938at2759; -.
DR PhylomeDB; P07743; -.
DR TreeFam; TF337052; -.
DR Reactome; R-MMU-6803157; Antimicrobial peptides.
DR BioGRID-ORCS; 19194; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Bpifa2; mouse.
DR PRO; PR:P07743; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; P07743; protein.
DR Bgee; ENSMUSG00000042459; Expressed in parotid gland and 36 other tissues.
DR ExpressionAtlas; P07743; baseline and differential.
DR Genevisible; P07743; MM.
DR GO; GO:0005576; C:extracellular region; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0030141; C:secretory granule; ISO:MGI.
DR GO; GO:0001530; F:lipopolysaccharide binding; ISO:MGI.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR InterPro; IPR017943; Bactericidal_perm-incr_a/b_dom.
DR InterPro; IPR017942; Lipid-bd_serum_glycop_N.
DR Pfam; PF01273; LBP_BPI_CETP; 1.
DR SUPFAM; SSF55394; SSF55394; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Disulfide bond; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..235
FT /note="BPI fold-containing family A member 2"
FT /id="PRO_0000017185"
FT DISULFID 161..204
FT /evidence="ECO:0000250"
FT CONFLICT 4
FT /note="L -> P (in Ref. 2; BAB26418)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 235 AA; 24753 MW; 23311BAE1E6E2EF3 CRC64;
MFQLGSLVVL CGLLIGNSES LLGELGSAVN NLKILNPPSE AVPQNLNLDV ELLQQATSWP
LAKNSILETL NTADLGNLKS FTSLNGLLLK INNLKVLDFQ AKLSSNGNGI DLTVPLAGEA
SLVLPFIGKT VDISVSLDLI NSLSIKTNAQ TGLPEVTIGK CSSNTDKISI SLLGRRLPII
NSILDGVSTL LTSTLSTVLQ NFLCPLLQYV LSTLNPSVLQ GLLSNLLAGQ VQLAL
