TAF14_ARATH
ID TAF14_ARATH Reviewed; 268 AA.
AC F4IPK2; Q9SL46;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Transcription initiation factor TFIID subunit 14 {ECO:0000303|PubMed:15527982};
DE AltName: Full=Protein AF-9 homolog b {ECO:0000303|PubMed:23017898};
DE AltName: Full=TBP-associated factor 14 {ECO:0000303|PubMed:15527982};
DE Short=AtTAF14 {ECO:0000303|PubMed:15527982};
GN Name=TAF14 {ECO:0000303|PubMed:15527982};
GN Synonyms=YAF9B {ECO:0000303|PubMed:23017898};
GN OrderedLocusNames=At2g18000 {ECO:0000312|Araport:AT2G18000};
GN ORFNames=T27K22.13 {ECO:0000312|EMBL:AAD20126.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, NOMENCLATURE, AND TISSUE
RP SPECIFICITY.
RX PubMed=15527982; DOI=10.1016/j.gene.2004.08.023;
RA Lago C., Clerici E., Mizzi L., Colombo L., Kater M.M.;
RT "TBP-associated factors in Arabidopsis.";
RL Gene 342:231-241(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND INTERACTION WITH TAF4B; TAF5;
RP TAF12; TAF8; TAF12; TAF12B; TAF13 AND TAF15B.
RC STRAIN=cv. Columbia;
RX PubMed=17340043; DOI=10.1007/s11103-007-9135-1;
RA Lawit S.J., O'Grady K., Gurley W.B., Czarnecka-Verner E.;
RT "Yeast two-hybrid map of Arabidopsis TFIID.";
RL Plant Mol. Biol. 64:73-87(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH THE FRI-C COMPLEX AND
RP SWC6, AND DISRUPTION PHENOTYPE.
RX PubMed=21282526; DOI=10.1105/tpc.110.075911;
RA Choi K., Kim J., Hwang H.J., Kim S., Park C., Kim S.Y., Lee I.;
RT "The FRIGIDA complex activates transcription of FLC, a strong flowering
RT repressor in Arabidopsis, by recruiting chromatin modification factors.";
RL Plant Cell 23:289-303(2011).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23017898; DOI=10.1016/j.plantsci.2012.07.010;
RA Zacharaki V., Benhamed M., Poulios S., Latrasse D., Papoutsoglou P.,
RA Delarue M., Vlachonasios K.E.;
RT "The Arabidopsis ortholog of the YEATS domain containing protein YAF9a
RT regulates flowering by controlling H4 acetylation levels at the FLC
RT locus.";
RL Plant Sci. 196:44-52(2012).
RN [7]
RP FUNCTION, IDENTIFICATION IN THE NUA4/SWR1 COMPLEX, INTERACTION WITH EAF1A
RP AND EAF1B, AND DISRUPTION PHENOTYPE.
RX DOI=10.1186/s12870-015-0461-1;
RA Bieluszewski T., Galganski L., Sura W., Bieluszewska A., Abram M.,
RA Ludwikow A., Ziolkowski P., Sadowski J.;
RT "AtEAF1 is a potential platform protein for Arabidopsis NuA4
RT acetyltransferase complex.";
RL BMC Plant Biol. 15:0-0(2015).
CC -!- FUNCTION: Negative regulator of flowering controlling the H4K5
CC acetylation levels in the FLC and FT chromatin. Positively regulates
CC FLC expression. Component of the transcription factor IID (TFIID)
CC complex that is essential for mediating regulation of RNA polymerase
CC transcription. Component of the SWR1 complex which mediates the ATP-
CC dependent exchange of histone H2A for the H2A variant HZT1 leading to
CC transcriptional regulation of selected genes by chromatin remodeling.
CC Component of a NuA4 histone acetyltransferase complex which is involved
CC in transcriptional activation of selected genes principally by
CC acetylation of nucleosomal histones H4 and H2A.
CC {ECO:0000269|PubMed:23017898, ECO:0000269|Ref.7}.
CC -!- SUBUNIT: Component of the TFIID complex. TFIID is composed of TATA
CC binding protein (TBP) and a number of TBP-associated factors (TAFs)
CC whose MWs range from 14-217 kDa. Component of the SWR1 chromatin-
CC remodeling complex. Interacts with TAF4B, TAF5, TAF8, TAF12, TAF12B,
CC TAF13 and TAF15B. Interacts with the transcription activator complex
CC FRI-C. Interacts with SWC6, FRI and (via C-terminus) with FLX, SUF4,
CC FRIL1 and FES1 (PubMed:17340043, PubMed:21282526). Interacts with EAF1A
CC and EAF1B (via HSA domain) (Ref.7). {ECO:0000269|PubMed:17340043,
CC ECO:0000269|PubMed:21282526, ECO:0000269|Ref.7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4IPK2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4IPK2-2; Sequence=VSP_053265, VSP_053266;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, inflorescence and
CC flowering tissues. {ECO:0000269|PubMed:15527982,
CC ECO:0000269|PubMed:23017898}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, due to the redundancy with
CC TAF14B (PubMed:21282526). Decreased FLC expression leading to earlier
CC transition from vegetative to reproductive phase (Ref.7). Taf14 and
CC taf14b double mutants show a pleiotropic phenotype that includes small
CC size and abnormal leaf morphology (PubMed:21282526). Taf14 and taf14b
CC double mutants flower significantly earlier than single mutants under
CC both long days and short days conditions (Ref.7).
CC {ECO:0000269|PubMed:21282526, ECO:0000269|Ref.7}.
CC -!- SIMILARITY: Belongs to the YAF9 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY463616; AAR28018.1; -; mRNA.
DR EMBL; AC006201; AAD20126.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06713.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06714.1; -; Genomic_DNA.
DR PIR; A84559; A84559.
DR RefSeq; NP_001189547.1; NM_001202618.2. [F4IPK2-1]
DR RefSeq; NP_179391.1; NM_127356.1. [F4IPK2-2]
DR AlphaFoldDB; F4IPK2; -.
DR SMR; F4IPK2; -.
DR BioGRID; 1669; 16.
DR IntAct; F4IPK2; 9.
DR STRING; 3702.AT2G18000.2; -.
DR PaxDb; F4IPK2; -.
DR PRIDE; F4IPK2; -.
DR ProteomicsDB; 245285; -. [F4IPK2-1]
DR EnsemblPlants; AT2G18000.1; AT2G18000.1; AT2G18000. [F4IPK2-2]
DR EnsemblPlants; AT2G18000.2; AT2G18000.2; AT2G18000. [F4IPK2-1]
DR GeneID; 816312; -.
DR Gramene; AT2G18000.1; AT2G18000.1; AT2G18000. [F4IPK2-2]
DR Gramene; AT2G18000.2; AT2G18000.2; AT2G18000. [F4IPK2-1]
DR KEGG; ath:AT2G18000; -.
DR Araport; AT2G18000; -.
DR TAIR; locus:2060938; AT2G18000.
DR eggNOG; KOG3149; Eukaryota.
DR HOGENOM; CLU_051385_1_1_1; -.
DR InParanoid; F4IPK2; -.
DR OMA; NPENEYG; -.
DR OrthoDB; 1482359at2759; -.
DR PRO; PR:F4IPK2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IPK2; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0035267; C:NuA4 histone acetyltransferase complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006338; P:chromatin remodeling; IBA:GO_Central.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0016573; P:histone acetylation; IBA:GO_Central.
DR GO; GO:0043981; P:histone H4-K5 acetylation; IMP:UniProtKB.
DR GO; GO:0009909; P:regulation of flower development; IMP:UniProtKB.
DR GO; GO:2000028; P:regulation of photoperiodism, flowering; IGI:TAIR.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 2.60.40.1970; -; 1.
DR InterPro; IPR038704; YEAST_sf.
DR InterPro; IPR005033; YEATS.
DR PANTHER; PTHR23195; PTHR23195; 1.
DR Pfam; PF03366; YEATS; 1.
DR PROSITE; PS51037; YEATS; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Coiled coil; Cytoplasm;
KW Developmental protein; Differentiation; Flowering; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..268
FT /note="Transcription initiation factor TFIID subunit 14"
FT /id="PRO_0000423727"
FT DOMAIN 34..180
FT /note="YEATS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00376"
FT COILED 227..252
FT /evidence="ECO:0000255"
FT VAR_SEQ 199..202
FT /note="VADT -> AFLF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17340043"
FT /id="VSP_053265"
FT VAR_SEQ 203..268
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17340043"
FT /id="VSP_053266"
SQ SEQUENCE 268 AA; 30363 MW; DA8468F305F48DE9 CRC64;
MESDIEILSE ADASMRKLRI FGIDDREDEN GRRRIKDVEV YVPIVCGSIA FYLGKKATEY
RTHKWTVYVR GATNEDLGVV IKRVIFHLHP SFNNPTRVVD APPFALSECG WGEFKIDITV
FFHTDVCEKK LELSHVLKLN PENAYGPIPK SIKIPVVAES YNEVVFPDPF ESFVARVHNH
PAIQISNIPD GLNLPPPGVA DTYYLMEKGD TKEHPLSPWF LKFSEVEELF KLTAARQKVQ
ADIAKLKRQL IMVDGQPEGL ESSSGYEC
