TAF1B_CAEBR
ID TAF1B_CAEBR Reviewed; 889 AA.
AC A8XLS0;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=TATA box-binding protein-associated factor RNA polymerase I subunit B;
DE AltName: Full=TATA box-binding protein-associated factor 1B;
DE Short=TBP-associated factor 1B;
GN ORFNames=CBG15174;
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Component of RNA polymerase I core factor complex that acts
CC as a GTF2B/TFIIB-like factor and plays a key role in multiple steps
CC during transcription initiation such as pre-initiation complex (PIC)
CC assembly and postpolymerase recruitment events in polymerase I (Pol I)
CC transcription. Binds rDNA promoters and plays a role in Pol I
CC recruitment (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- DOMAIN: Although it shares weak sequence similarity with GTF2B/TFIIB,
CC displays a similar subdomain organization as GTF2B/TFIIB, with a N-
CC terminal zinc finger, a connecting region (composed of B-reader and B-
CC linker regions), followed by 2 cyclin folds. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RRN7/TAF1B family. {ECO:0000305}.
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DR EMBL; HE601055; CAP33574.2; -; Genomic_DNA.
DR AlphaFoldDB; A8XLS0; -.
DR STRING; 6238.CBG15174; -.
DR PRIDE; A8XLS0; -.
DR WormBase; CBG15174; CBP38646; WBGene00035498; -.
DR eggNOG; ENOG502SDRV; Eukaryota.
DR HOGENOM; CLU_015505_0_0_1; -.
DR InParanoid; A8XLS0; -.
DR OMA; VAFCHSE; -.
DR OrthoDB; 562034at2759; -.
DR Proteomes; UP000008549; Chromosome III.
DR GO; GO:0070860; C:RNA polymerase I core factor complex; IBA:GO_Central.
DR GO; GO:0005668; C:RNA polymerase transcription factor SL1 complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0001164; F:RNA polymerase I core promoter sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0042790; P:nucleolar large rRNA transcription by RNA polymerase I; IBA:GO_Central.
DR GO; GO:0001188; P:RNA polymerase I preinitiation complex assembly; ISS:UniProtKB.
DR InterPro; IPR033599; TAF1B/Rrn7.
DR InterPro; IPR021752; TF_Rrn7_Zf.
DR PANTHER; PTHR31576; PTHR31576; 1.
DR Pfam; PF11781; zf-RRN7; 1.
PE 3: Inferred from homology;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..889
FT /note="TATA box-binding protein-associated factor RNA
FT polymerase I subunit B"
FT /id="PRO_0000416874"
FT ZN_FING 1..33
FT /note="RRN7-type"
FT REGION 35..101
FT /note="B-reader"
FT /evidence="ECO:0000250"
FT REGION 102..113
FT /note="B-linker"
FT /evidence="ECO:0000250"
FT REGION 114..351
FT /note="N-terminal cyclin fold"
FT /evidence="ECO:0000250"
FT REGION 229..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 352..510
FT /note="C-terminal cyclin fold"
FT /evidence="ECO:0000250"
FT COMPBIAS 230..244
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 9
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 12
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 26
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 29
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
SQ SEQUENCE 889 AA; 102809 MW; 0BB3DFEA6273D6AD CRC64;
MAPETNEKCK ACGGFNFSMI DGFKYCDRCG TLLENFEELE AEEGGIQQTR GAGKIKIKKK
GGDDGEKKTT NLVSTNESNV QIMRKALEKR SDFFSRQALK NDELAFPHES TPDYLYRLGL
RLAAFTQVLA KVGHVLVKEL NFEPRVLPTI LATFQRYLAH CHVAFCHSEQ CGSDEQLRFV
AMMENLEFEQ QEREEKRRKK LARRGKGVKA LSKSAAAWTL LTQGNITENL DLDSEEDEEE
EENPNLNKSM ENLEFEDTQN DETVAVNDTT VGFVRKITTA LSTEALRRAR QMILNLEILV
AIIHSALMSC GYRNVLASDV VRWIREDRFR ISLRSIRLMR HSAKEQETKE AMTKVDYAEP
YLRFPLYEIT RTSTLFHQSL NLNEKLVSLN FETLAARLCD NLNLPVEFLS RVLLLESMIP
CDVNPSLRKQ ADVSMGHNCE QLAAIQPKLY NSGFLSCFGR KERTWREADN CDESVKLALL
RIQCICRVLL SPDTKLMAYI LLVFRLTFDI DNATCSPDSK DALKFDIDTW IHQLEMRLKC
WQEHDMSMVL RSSCPVPDIQ ISTPFGPNYS YYDKKGNPWV HRLRRQVGFA KCIPSEMSFN
STSSLPTVFD IRQNRFKTER RQLEAVMSPL KFQRVILRKE MERDPEKYRN IVDPDSEKTF
FKDFTVRKVL ENTQKIAETS NSFDEYFPCA SRYTIYKRPD WIQNCTARSK QLSPKIGPYR
FYISNQACDD LLGVATSSFS PRFKFLLDSL ALIIGEDPKA LYTAFVMLEM HLTSSDTLET
IRESLLRSKP ITVKCQKFRK TMWHVECLRR VVTEHPVGKI EDLKYFIVAS TRESQEELAE
SSEAYLMHFR NHEIREDLTS LEAEKVQNRV LKLAYDFETF FGILAVKMW
