TAF1B_DROME
ID TAF1B_DROME Reviewed; 872 AA.
AC Q9VH20; Q494H4; Q8MRI0;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=TATA box-binding protein-associated factor RNA polymerase I subunit B;
DE AltName: Full=TATA box-binding protein-associated factor 1B;
DE Short=TBP-associated factor 1B;
GN Name=TAF1B {ECO:0000312|FlyBase:FBgn0037792};
GN ORFNames=CG6241 {ECO:0000312|FlyBase:FBgn0037792};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 72-872.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Component of RNA polymerase I core factor complex that acts
CC as a GTF2B/TFIIB-like factor and plays a key role in multiple steps
CC during transcription initiation such as pre-initiation complex (PIC)
CC assembly and postpolymerase recruitment events in polymerase I (Pol I)
CC transcription. Binds rDNA promoters and plays a role in Pol I
CC recruitment (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q9VH20; P20028: RpI135; NbExp=4; IntAct=EBI-104191, EBI-3403932;
CC Q9VH20; A1Z7A5: udd; NbExp=5; IntAct=EBI-104191, EBI-126425;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- DOMAIN: Although it shares weak sequence similarity with GTF2B/TFIIB,
CC displays a similar subdomain organization as GTF2B/TFIIB, with a N-
CC terminal zinc finger, a connecting region (composed of B-reader and B-
CC linker regions), followed by 2 cyclin folds. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RRN7/TAF1B family. {ECO:0000305}.
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DR EMBL; AE014297; AAF54500.1; -; Genomic_DNA.
DR EMBL; BT023802; AAZ66309.1; -; mRNA.
DR EMBL; BT057993; ACM16703.1; -; mRNA.
DR EMBL; AY119611; AAM50265.1; -; mRNA.
DR RefSeq; NP_001262451.1; NM_001275522.1.
DR RefSeq; NP_649982.1; NM_141725.3.
DR AlphaFoldDB; Q9VH20; -.
DR BioGRID; 66393; 5.
DR DIP; DIP-61426N; -.
DR IntAct; Q9VH20; 7.
DR STRING; 7227.FBpp0305959; -.
DR PaxDb; Q9VH20; -.
DR PRIDE; Q9VH20; -.
DR DNASU; 41242; -.
DR EnsemblMetazoa; FBtr0082185; FBpp0081663; FBgn0037792.
DR EnsemblMetazoa; FBtr0333825; FBpp0305959; FBgn0037792.
DR GeneID; 41242; -.
DR KEGG; dme:Dmel_CG6241; -.
DR UCSC; CG6241-RA; d. melanogaster.
DR CTD; 9014; -.
DR FlyBase; FBgn0037792; TAF1B.
DR VEuPathDB; VectorBase:FBgn0037792; -.
DR eggNOG; ENOG502S10D; Eukaryota.
DR GeneTree; ENSGT00440000033827; -.
DR HOGENOM; CLU_016145_0_0_1; -.
DR InParanoid; Q9VH20; -.
DR OMA; NMGAKPE; -.
DR OrthoDB; 329399at2759; -.
DR PhylomeDB; Q9VH20; -.
DR Reactome; R-DME-73772; RNA Polymerase I Promoter Escape.
DR SignaLink; Q9VH20; -.
DR BioGRID-ORCS; 41242; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41242; -.
DR PRO; PR:Q9VH20; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037792; Expressed in egg cell and 22 other tissues.
DR ExpressionAtlas; Q9VH20; baseline and differential.
DR Genevisible; Q9VH20; DM.
DR GO; GO:0005730; C:nucleolus; IDA:FlyBase.
DR GO; GO:0070860; C:RNA polymerase I core factor complex; IBA:GO_Central.
DR GO; GO:0005668; C:RNA polymerase transcription factor SL1 complex; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0001164; F:RNA polymerase I core promoter sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0042790; P:nucleolar large rRNA transcription by RNA polymerase I; IMP:FlyBase.
DR GO; GO:0001188; P:RNA polymerase I preinitiation complex assembly; ISS:UniProtKB.
DR InterPro; IPR033599; TAF1B/Rrn7.
DR InterPro; IPR021752; TF_Rrn7_Zf.
DR PANTHER; PTHR31576; PTHR31576; 1.
DR Pfam; PF11781; zf-RRN7; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..872
FT /note="TATA box-binding protein-associated factor RNA
FT polymerase I subunit B"
FT /id="PRO_0000416876"
FT ZN_FING 6..40
FT /note="RRN7-type"
FT REGION 41..72
FT /note="B-reader"
FT /evidence="ECO:0000250"
FT REGION 73..84
FT /note="B-linker"
FT /evidence="ECO:0000250"
FT REGION 85..312
FT /note="N-terminal cyclin fold"
FT /evidence="ECO:0000250"
FT REGION 187..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 15
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 18
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 32
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 35
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT CONFLICT 766
FT /note="P -> Q (in Ref. 3; AAZ66309)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 872 AA; 101775 MW; BDC8BB7440FEC5B4 CRC64;
MEEVLETMQL ENMHCDVCEG TTFQEREGFY YCVECGTQKD QIRAVDITAE DNFDDTAAGR
YTARTIRQKK DTEKEDEDDI TSWEFYNYVL RGFLQELLNM GAKPELKLMT LQVWAAYLDS
MEVAFSKSNK TGLPKLNVRA LPIDARIIYN HKTFKKGKKG KKSTLTGDPN DERAKFRLWN
RTKRNLDASG YRSHGGASES EGEQSLHLQW SMRARKSLKR HMPLKHLDKH SRDSKGSMSC
HSLRPRVKQL HNFDRNIYCL NIIKLYVVLG IALNMVEDDI QLSDLLRFID EEHLTKRCML
NYLPGNVAAK GKALLKDMEL SKMKDKVTNK LLRVNIACMS RFINLSEYQK PNLHSLAERY
ILELALPPRL LKYVNSLLDL HPPTFFNAMT VHPYPRYEAR TMAYILYAMK LLFGLDDLKE
RNISESAAKI NEKLLEVGGD EAPLLFVFTE WMEFVEMRKV IVSHYNQSFA RRFGVSTRTG
CQVDDILAKE WKEKEQGETF GWMQGSAAMK RQHENLTHII ETMLKDHFGE SSKESMEKEH
IEFQPSLTPA HSYFNRILLQ VSRSDGAKMK ITIPDHMKVD HSARNLDPFV LETTELSQYL
SQHGLKLRVE ELACQEDIQN VGIFRPLTII RGDGREYRAN TEIKTETWIS ELKRKEKRPD
FRFTQPTGTY GARYLKRITM RDARRVQLEI NNPFWDVTET PSFLLKLNDN EIPLDSLSSL
QTFEEGTMDP LNIPLDLPRR NLEKILNPEG SDRASDQVAS DINDEPPSPE TLLLQVSNFD
CWLLHGYMKR IRRHDKHELR QLFPCSFRWL LETCASTIGI VWEELYEELL IVEVMYHHSI
RDWSNHRNYL CIQHNTQEKD IRTLARTYKE LW
