TAF1_CAEEL
ID TAF1_CAEEL Reviewed; 1744 AA.
AC G5EGM3;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Transcription initiation factor TFIID subunit 1 {ECO:0000250|UniProtKB:P21675};
DE AltName: Full=TBP-associated transcription factor 1 {ECO:0000312|WormBase:W04A8.7};
GN Name=taf-1 {ECO:0000312|WormBase:W04A8.7};
GN ORFNames=W04A8.7 {ECO:0000312|WormBase:W04A8.7};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP NOMENCLATURE.
RX PubMed=11963920; DOI=10.1101/gad.976402;
RA Tora L.;
RT "A unified nomenclature for TATA box binding protein (TBP)-associated
RT factors (TAFs) involved in RNA polymerase II transcription.";
RL Genes Dev. 16:673-675(2002).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=14726532; DOI=10.1074/jbc.m310731200;
RA Walker A.K., Shi Y., Blackwell T.K.;
RT "An extensive requirement for transcription factor IID-specific TAF-1 in
RT Caenorhabditis elegans embryonic transcription.";
RL J. Biol. Chem. 279:15339-15347(2004).
CC -!- FUNCTION: The TFIID basal transcription factor complex plays a major
CC role in the initiation of RNA polymerase II (Pol II)-dependent
CC transcription (By similarity). TFIID recognizes and binds promoters via
CC its subunit tbp-1, a TATA-box-binding protein, and promotes assembly of
CC the pre-initiation complex (PIC) (By similarity). The TFIID complex
CC consists of tbp-1 and TBP-associated factors (TAFs), including taf-1
CC (By similarity). May regulate RNA polymerase II activity and thereby
CC may control transcription initiation by RNA polymerase II
CC (PubMed:14726532). Required for early embryonic development
CC (PubMed:14726532). Essential for embryonic transcription of several
CC genes (PubMed:14726532). {ECO:0000250|UniProtKB:P21675,
CC ECO:0000269|PubMed:14726532}.
CC -!- SUBUNIT: Component of the TFIID basal transcription factor complex,
CC composed of TATA-box-binding protein tbp-1, and a number of TBP-
CC associated factors (TAFs). {ECO:0000250|UniProtKB:P21675}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14726532}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryo.
CC {ECO:0000269|PubMed:14726532}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes embryonic arrest
CC at the 100-cell stage, premature cell division of E2 cells, Ea and Ep,
CC severe loss of polymerase II large subunit ama-1 phosphorylation and
CC reduction in the transcription of several embryonic genes.
CC {ECO:0000269|PubMed:14726532}.
CC -!- SIMILARITY: Belongs to the TAF1 family. {ECO:0000305}.
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DR EMBL; BX284601; CAB04907.3; -; Genomic_DNA.
DR RefSeq; NP_493426.2; NM_061025.4.
DR AlphaFoldDB; G5EGM3; -.
DR SMR; G5EGM3; -.
DR IntAct; G5EGM3; 1.
DR STRING; 6239.W04A8.7; -.
DR EPD; G5EGM3; -.
DR PaxDb; G5EGM3; -.
DR PeptideAtlas; G5EGM3; -.
DR EnsemblMetazoa; W04A8.7.1; W04A8.7.1; WBGene00006382.
DR GeneID; 173257; -.
DR KEGG; cel:CELE_W04A8.7; -.
DR CTD; 173257; -.
DR WormBase; W04A8.7; CE42634; WBGene00006382; taf-1.
DR eggNOG; KOG0008; Eukaryota.
DR GeneTree; ENSGT00940000155242; -.
DR HOGENOM; CLU_000572_3_1_1; -.
DR InParanoid; G5EGM3; -.
DR OMA; DSMAMQM; -.
DR OrthoDB; 103411at2759; -.
DR PhylomeDB; G5EGM3; -.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-CEL-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-CEL-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-CEL-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR PRO; PR:G5EGM3; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00006382; Expressed in embryo and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0005669; C:transcription factor TFIID complex; ISS:WormBase.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004402; F:histone acetyltransferase activity; ISS:WormBase.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; ISS:WormBase.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0001091; F:RNA polymerase II general transcription initiation factor binding; IEA:InterPro.
DR GO; GO:0017025; F:TBP-class protein binding; IBA:GO_Central.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IBA:GO_Central.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IMP:WormBase.
DR Gene3D; 1.20.920.10; -; 2.
DR InterPro; IPR001487; Bromodomain.
DR InterPro; IPR036427; Bromodomain-like_sf.
DR InterPro; IPR018359; Bromodomain_CS.
DR InterPro; IPR040240; TAF1.
DR InterPro; IPR011177; TAF1_animal.
DR InterPro; IPR022591; TAF1_HAT_dom.
DR InterPro; IPR041670; Znf-CCHC_6.
DR PANTHER; PTHR13900; PTHR13900; 2.
DR Pfam; PF00439; Bromodomain; 2.
DR Pfam; PF12157; DUF3591; 1.
DR Pfam; PF15288; zf-CCHC_6; 1.
DR PIRSF; PIRSF003047; TAF1_animal; 1.
DR PRINTS; PR00503; BROMODOMAIN.
DR SMART; SM00297; BROMO; 2.
DR SUPFAM; SSF47370; SSF47370; 2.
DR PROSITE; PS00633; BROMODOMAIN_1; 1.
DR PROSITE; PS50014; BROMODOMAIN_2; 2.
PE 2: Evidence at transcript level;
KW Bromodomain; Coiled coil; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..1744
FT /note="Transcription initiation factor TFIID subunit 1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000435363"
FT DOMAIN 1425..1495
FT /note="Bromo 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00035"
FT DOMAIN 1547..1617
FT /note="Bromo 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00035"
FT REGION 1..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 248..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 429..488
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1001..1024
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1071..1098
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1186..1213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1319..1391
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1666..1702
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1714..1744
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1019..1080
FT /evidence="ECO:0000255"
FT COILED 1161..1204
FT /evidence="ECO:0000255"
FT COILED 1282..1314
FT /evidence="ECO:0000255"
FT MOTIF 1379..1386
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000305"
FT COMPBIAS 8..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 439..453
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1071..1096
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1186..1206
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1371..1385
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1670..1695
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1744 AA; 198522 MW; F3EBA6DE00F5937A CRC64;
MNNTHHHTNG YRTEKPKNEE DLEDPYANLS DFYKNHPAAR NEACSSASNG GSKSVKMEPK
VEKNEEFEEY IGDPVRLEDM EPFARPSLRD DAPLSSILHP DLDGIDPRIF FKDFNPNKTL
RFSRLFAQNI KHTSRAEIWW ASRTFSKHQR KKEPEEPLAD DVIVGAKKLK LNIIEKVPRV
MLADDEEERM RRPILTDAEE MAKKNEEGTV VQPWRTGPAK IWYDMMNLPM TSQAVNYGFK
LKKSPQKVSI RSGKPLNYRT PDDLPSTSSG PAPNSAPFLD KVEVIDKSCE ASTSEDILLP
YQVIEWENDV ILDGEEVKDQ LLEEFSNGRG CGWIPTQYTR TYEHFVYAAN NNAFEQMFDG
KSAPINLTGP DSAILPTPGH SIFPSAPCDL DILPWETNII WDADAMPSTL EPIDFLVDFQ
DDPLIYGMPE DRRHDEGPDH HHHHHHHRKD GQYTKKSKMI LGQVQQRQKQ EEDEQMESTM
AQFTDNDPFN LSNDDYYVPK ATSKTLSNNS LLIQHSTPAT NIATHFFPTH PSAFRLRYWH
RTPFTRRIVR HWQPMRFQPI QTPVKHQQRV AAMREAMRQA QGGGEVFYMR DVQDLSGKDE
TLVMIEYSEE HPVILSQPGM ASKMKNYFKR RQANDSEPTF TFGELAFSHQ IPFLGQLQPG
QSLQSIENML YRAPIYLHKR QNTDFLLIRS MNQWYIRPLP SIFVAGQQCP LYEVPSPNSK
RATVFVRDFL FAFIYRLFWA SDSSPRRLKM DDVRNAFPHY AESNIRKRLK MCSTFVRQGS
ETYWSLKPDF RLPSKEEVLS MVTPEMCCAQ YSMMAAEQRL KDAGYGEKYF FTPENDEGSE
DEVTIEDEIK CAPWNTTRAF LASQREKCLL DQTGIADPTG CGQGFSYVRV SQKPHKDENA
TPVPKKLVTG TNADLRKLPL KEAKQICRGY GVKEEEISAL TRWEIIDVIR TLSTQAAKAT
KDGEIIAVSG MARFARGNTR FSSADMQEKY RKHCQRIFDQ QNQTLANTDP ISTDDDSTDA
DSDNEELASR LESMLEANKG KKNISMSEKA KIDFETEEKE REDLKRMIHG TTNQVEKGEK
KEEGEVTAEE KKSASQFGED VAMSASKISG ITANQQLKIY RTCKGPDGKD VTRIEIVTRP
QLIEAYTRIR MTRDDTFIQV YAQMDEQYKE EKRKKKRRLQ DQIRRMKKNE EKAAHKVQKM
TEKKVKPIKP PNPNLQKMRC SACHAYGHMK TNRNCPLYGK DPLTPLKEED EGSTIMTSVS
SASLVAPDAV QVDGTKVKFN LNFAEIRKEQ NREEKLKRKL AKMAEAAVRE RQMAHLMEYG
GGASSSGGAG GGGSGIGGST GGGITDNDDD DRFSQISGTS SFLNGPPGAI RGGNRNSSVS
GSKRRSSMMP EEDYLQGPLK VAHRARADPK VVMSSMLTDI VNELKMISGS DAFVTPVNSK
KVVDYYNIIK NPISLQEIKK KISEQSYLLR KDFLDDIKLM FDNSRMYNGD NNILTLTAQQ
MLQLAGKRMI EREQKFIGLE KQINPLLDTN DLIGFSYLLG EIVQKMKNIP KSALFHTRVD
PKKIPAYYLK ISDPMDLSIM EQKSKSQEYK SIDEFLKDAE KIYTNSVVFN GAESVYSLKA
KEMFEMAEML VKDQMDTLGE LERNINPSAI NDAAAAQRGL AMDSDDHMDE MEDHPTEEEE
EDDDDEIMDD DMDIDATGYS YDHDDNVAVG QIFNDLAMSD SDEDERAEDV KRPANGDDNL
LDSF
