TAF4B_ARATH
ID TAF4B_ARATH Reviewed; 852 AA.
AC F4K4L7; Q6SJR0; Q9FMH0;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Transcription initiation factor TFIID subunit 4b;
DE AltName: Full=TBP-associated factor 4b;
DE Short=AtTAF4b;
GN Name=TAF4B; OrderedLocusNames=At5g43130; ORFNames=AAF24960.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, NOMENCLATURE, AND TISSUE
RP SPECIFICITY.
RX PubMed=15527982; DOI=10.1016/j.gene.2004.08.023;
RA Lago C., Clerici E., Mizzi L., Colombo L., Kater M.M.;
RT "TBP-associated factors in Arabidopsis.";
RL Gene 342:231-241(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBUNIT, AND INTERACTION WITH TAF5;
RP TAF8; TAF9; TAF10; TAF12; TAF12B; TAF13; TAF14; TAF14B; TAF15 AND TAF15B.
RX PubMed=17340043; DOI=10.1007/s11103-007-9135-1;
RA Lawit S.J., O'Grady K., Gurley W.B., Czarnecka-Verner E.;
RT "Yeast two-hybrid map of Arabidopsis TFIID.";
RL Plant Mol. Biol. 64:73-87(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: TAFs are components of the transcription factor IID (TFIID)
CC complex that is essential for mediating regulation of RNA polymerase
CC transcription. {ECO:0000250}.
CC -!- SUBUNIT: Component of the TFIID complex. TFIID is composed of TATA
CC binding protein (TBP) and a number of TBP-associated factors (TAFs)
CC whose MWs range from 14-217 kDa. Can homodimerize. Interacts with TAF5,
CC TAF8, TAF9, TAF10, TAF12, TAF12B, TAF13 TAF14, TAF14B, TAF15 and
CC TAF15B. {ECO:0000269|PubMed:17340043}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4K4L7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4K4L7-2; Sequence=VSP_053300;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves and inflorescences.
CC {ECO:0000269|PubMed:15527982}.
CC -!- SIMILARITY: Belongs to the TAF4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08277.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY457044; AAR21620.1; -; mRNA.
DR EMBL; AB008267; BAB08277.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94913.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94914.1; -; Genomic_DNA.
DR EMBL; AK229454; BAF01312.1; -; mRNA.
DR RefSeq; NP_001190458.1; NM_001203529.1. [F4K4L7-1]
DR RefSeq; NP_199127.3; NM_123679.4. [F4K4L7-2]
DR PDB; 7AC1; NMR; -; A=182-254.
DR PDBsum; 7AC1; -.
DR AlphaFoldDB; F4K4L7; -.
DR SMR; F4K4L7; -.
DR BioGRID; 19580; 15.
DR IntAct; F4K4L7; 14.
DR STRING; 3702.AT5G43130.2; -.
DR iPTMnet; F4K4L7; -.
DR PaxDb; F4K4L7; -.
DR PRIDE; F4K4L7; -.
DR ProteomicsDB; 234132; -. [F4K4L7-1]
DR EnsemblPlants; AT5G43130.1; AT5G43130.1; AT5G43130. [F4K4L7-2]
DR EnsemblPlants; AT5G43130.2; AT5G43130.2; AT5G43130. [F4K4L7-1]
DR GeneID; 834330; -.
DR Gramene; AT5G43130.1; AT5G43130.1; AT5G43130. [F4K4L7-2]
DR Gramene; AT5G43130.2; AT5G43130.2; AT5G43130. [F4K4L7-1]
DR KEGG; ath:AT5G43130; -.
DR Araport; AT5G43130; -.
DR TAIR; locus:2167806; AT5G43130.
DR eggNOG; KOG2341; Eukaryota.
DR InParanoid; F4K4L7; -.
DR OMA; ERQSDSH; -.
DR OrthoDB; 409323at2759; -.
DR PRO; PR:F4K4L7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4K4L7; baseline and differential.
DR Genevisible; F4K4L7; AT.
DR GO; GO:0005669; C:transcription factor TFIID complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd08045; TAF4; 1.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR022003; RST.
DR InterPro; IPR045144; TAF4.
DR InterPro; IPR007900; TAF4_C.
DR PANTHER; PTHR15138; PTHR15138; 2.
DR Pfam; PF12174; RST; 1.
DR Pfam; PF05236; TAF4; 1.
DR PROSITE; PS51879; RST; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative splicing; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..852
FT /note="Transcription initiation factor TFIID subunit 4b"
FT /id="PRO_0000424041"
FT DOMAIN 184..255
FT /note="RST"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01227"
FT REGION 41..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 284..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 354..528
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 662..712
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 742..777
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..142
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 362..380
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 386..442
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 450..488
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 746..763
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 777..805
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17340043, ECO:0000303|Ref.5"
FT /id="VSP_053300"
FT HELIX 193..203
FT /evidence="ECO:0007829|PDB:7AC1"
FT HELIX 206..220
FT /evidence="ECO:0007829|PDB:7AC1"
FT HELIX 226..237
FT /evidence="ECO:0007829|PDB:7AC1"
FT HELIX 239..250
FT /evidence="ECO:0007829|PDB:7AC1"
SQ SEQUENCE 852 AA; 93741 MW; 7F1D7D6AA0CB16C9 CRC64;
MDPSIFKLLE EDEDESMHSG ADVDAFQAAL NRDIEGSMTT SIPHVTNPGN NHSSRQQFST
WKNGIGDSNI NVQTQHSLES TQMKEQEGST LENQHQHDLK RANEPHLQHN QPQDLHRAGQ
LWENPSQVPQ STGLPISEKN PTGNESDRSH NQESESQYMK LQKMSSQQAR GVEPPVNPMN
VNPINRNPKQ VPFAALLPTL MNQLDKDRAL QLRTLYARLK KNEIPKEGFT RHMKDIVGDQ
MLRMAVSKLQ QVNYNQGKIG IQAPSTEINN QKSQSDPRAV HLNQLPSSAS GTLGSSVPVQ
GLTKHPQHQM QHPPSSFPMY TTSGSFHSFP GPNTNASGST LRPHLHDSHM RHVAHNQPMG
STGLGGPPQS TTNMMTMPKF ERPSSVNDPS RVQGGATSHF QNSSSLPLNS APGQGSSVSH
VKQESVDQSF EKNNAASMTS NEDLEKESSR MVLSTPNNMA PASSVSPSMT TQLDASTTMN
SRGPLGTSQG GANARMPPKK PSVGQKKPLE TLGSSPPPPS KKQKVAGNSM DQSIEQLNDV
TAVSGVNLRE EEEQLFSGAK EDGRVSEASR RVVHEEEERL ILQKNPLQRK LAEIMAKAGL
KQISNDVERC LSLCVEERMR GLLSHIIRLS KQRVDAEKSR HRTFITSDIR LQINEMNQKV
KEEWEKKQAE AEKLKKPSES EEGDGGVDSE KDKEDNRSKG VKGNKEDDDK MRTTAANVAA
RAAVGGDDAF LKWQLMAEAR QKSVSEAGKD GNQKTTSGGG KNSKDRQDGG RRFSGTESSC
GVGIVYRVSS SRFWFAMMSF GFLFAGGRRV GKNQGSSLQP KVVRTISVKD VVAVLEREPQ
MSKSTLMYRL IQ
