TAF4B_HUMAN
ID TAF4B_HUMAN Reviewed; 862 AA.
AC Q92750; Q29YA4; Q29YA5;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 2.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=Transcription initiation factor TFIID subunit 4B;
DE AltName: Full=Transcription initiation factor TFIID 105 kDa subunit;
DE Short=TAF(II)105;
DE Short=TAFII-105;
DE Short=TAFII105;
GN Name=TAF4B; Synonyms=TAF2C2, TAFII105;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC TISSUE=Ovarian carcinoma;
RX PubMed=16088961; DOI=10.1002/jcb.20577;
RA Wu Y., Lu Y., Hu Y., Li R.;
RT "Cyclic AMP-dependent modification of gonad-selective TAF(II)105 in a human
RT ovarian granulosa cell line.";
RL J. Cell. Biochem. 96:751-759(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 67-862 (ISOFORM 1), PROTEIN SEQUENCE OF
RP 81-85; 242-253; 281-290; 302-310; 377-388; 473-479; 507-522; 580-587;
RP 675-689; 697-712 AND 746-800, AND TISSUE SPECIFICITY.
RX PubMed=8858156; DOI=10.1016/s0092-8674(00)81330-6;
RA Dikstein R., Zhou S., Tjian R.;
RT "Human TAFII 105 is a cell type-specific TFIID subunit related to
RT hTAFII130.";
RL Cell 87:137-146(1996).
RN [3]
RP INTERACTION WITH POU2AF1, AND FUNCTION.
RX PubMed=10828057; DOI=10.1074/jbc.275.22.16459;
RA Wolstein O., Silkov A., Revach M., Dikstein R.;
RT "Specific interaction of TAFII105 with OCA-B is involved in activation of
RT octamer-dependent transcription.";
RL J. Biol. Chem. 275:16459-16465(2000).
RN [4]
RP INTERACTION WITH P65/RELA AND REL, AND FUNCTION.
RX PubMed=10849440; DOI=10.1074/jbc.275.24.18180;
RA Yamit-Hezi A., Nir S., Wolstein O., Dikstein R.;
RT "Interaction of TAFII105 with selected p65/RelA dimers is associated with
RT activation of subset of NF-kappa B genes.";
RL J. Biol. Chem. 275:18180-18187(2000).
RN [5]
RP SUBCELLULAR LOCATION, AND NUCLEAR EXPORT SIGNAL.
RX PubMed=11567023; DOI=10.1074/jbc.m106112200;
RA Rashevsky-Finkel A., Silkov A., Dikstein R.;
RT "A composite nuclear export signal in the TBP-associated factor TAFII105.";
RL J. Biol. Chem. 276:44963-44969(2001).
RN [6]
RP INTERACTION WITH TAF12 IN A COMPLEX WITH TAF6; TAF9; TAF12 AND TAF4B, AND
RP DNA-BINDING.
RX PubMed=15601843; DOI=10.1128/mcb.25.1.206-219.2005;
RA Shao H., Revach M., Moshonov S., Tzuman Y., Gazit K., Albeck S., Unger T.,
RA Dikstein R.;
RT "Core promoter binding by histone-like TAF complexes.";
RL Mol. Cell. Biol. 25:206-219(2005).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [9]
RP INVOLVEMENT IN SPGF13, AND POSSIBLE FUNCTION.
RX PubMed=24431330; DOI=10.1136/jmedgenet-2013-102102;
RA Ayhan O., Balkan M., Guven A., Hazan R., Atar M., Tok A., Tolun A.;
RT "Truncating mutations in TAF4B and ZMYND15 causing recessive azoospermia.";
RL J. Med. Genet. 51:239-244(2014).
CC -!- FUNCTION: Cell type-specific subunit of the general transcription
CC factor TFIID that may function as a gene-selective coactivator in
CC certain cells. TFIID is a multimeric protein complex that plays a
CC central role in mediating promoter responses to various activators and
CC repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-
CC kappa-B subunit. Involved in the activation of a subset of
CC antiapoptotic genes including TNFAIP3. May be involved in regulating
CC folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a
CC coactivator of B-cell-specific transcription. Plays a role in
CC spermiogenesis and oogenesis. {ECO:0000250|UniProtKB:G5E8Z2,
CC ECO:0000269|PubMed:10828057, ECO:0000269|PubMed:10849440,
CC ECO:0000269|PubMed:16088961, ECO:0000303|PubMed:24431330}.
CC -!- SUBUNIT: TFIID is composed of TATA binding protein (TBP) and a number
CC of TBP-associated factors (TAFs) (PubMed:15601843). Heterodimerizes
CC with TAF12/TFII20 via the C-terminal H2A-like histone-fold domain
CC (PubMed:15601843). This heterodimer forms a histone-like octamer with
CC the TAF6/TAFII70-TAF9/TAFII31 heterodimer (PubMed:15601843). Interacts
CC with P65/RELA homodimers and P65/RELA-REL heterodimers
CC (PubMed:10849440). Interaction with POU2AF1, via its C-terminal
CC activation domain, is required for octamer-dependent transcription
CC (PubMed:10828057). Interacts with ZNF628 (By similarity).
CC {ECO:0000250|UniProtKB:G5E8Z2, ECO:0000269|PubMed:10828057,
CC ECO:0000269|PubMed:10849440, ECO:0000269|PubMed:15601843}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11567023,
CC ECO:0000269|PubMed:16088961}. Cytoplasm {ECO:0000269|PubMed:11567023,
CC ECO:0000269|PubMed:16088961}. Note=Export into the cytoplasm is
CC mediated by a CRM1-independent nuclear export pathway and not by
CC phosphorylation. {ECO:0000269|PubMed:11567023}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q92750-1; Sequence=Displayed;
CC Name=2; Synonyms=TAFII105-E9;
CC IsoId=Q92750-2; Sequence=VSP_022025, VSP_022026;
CC -!- TISSUE SPECIFICITY: Preferentially expressed in ovarian granulosa cells
CC (at protein level). Highly expressed in B-cells.
CC {ECO:0000269|PubMed:16088961, ECO:0000269|PubMed:8858156}.
CC -!- PTM: Under stimulation by forskolin, Isoform 1 is phosphorylated by
CC protein kinase A (PKA). {ECO:0000269|PubMed:16088961}.
CC -!- DISEASE: Spermatogenic failure 13 (SPGF13) [MIM:615841]: A disorder
CC resulting in the absence (azoospermia) or reduction (oligozoospermia)
CC of sperm in the semen, leading to male infertility.
CC {ECO:0000269|PubMed:24431330}. Note=The disease is caused by variants
CC affecting the gene represented in this entry.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to exon inclusion.
CC Unphosphorylated form. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TAF4 family. {ECO:0000305}.
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DR EMBL; AY888048; AAX14897.1; -; mRNA.
DR EMBL; AY888049; AAX14898.1; -; mRNA.
DR EMBL; Y09321; CAA70499.1; -; mRNA.
DR CCDS; CCDS42421.1; -. [Q92750-1]
DR RefSeq; NP_001280654.1; NM_001293725.1.
DR RefSeq; NP_005631.1; NM_005640.2. [Q92750-1]
DR AlphaFoldDB; Q92750; -.
DR SMR; Q92750; -.
DR BioGRID; 112738; 23.
DR ComplexPortal; CPX-930; General transcription factor complex TFIID, TAF4B variant.
DR CORUM; Q92750; -.
DR IntAct; Q92750; 7.
DR STRING; 9606.ENSP00000269142; -.
DR GlyGen; Q92750; 7 sites, 1 O-linked glycan (7 sites).
DR iPTMnet; Q92750; -.
DR PhosphoSitePlus; Q92750; -.
DR BioMuta; TAF4B; -.
DR DMDM; 119370533; -.
DR EPD; Q92750; -.
DR jPOST; Q92750; -.
DR MassIVE; Q92750; -.
DR MaxQB; Q92750; -.
DR PaxDb; Q92750; -.
DR PeptideAtlas; Q92750; -.
DR PRIDE; Q92750; -.
DR ProteomicsDB; 75440; -. [Q92750-1]
DR ProteomicsDB; 75441; -. [Q92750-2]
DR Antibodypedia; 5168; 111 antibodies from 22 providers.
DR DNASU; 6875; -.
DR Ensembl; ENST00000269142.10; ENSP00000269142.6; ENSG00000141384.13. [Q92750-1]
DR Ensembl; ENST00000418698.3; ENSP00000389365.3; ENSG00000141384.13. [Q92750-2]
DR GeneID; 6875; -.
DR KEGG; hsa:6875; -.
DR MANE-Select; ENST00000269142.10; ENSP00000269142.6; NM_005640.3; NP_005631.1.
DR UCSC; uc002kvu.5; human. [Q92750-1]
DR CTD; 6875; -.
DR DisGeNET; 6875; -.
DR GeneCards; TAF4B; -.
DR HGNC; HGNC:11538; TAF4B.
DR HPA; ENSG00000141384; Low tissue specificity.
DR MalaCards; TAF4B; -.
DR MIM; 601689; gene.
DR MIM; 615841; phenotype.
DR neXtProt; NX_Q92750; -.
DR OpenTargets; ENSG00000141384; -.
DR Orphanet; 399805; Male infertility with azoospermia or oligozoospermia due to single gene mutation.
DR PharmGKB; PA36313; -.
DR VEuPathDB; HostDB:ENSG00000141384; -.
DR eggNOG; KOG2341; Eukaryota.
DR GeneTree; ENSGT00390000011620; -.
DR HOGENOM; CLU_010576_2_0_1; -.
DR InParanoid; Q92750; -.
DR OMA; HDIMEPN; -.
DR OrthoDB; 735381at2759; -.
DR PhylomeDB; Q92750; -.
DR TreeFam; TF316520; -.
DR PathwayCommons; Q92750; -.
DR Reactome; R-HSA-167161; HIV Transcription Initiation.
DR Reactome; R-HSA-167162; RNA Polymerase II HIV Promoter Escape.
DR Reactome; R-HSA-167172; Transcription of the HIV genome.
DR Reactome; R-HSA-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-HSA-6804756; Regulation of TP53 Activity through Phosphorylation.
DR Reactome; R-HSA-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-HSA-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-HSA-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-HSA-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR SignaLink; Q92750; -.
DR BioGRID-ORCS; 6875; 18 hits in 1081 CRISPR screens.
DR ChiTaRS; TAF4B; human.
DR GeneWiki; TAF4B; -.
DR GenomeRNAi; 6875; -.
DR Pharos; Q92750; Tbio.
DR PRO; PR:Q92750; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; Q92750; protein.
DR Bgee; ENSG00000141384; Expressed in secondary oocyte and 162 other tissues.
DR ExpressionAtlas; Q92750; baseline and differential.
DR Genevisible; Q92750; HS.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0001650; C:fibrillar center; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005669; C:transcription factor TFIID complex; IDA:ARUK-UCL.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0051059; F:NF-kappaB binding; IPI:UniProtKB.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IC:ARUK-UCL.
DR GO; GO:0042789; P:mRNA transcription by RNA polymerase II; IDA:ComplexPortal.
DR GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IDA:ComplexPortal.
DR GO; GO:0006468; P:protein phosphorylation; IDA:ComplexPortal.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd08045; TAF4; 1.
DR Gene3D; 1.10.20.10; -; 1.
DR Gene3D; 1.20.120.1110; -; 1.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR045144; TAF4.
DR InterPro; IPR007900; TAF4_C.
DR InterPro; IPR037249; TAFH/NHR1_dom_sf.
DR InterPro; IPR003894; TAFH_NHR1.
DR PANTHER; PTHR15138; PTHR15138; 1.
DR Pfam; PF05236; TAF4; 1.
DR Pfam; PF07531; TAFH; 1.
DR SMART; SM00549; TAFH; 1.
DR SUPFAM; SSF158553; SSF158553; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS51119; TAFH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Differentiation;
KW Direct protein sequencing; DNA-binding; Nucleus; Oogenesis; Phosphoprotein;
KW Reference proteome; Spermatogenesis; Transcription;
KW Transcription regulation.
FT CHAIN 1..862
FT /note="Transcription initiation factor TFIID subunit 4B"
FT /id="PRO_0000118872"
FT DOMAIN 256..353
FT /note="TAFH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00440"
FT DOMAIN 653..702
FT /note="Histone-fold"
FT REGION 100..241
FT /note="Sufficient for interaction with ZNF628"
FT /evidence="ECO:0000250|UniProtKB:G5E8Z2"
FT REGION 219..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 511..533
FT /note="Required for interaction with P65/RELA"
FT /evidence="ECO:0000269|PubMed:10849440"
FT REGION 830..862
FT /note="Required for interaction with TAF12"
FT /evidence="ECO:0000269|PubMed:15601843"
FT COILED 722..787
FT /evidence="ECO:0000255"
FT MOTIF 516..556
FT /note="Nuclear export signal"
FT MOD_RES 595
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 612..618
FT /note="DEDDIND -> STFICVY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16088961"
FT /id="VSP_022025"
FT VAR_SEQ 619..862
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16088961"
FT /id="VSP_022026"
FT VARIANT 249
FT /note="I -> V (in dbSNP:rs16942219)"
FT /id="VAR_052259"
FT VARIANT 539
FT /note="N -> S (in dbSNP:rs12963653)"
FT /id="VAR_061836"
FT CONFLICT 490..496
FT /note="SAGTTSD -> FCWDHIC (in Ref. 2; CAA70499)"
FT /evidence="ECO:0000305"
FT CONFLICT 523..530
FT /note="QAVQVKQL -> TGSSSKQLFSLFH (in Ref. 2; CAA70499)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 862 AA; 91091 MW; E380D5CD2445AC11 CRC64;
MPAGLTEPAG AAPPAAVSAS GTVTMAPAGA LPVRVESTPV ALGAVTKAPV SVCVEPTASQ
PLRSPVGTLV TKVAPVSAPP KVSSGPRLPA PQIVAVKAPN TTTIQFPANL QLPPGTVLIK
SNSGPLMLVS PQQTVTRAET TSNITSRPAV PANPQTVKIC TVPNSSSQLI KKVAVTPVKK
LAQIGTTVVT TVPKPSSVQS VAVPTSVVTV TPGKPLNTVT TLKPSSLGAS STPSNEPNLK
AENSAAVQIN LSPTMLENVK KCKNFLAMLI KLACSGSQSP EMGQNVKKLV EQLLDAKIEA
EEFTRKLYVE LKSSPQPHLV PFLKKSVVAL RQLLPNSQSF IQQCVQQTSS DMVIATCTTT
VTTSPVVTTT VSSSQSEKSI IVSGATAPRT VSVQTLNPLA GPVGAKAGVV TLHSVGPTAA
TGGTTAGTGL LQTSKPLVTS VANTVTTVSL QPEKPVVSGT AVTLSLPAVT FGETSGAAIC
LPSVKPVVSS AGTTSDKPVI GTPVQIKLAQ PGPVLSQPAG IPQAVQVKQL VVQQPSGGNE
KQVTTISHSS TLTIQKCGQK TMPVNTIIPT SQFPPASILK QITLPGNKIL SLQASPTQKN
RIKENVTSCF RDEDDINDVT SMAGVNLNEE NACILATNSE LVGTLIQSCK DEPFLFIGAL
QKRILDIGKK HDITELNSDA VNLISQATQE RLRGLLEKLT AIAQHRMTTY KASENYILCS
DTRSQLKFLE KLDQLEKQRK DLEEREMLLK AAKSRSNKED PEQLRLKQKA KELQQLELAQ
IQHRDANLTA LAAIGPRKKR PLESGIEGLK DNLLASGTSS LTATKQLHRP RITRICLRDL
IFCMEQEREM KYSRALYLAL LK
