TAF4B_MOUSE
ID TAF4B_MOUSE Reviewed; 850 AA.
AC G5E8Z2;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2018, sequence version 2.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Transcription initiation factor TFIID subunit 4B;
DE AltName: Full=Transcription initiation factor TFIID 105 kDa subunit;
DE Short=TAF(II)105;
DE Short=TAFII-105;
DE Short=TAFII105;
GN Name=Taf4b; Synonyms=Taf2c2, Tafii105;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 513-815.
RG The MGC Project Team;
RL Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DISRUPTION PHENOTYPE, FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11557891; DOI=10.1126/science.1061935;
RA Freiman R.N., Albright S.R., Zheng S., Sha W.C., Hammer R.E., Tjian R.;
RT "Requirement of tissue-selective TBP-associated factor TAFII105 in ovarian
RT development.";
RL Science 293:2084-2087(2001).
RN [5]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=15774719; DOI=10.1101/gad.1290105;
RA Falender A.E., Freiman R.N., Geles K.G., Lo K.C., Hwang K., Lamb D.J.,
RA Morris P.L., Tjian R., Richards J.S.;
RT "Maintenance of spermatogenesis requires TAF4b, a gonad-specific subunit of
RT TFIID.";
RL Genes Dev. 19:794-803(2005).
RN [6]
RP INTERACTION WITH ZNF628.
RX PubMed=31932482; DOI=10.1128/mcb.00228-19;
RA Gustafson E.A., Seymour K.A., Sigrist K., Rooij D.G.D.E., Freiman R.N.;
RT "ZFP628 Is a TAF4b-Interacting Transcription Factor Required for Mouse
RT Spermiogenesis.";
RL Mol. Cell. Biol. 40:0-0(2020).
CC -!- FUNCTION: Cell type-specific subunit of the general transcription
CC factor TFIID that may function as a gene-selective coactivator in
CC certain cells. TFIID is a multimeric protein complex that plays a
CC central role in mediating promoter responses to various activators
CC asond repressors. TAF4B is a transcriptional coactivator of the
CC p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of
CC antiapoptotic genes including TNFAIP3. Through interaction with
CC OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription.
CC Plays a role in spermiogenesis and oogenesis.
CC {ECO:0000250|UniProtKB:Q92750, ECO:0000269|PubMed:11557891,
CC ECO:0000269|PubMed:15774719}.
CC -!- SUBUNIT: TFIID is composed of TATA binding protein (TBP) and a number
CC of TBP-associated factors (TAFs). Heterodimerizes with TAF12/TFII20 via
CC the C-terminal H2A-like histone-fold domain. This heterodimer forms a
CC histone-like octamer with the TAF6/TAFII70-TAF9/TAFII31 heterodimer.
CC Interacts with P65/RELA homodimers and P65/RELA-REL heterodimers.
CC Interaction with POU2AF1, via its C-terminal activation domain, is
CC required for octamer-dependent transcription (By similarity). Interacts
CC with ZNF628 (PubMed:31932482). {ECO:0000250|UniProtKB:Q92750,
CC ECO:0000269|PubMed:31932482}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q92750}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q92750}. Note=Export into the cytoplasm is
CC mediated by a CRM1-independent nuclear export pathway and not by
CC phosphorylation. {ECO:0000250|UniProtKB:Q92750}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the testes and ovary, whereas
CC lower levels are detected in most other tissues.
CC {ECO:0000269|PubMed:11557891}.
CC -!- DISRUPTION PHENOTYPE: Deficient female mice are infertile due to a
CC granulosa cell defect preventing normal follicle formation. Deficient
CC male are fertile when young, but subsequently exhibit an age-dependent
CC progressive loss of germ cells and misregulation of several genes
CC required for normal spermatogenesis. {ECO:0000269|PubMed:11557891,
CC ECO:0000269|PubMed:15774719}.
CC -!- SIMILARITY: Belongs to the TAF4 family. {ECO:0000305}.
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DR EMBL; AC102692; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC125091; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC133172; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466622; EDL01590.1; -; Genomic_DNA.
DR EMBL; BQ952832; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS89195.1; -.
DR RefSeq; NP_001093919.1; NM_001100449.1.
DR AlphaFoldDB; G5E8Z2; -.
DR SMR; G5E8Z2; -.
DR ComplexPortal; CPX-959; General transcription factor complex TFIID, Taf4b variant.
DR IntAct; G5E8Z2; 1.
DR STRING; 10090.ENSMUSP00000126909; -.
DR iPTMnet; G5E8Z2; -.
DR PhosphoSitePlus; G5E8Z2; -.
DR EPD; G5E8Z2; -.
DR PaxDb; G5E8Z2; -.
DR PeptideAtlas; G5E8Z2; -.
DR PRIDE; G5E8Z2; -.
DR ProteomicsDB; 259352; -.
DR GeneID; 72504; -.
DR KEGG; mmu:72504; -.
DR UCSC; uc008edl.2; mouse.
DR CTD; 6875; -.
DR MGI; MGI:2152345; Taf4b.
DR eggNOG; KOG2341; Eukaryota.
DR HOGENOM; CLU_010576_2_0_1; -.
DR InParanoid; G5E8Z2; -.
DR OrthoDB; 735381at2759; -.
DR PhylomeDB; G5E8Z2; -.
DR TreeFam; TF316520; -.
DR Reactome; R-MMU-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-MMU-6804756; Regulation of TP53 Activity through Phosphorylation.
DR Reactome; R-MMU-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-MMU-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-MMU-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-MMU-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR BioGRID-ORCS; 72504; 1 hit in 71 CRISPR screens.
DR PRO; PR:G5E8Z2; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; G5E8Z2; protein.
DR Genevisible; G5E8Z2; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0001650; C:fibrillar center; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005669; C:transcription factor TFIID complex; ISO:MGI.
DR GO; GO:0005667; C:transcription regulator complex; TAS:MGI.
DR GO; GO:0003677; F:DNA binding; ISO:MGI.
DR GO; GO:0051059; F:NF-kappaB binding; ISO:MGI.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0042789; P:mRNA transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR GO; GO:2000648; P:positive regulation of stem cell proliferation; IMP:CACAO.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; ISO:MGI.
DR GO; GO:0006468; P:protein phosphorylation; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; TAS:MGI.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR GO; GO:0006366; P:transcription by RNA polymerase II; TAS:MGI.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd08045; TAF4; 1.
DR Gene3D; 1.10.20.10; -; 1.
DR Gene3D; 1.20.120.1110; -; 1.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR045144; TAF4.
DR InterPro; IPR007900; TAF4_C.
DR InterPro; IPR037249; TAFH/NHR1_dom_sf.
DR InterPro; IPR003894; TAFH_NHR1.
DR PANTHER; PTHR15138; PTHR15138; 1.
DR Pfam; PF05236; TAF4; 1.
DR Pfam; PF07531; TAFH; 1.
DR SMART; SM00549; TAFH; 1.
DR SUPFAM; SSF158553; SSF158553; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR PROSITE; PS51119; TAFH; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Differentiation; Nucleus; Oogenesis;
KW Phosphoprotein; Reference proteome; Spermatogenesis; Transcription;
KW Transcription regulation.
FT CHAIN 1..850
FT /note="Transcription initiation factor TFIID subunit 4B"
FT /id="PRO_0000430940"
FT DOMAIN 256..353
FT /note="TAFH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00440"
FT DOMAIN 642..691
FT /note="Histone-fold"
FT /evidence="ECO:0000250|UniProtKB:Q92750"
FT REGION 99..240
FT /note="Sufficient for interaction with ZNF628"
FT /evidence="ECO:0000269|PubMed:31932482"
FT REGION 504..526
FT /note="Required for interaction with P65/RELA"
FT /evidence="ECO:0000250|UniProtKB:Q92750"
FT REGION 788..812
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 818..850
FT /note="Required for interaction with TAF12"
FT /evidence="ECO:0000250|UniProtKB:Q92750"
FT MOTIF 509..549
FT /note="Nuclear export signal"
FT /evidence="ECO:0000250|UniProtKB:Q92750"
FT COMPBIAS 796..812
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 584
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q92750"
FT CONFLICT 799
FT /note="K -> Q (in Ref. 3; BQ952832)"
FT /evidence="ECO:0000305"
FT CONFLICT 808
FT /note="S -> F (in Ref. 3; BQ952832)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 850 AA; 89528 MW; D943417A8B31D724 CRC64;
MPAGLTEPAG AAAPVVSSAS GAVTMAPVAA LPVRVEGTPV ALGPVTKAPV SVCVESVAPQ
PLPAPVGTLV TKVVPVTALP KLGSPRLPAP QIVTVKTPGT TTIQLPANLQ LPPGTVLIKS
NSGQLMLVSP QQAVTGAKTT SNITPRPAVP ANTQTVKICT VPNSSSQLMK KVTVAPVKNL
TQIGTTVATT ASSTSSGQPV ALPSSVITVT PAKLVNTVST LKSSSLGVLS TPSNDARLKA
ETSVAAQTAL PPTVLENVKK CKNFLSMLIK LACSGSQSPE MGQNVKRLVE QLLDAEIEAE
EFTRKLYIEL KSAPQPHLVP FLKKSVVALR QLLPNSQSFI ENCVKEVSGD VVISSCTMTT
ATSPVVTSTV SPVLVSGATA PRTLSVQQTL NPLAGPGVAN TGVVTLHSVA PAAATGGTTA
ATVLLQTSKP LTTSVPNTVA AVSLQPENPV VSGAAVTLAI PSATFGEASA TPLCLPSAKP
AITSAGTKAD KPAIGTPVQI VTQPSTLLPQ AAGIPQTAKV KQLVVQQPSG SSVNHVTSIS
HSSPLSTQNC GQKTPVNAVM PTSSIIKQIT LPGNKLLSLQ AQRSSIQSNK IKENGPTCFR
GEDDINDVTF MAEVNLDEEN ACILAAHSDF VGTLIQSCKE EPFLVIGALQ KRILDIGKKH
DITELNSDAV NLISHATQER LRGLLEKLTT IAQHRMTIYK GSENYILSTD TRSQLKFLEK
LDQLEKQRKD LEEREMLLKA AKSRSNKEDP EQLRLKQKAK ELQQLELAQI QYRDANLTAL
AAIGPRKKRP LESGNESFKD NPSTSGTSSL TATKPFRPRI TRICLRDLIF CMEQEREMKY
SRALYLALLK
