TAF5_CAEEL
ID TAF5_CAEEL Reviewed; 648 AA.
AC G5EF68;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Transcription initiation factor TFIID subunit 5 {ECO:0000305};
DE AltName: Full=TBP-associated transcription factor family member taf-5 {ECO:0000312|WormBase:F30F8.8};
GN Name=taf-5 {ECO:0000312|WormBase:F30F8.8};
GN ORFNames=F30F8.8 {ECO:0000312|WormBase:F30F8.8};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP NOMENCLATURE.
RX PubMed=11963920; DOI=10.1101/gad.976402;
RA Tora L.;
RT "A unified nomenclature for TATA box binding protein (TBP)-associated
RT factors (TAFs) involved in RNA polymerase II transcription.";
RL Genes Dev. 16:673-675(2002).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=12458202; DOI=10.1074/jbc.m211056200;
RA Walker A.K., Blackwell T.K.;
RT "A broad but restricted requirement for TAF-5 (human TAFII100) for
RT embryonic transcription in Caenorhabditis elegans.";
RL J. Biol. Chem. 278:6181-6186(2003).
CC -!- FUNCTION: The TFIID basal transcription factor complex plays a major
CC role in the initiation of RNA polymerase II (Pol II)-dependent
CC transcription (By similarity). TFIID recognizes and binds promoters via
CC its subunit tbp-1, a TATA-box-binding protein, and promotes assembly of
CC the pre-initiation complex (PIC) (By similarity). The TFIID complex
CC consists of tbp-1 and TBP-associated factors (TAFs), including taf-5
CC (By similarity). Essential for early embryonic development, but not
CC required for transcription of some genes; probably acts via activating
CC transcription initiation by RNA Pol II, as part of the TFIID complex
CC (PubMed:12458202). {ECO:0000250|UniProtKB:Q15542,
CC ECO:0000269|PubMed:12458202}.
CC -!- SUBUNIT: Component of the TFIID basal transcription factor complex,
CC composed of TATA-box-binding protein tbp-1, and a number of TBP-
CC associated factors (TAFs). {ECO:0000250|UniProtKB:Q15542}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12458202}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos (at protein level).
CC {ECO:0000269|PubMed:12458202}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes arrested
CC development at 90-100 cells and inhibits differentiation
CC (PubMed:12458202). The two E cell daughters (E2 cells), which form the
CC endoderm, divide abnormally early (PubMed:12458202). Phosphorylation of
CC the RNA Pol II large subunit C-terminal domain (CTD) is reduced
CC significantly in embryos (PubMed:12458202). Reduces expression of a
CC range of genes, including let-858, rps-5, hsp16.2, and pes-10, but has
CC little or no effect on expression of cki-2 and sur-5 (PubMed:12458202).
CC Has little or no effect on levels of the TFIID subunits taf-10 and taf-
CC 4 (PubMed:12458202). {ECO:0000269|PubMed:12458202}.
CC -!- SIMILARITY: Belongs to the WD repeat TAF5 family. {ECO:0000305}.
CC -!- CAUTION: The gene name for this protein was previously taf-4
CC (PubMed:11963920). The nomenclature was changed in PMID:11963920 and so
CC readers should take care to avoid confusion (PubMed:11963920).
CC {ECO:0000303|PubMed:11963920}.
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DR EMBL; BX284601; CAB03035.2; -; Genomic_DNA.
DR PIR; T21580; T21580.
DR RefSeq; NP_492169.2; NM_059768.5.
DR AlphaFoldDB; G5EF68; -.
DR SMR; G5EF68; -.
DR IntAct; G5EF68; 1.
DR STRING; 6239.F30F8.8.2; -.
DR EPD; G5EF68; -.
DR PaxDb; G5EF68; -.
DR PeptideAtlas; G5EF68; -.
DR EnsemblMetazoa; F30F8.8.1; F30F8.8.1; WBGene00006386.
DR EnsemblMetazoa; F30F8.8.2; F30F8.8.2; WBGene00006386.
DR GeneID; 172552; -.
DR KEGG; cel:CELE_F30F8.8; -.
DR CTD; 172552; -.
DR WormBase; F30F8.8; CE39361; WBGene00006386; taf-5.
DR eggNOG; KOG0263; Eukaryota.
DR GeneTree; ENSGT00940000153342; -.
DR HOGENOM; CLU_005884_2_1_1; -.
DR InParanoid; G5EF68; -.
DR OMA; RCAFAPE; -.
DR OrthoDB; 620721at2759; -.
DR PhylomeDB; G5EF68; -.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-CEL-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-CEL-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-CEL-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-CEL-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00006386; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0005669; C:transcription factor TFIID complex; IBA:GO_Central.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd08044; TAF5_NTD2; 1.
DR Gene3D; 1.25.40.500; -; 1.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR037783; Taf5.
DR InterPro; IPR007582; TFIID_NTD2.
DR InterPro; IPR037264; TFIID_NTD2_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19879; PTHR19879; 1.
DR Pfam; PF04494; TFIID_NTD2; 1.
DR Pfam; PF00400; WD40; 3.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00667; LisH; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF160897; SSF160897; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; WD repeat.
FT CHAIN 1..648
FT /note="Transcription initiation factor TFIID subunit 5"
FT /id="PRO_0000455414"
FT DOMAIN 35..67
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT REPEAT 317..358
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 392..431
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 433..472
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 475..514
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 517..556
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 560..599
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 648 AA; 72559 MW; B04FAF0F0639E5DE CRC64;
MDSENSSSHS ISSPQMFQNT HNNSAMEDNL LSRPMNNESL QMIIGYLRRN GLTETEELLT
REAGPVLRVE GSNGLPPEEA ISVEFDTFVQ HANDCTDVVQ AEFSQLLFPI FAHSYIALIE
KHAATARIFF NRFKIFIPEC FSEFVYQLSL IEDAMTLRAN EHVHILRENK FLVRLSRPTL
KHLESIQTRV IGVKNIIAKH ICIENADEVS TNRTTIETQM GGILGVTSKS DKRHKMMFSV
LKDELMQNIE KRKTKGKDWK DMGKKMQTHC PQADRIPLPP ISEHLREERR NWLRDVGKMA
IISAESPVSI CMYTTVNAPI GVASCDFTDD SSLIAMGLSD SSIVMNAMDP MNKMKKLRDM
EFLDKIDIET ADNVQSQMFD LQGSTTSVRY TGHGGPVFSV NFSPDRRLLI SSAGDRTVRL
WSMETQRNAV IYRTPAVVWQ AQFCSRGYYF ATASADKTAA MWSTDRMHPL RIFADPYGDV
GCIDYHPNCN YIAGGSDDRY VRVWDVCSGT RVRIFSGHKA SIIAVKFSPC GRYIVSLDAI
GNLMIWDLAY QRLVAAEITE QAGTKGSITF SRDGGVFAVS HGNSSIQLYS LDTLIGTVLA
AGQNDSYIEP KVNLDGFNIG SYATKETAVI GLHFTRRNLL LGFGCFGQ
