TAF5_DROME
ID TAF5_DROME Reviewed; 704 AA.
AC P49846; Q9V5R1;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=Transcription initiation factor TFIID subunit 5;
DE AltName: Full=TAFII-80;
DE AltName: Full=Transcription initiation factor TFIID 85 kDa subunit;
DE Short=p85;
GN Name=Taf5; Synonyms=TAF80; ORFNames=CG7704;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 105-120; 272-280 AND
RP 365-374, FUNCTION, AND INTERACTION WITH TBP AND TAF4.
RC TISSUE=Embryo;
RX PubMed=8247000; DOI=10.1128/mcb.13.12.7859-7863.1993;
RA Kokubo T., Gong D.-W., Yamashita S., Takada R., Roeder R.G., Horikoshi M.,
RA Nakatani Y.;
RT "Molecular cloning, expression, and characterization of the Drosophila 85-
RT kilodalton TFIID subunit.";
RL Mol. Cell. Biol. 13:7859-7863(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE, AND PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Embryo;
RX PubMed=8483503; DOI=10.1038/363176a0;
RA Dynlacht B.D., Weinzierl R.O.J., Admon A., Tjian R.;
RT "The dTAFII80 subunit of Drosophila TFIID contains beta-transducin
RT repeats.";
RL Nature 363:176-179(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: TFIID is a multimeric protein complex that plays a central
CC role in mediating promoter responses to various activators and
CC repressors. May play a role in helping to anchor Taf4 within the TFIID
CC complex. May be involved in transducing signals from various
CC transcriptional regulators to the RNA polymerase II transcription
CC machinery. {ECO:0000269|PubMed:8247000}.
CC -!- SUBUNIT: Belongs to the TFIID complex which is composed of TATA binding
CC protein (Tbp) and a number of TBP-associated factors (Tafs). Interacts
CC with Tbp and Taf4. {ECO:0000269|PubMed:8247000}.
CC -!- INTERACTION:
CC P49846; Q9VWF2: e(y)3; NbExp=5; IntAct=EBI-15595394, EBI-131186;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the WD repeat TAF5 family. {ECO:0000305}.
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DR EMBL; U06460; AAC46481.1; -; mRNA.
DR EMBL; AE013599; AAF58737.1; -; Genomic_DNA.
DR EMBL; AY051960; AAK93384.1; -; mRNA.
DR PIR; S33263; S33263.
DR RefSeq; NP_476957.1; NM_057609.6.
DR AlphaFoldDB; P49846; -.
DR SMR; P49846; -.
DR BioGRID; 71045; 25.
DR DIP; DIP-48906N; -.
DR IntAct; P49846; 8.
DR STRING; 7227.FBpp0087335; -.
DR PaxDb; P49846; -.
DR PRIDE; P49846; -.
DR DNASU; 47900; -.
DR EnsemblMetazoa; FBtr0088240; FBpp0087335; FBgn0010356.
DR GeneID; 47900; -.
DR KEGG; dme:Dmel_CG7704; -.
DR CTD; 6877; -.
DR FlyBase; FBgn0010356; Taf5.
DR VEuPathDB; VectorBase:FBgn0010356; -.
DR eggNOG; KOG0263; Eukaryota.
DR GeneTree; ENSGT00940000153342; -.
DR HOGENOM; CLU_005884_2_1_1; -.
DR InParanoid; P49846; -.
DR OMA; RCAFAPE; -.
DR OrthoDB; 620721at2759; -.
DR PhylomeDB; P49846; -.
DR Reactome; R-DME-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DME-6804756; Regulation of TP53 Activity through Phosphorylation.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-DME-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DME-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DME-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DME-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR BioGRID-ORCS; 47900; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 47900; -.
DR PRO; PR:P49846; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0010356; Expressed in secondary oocyte and 22 other tissues.
DR ExpressionAtlas; P49846; baseline and differential.
DR Genevisible; P49846; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005669; C:transcription factor TFIID complex; IDA:FlyBase.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:FlyBase.
DR CDD; cd08044; TAF5_NTD2; 1.
DR Gene3D; 1.25.40.500; -; 1.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR037783; Taf5.
DR InterPro; IPR007582; TFIID_NTD2.
DR InterPro; IPR037264; TFIID_NTD2_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19879; PTHR19879; 1.
DR Pfam; PF04494; TFIID_NTD2; 1.
DR Pfam; PF00400; WD40; 6.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF160897; SSF160897; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..704
FT /note="Transcription initiation factor TFIID subunit 5"
FT /id="PRO_0000051256"
FT REPEAT 376..405
FT /note="WD 1"
FT REPEAT 448..478
FT /note="WD 2"
FT REPEAT 490..520
FT /note="WD 3"
FT REPEAT 532..562
FT /note="WD 4"
FT REPEAT 574..604
FT /note="WD 5"
FT REPEAT 616..646
FT /note="WD 6"
FT REGION 287..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 410..411
FT /note="KL -> NV (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 704 AA; 79325 MW; F4E2F12534501EF6 CRC64;
MSLEVSNING GNGTQLSHDK RELLCLLKLI KKYQLKSTEE LLCQEANVSS VELSEISESD
VQQVLGAVLG AGDANRERKH VQSPAQGHKQ SAVTEANAAE ELAKFIDDDS FDAQHYEQAY
KELRTFVEDS LDIYKHELSM VLYPILVQIY FKILASGLRE KAKEFIEKYK CDLDGYYIEG
LFNLLLLSKP EELLENDLVV AMEQDKFVIR MSRDSHSLFK RHIQDRRQEV VADIVSKYLH
FDTYEGMARN KLQCVATAGS HLGEAKRQDN KMRVYYGLLK EVDFQTLTTP APAPEEEDDD
PDAPDRPKKK KPKKDPLLSK KSKSDPNAPS IDRIPLPELK DSDKLLKLKA LREASKRLAL
SKDQLPSAVF YTVLNSHQGV TCAEISDDST MLACGFGDSS VRIWSLTPAK LRTLKDADSL
RELDKESADI NVRMLDDRSG EVTRSLMGHT GPVYRCAFAP EMNLLLSCSE DSTIRLWSLL
TWSCVVTYRG HVYPVWDVRF APHGYYFVSC SYDKTARLWA TDSNQALRVF VGHLSDVDCV
QFHPNSNYVA TGSSDRTVRL WDNMTGQSVR LMTGHKGSVS SLAFSACGRY LASGSVDHNI
IIWDLSNGSL VTTLLRHTST VTTITFSRDG TVLAAAGLDN NLTLWDFHKV TEDYISNHIT
VSHHQDENDE DVYLMRTFPS KNSPFVSLHF TRRNLLMCVG LFKS
