TAF6_ARATH
ID TAF6_ARATH Reviewed; 549 AA.
AC Q9MAU3; B9DGR1; B9DH88;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Transcription initiation factor TFIID subunit 6;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2781;
DE AltName: Full=TATA box associated factor II 59;
DE AltName: Full=TBP-associated factor 6;
DE Short=AtTAF6;
DE AltName: Full=Transcription initiation factor TFIID subunit D5;
GN Name=TAF6; Synonyms=EMB2781, TAFII59; OrderedLocusNames=At1g04950;
GN ORFNames=F13M7.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, NOMENCLATURE, AND TISSUE
RP SPECIFICITY.
RX PubMed=15527982; DOI=10.1016/j.gene.2004.08.023;
RA Lago C., Clerici E., Mizzi L., Colombo L., Kater M.M.;
RT "TBP-associated factors in Arabidopsis.";
RL Gene 342:231-241(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=17340043; DOI=10.1007/s11103-007-9135-1;
RA Lawit S.J., O'Grady K., Gurley W.B., Czarnecka-Verner E.;
RT "Yeast two-hybrid map of Arabidopsis TFIID.";
RL Plant Mol. Biol. 64:73-87(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Flower, and Silique;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=16039640; DOI=10.1016/j.ydbio.2005.06.006;
RA Lago C., Clerici E., Dreni L., Horlow C., Caporali E., Colombo L.,
RA Kater M.M.;
RT "The Arabidopsis TFIID factor AtTAF6 controls pollen tube growth.";
RL Dev. Biol. 285:91-100(2005).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-434, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: TAFs are components of the transcription factor IID (TFIID)
CC complex that is essential for mediating regulation of RNA polymerase
CC transcription. Required for proper pollen function. May stabilize the
CC interaction of TFIID with selected promoters. Not redundant with TAF6B.
CC {ECO:0000269|PubMed:16039640}.
CC -!- SUBUNIT: Component of the TFIID complex. TFIID is composed of TATA
CC binding protein (TBP) and a number of TBP-associated factors (TAFs)
CC whose MWs range from 14-217 kDa.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, inflorescences and
CC siliques. {ECO:0000269|PubMed:15527982, ECO:0000269|PubMed:16039640}.
CC -!- DEVELOPMENTAL STAGE: Expressed in germinating pollen.
CC {ECO:0000269|PubMed:16039640}.
CC -!- DISRUPTION PHENOTYPE: Lethal when homozygote. Reduced pollen tube
CC growth when heterozygote. {ECO:0000269|PubMed:16039640}.
CC -!- SIMILARITY: Belongs to the TAF6 family. {ECO:0000305}.
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DR EMBL; AY463621; AAR28023.1; -; mRNA.
DR EMBL; AC004809; AAF40443.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27766.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27767.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27768.1; -; Genomic_DNA.
DR EMBL; AY050962; AAK93639.1; -; mRNA.
DR EMBL; AY091292; AAM14231.1; -; mRNA.
DR EMBL; AK317247; BAH19928.1; -; mRNA.
DR EMBL; AK317438; BAH20105.1; -; mRNA.
DR PIR; A86183; A86183.
DR RefSeq; NP_001030969.1; NM_001035892.3.
DR RefSeq; NP_171987.1; NM_100373.4.
DR RefSeq; NP_849592.1; NM_179261.4.
DR AlphaFoldDB; Q9MAU3; -.
DR SMR; Q9MAU3; -.
DR BioGRID; 24605; 38.
DR IntAct; Q9MAU3; 2.
DR STRING; 3702.AT1G04950.3; -.
DR iPTMnet; Q9MAU3; -.
DR PaxDb; Q9MAU3; -.
DR PRIDE; Q9MAU3; -.
DR ProteomicsDB; 234123; -.
DR EnsemblPlants; AT1G04950.1; AT1G04950.1; AT1G04950.
DR EnsemblPlants; AT1G04950.2; AT1G04950.2; AT1G04950.
DR EnsemblPlants; AT1G04950.3; AT1G04950.3; AT1G04950.
DR GeneID; 839370; -.
DR Gramene; AT1G04950.1; AT1G04950.1; AT1G04950.
DR Gramene; AT1G04950.2; AT1G04950.2; AT1G04950.
DR Gramene; AT1G04950.3; AT1G04950.3; AT1G04950.
DR KEGG; ath:AT1G04950; -.
DR Araport; AT1G04950; -.
DR TAIR; locus:2010667; AT1G04950.
DR eggNOG; KOG2549; Eukaryota.
DR HOGENOM; CLU_021711_1_0_1; -.
DR InParanoid; Q9MAU3; -.
DR OMA; DTSIVCH; -.
DR OrthoDB; 384329at2759; -.
DR PhylomeDB; Q9MAU3; -.
DR PRO; PR:Q9MAU3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9MAU3; baseline and differential.
DR Genevisible; Q9MAU3; AT.
DR GO; GO:0000124; C:SAGA complex; IEA:InterPro.
DR GO; GO:0046695; C:SLIK (SAGA-like) complex; IEA:InterPro.
DR GO; GO:0005669; C:transcription factor TFIID complex; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IEA:InterPro.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IBA:GO_Central.
DR CDD; cd08050; TAF6C; 1.
DR Gene3D; 1.10.20.10; -; 1.
DR Gene3D; 1.25.40.770; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR037796; TAF6.
DR InterPro; IPR011442; TAF6_C.
DR InterPro; IPR046344; TAF6_C_sf.
DR InterPro; IPR004823; TAF_TATA-bd_Histone-like_dom.
DR PANTHER; PTHR10221; PTHR10221; 1.
DR Pfam; PF02969; TAF; 1.
DR Pfam; PF07571; TAF6_C; 1.
DR SMART; SM00803; TAF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..549
FT /note="Transcription initiation factor TFIID subunit 6"
FT /id="PRO_0000424042"
FT DOMAIN 5..78
FT /note="Histone-fold"
FT REGION 399..501
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 412..428
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 447..487
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 434
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT CONFLICT 240
FT /note="R -> V (in Ref. 6; BAH20105)"
FT /evidence="ECO:0000305"
FT CONFLICT 383
FT /note="A -> V (in Ref. 6; BAH19928)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 549 AA; 61365 MW; 9F92A0A019E14B06 CRC64;
MSIVPKETVE VIAQSIGITN LLPEAALMLA PDVEYRVREI MQEAIKCMRH SKRTTLTASD
VDGALNLRNV EPIYGFASGG PFRFRKAIGH RDLFYTDDRE VDFKDVIEAP LPKAPLDTEI
VCHWLAIEGV QPAIPENAPL EVIRAPAETK IHEQKDGPLI DVRLPVKHVL SRELQLYFQK
IAELAMSKSN PPLYKEALVS LASDSGLHPL VPYFTNFIAD EVSNGLNDFR LLFNLMHIVR
SLLQNPHIHI EPYLHQLMPS VVTCLVSRKL GNRFADNHWE LRDFAANLVS LICKRYGTVY
ITLQSRLTRT LVNALLDPKK ALTQHYGAIQ GLAALGHTVV RLLILSNLEP YLSLLEPELN
AEKQKNQMKI YEAWRVYGAL LRAAGLCIHG RLKIFPPLPS PSPSFLHKGK GKGKIISTDP
HKRKLSVDSS ENQSPQKRLI TMDGPDGVHS QDQSGSAPMQ VDNPVENDNP PQNSVQPSSS
EQASDANESE SRNGKVKESG RSRAITMKAI LDQIWKDDLD SGRLLVKLHE LYGDRILPFI
PSTEMSVFL
