TAF8_DANRE
ID TAF8_DANRE Reviewed; 308 AA.
AC Q6P0T2; Q7ZWG5;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Transcription initiation factor TFIID subunit 8;
DE AltName: Full=Protein taube nuss-like;
DE AltName: Full=TBP-associated factor 8;
GN Name=taf8; Synonyms=tbnl;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=SJD; TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18307296; DOI=10.1021/pr700667w;
RA Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J.,
RA Slijper M., Heck A.J.R.;
RT "Online automated in vivo zebrafish phosphoproteomics: from large-scale
RT analysis down to a single embryo.";
RL J. Proteome Res. 7:1555-1564(2008).
CC -!- FUNCTION: The TFIID basal transcription factor complex plays a major
CC role in the initiation of RNA polymerase II (Pol II)-dependent
CC transcription. TFIID recognizes and binds promoters with or without a
CC TATA box via its subunit tbp, a TATA-box-binding protein, and promotes
CC assembly of the pre-initiation complex (PIC). The TFIID complex
CC consists of tbp and TBP-associated factors (TAFs). Mediates both basal
CC and activator-dependent transcription. {ECO:0000250|UniProtKB:Q7Z7C8}.
CC -!- SUBUNIT: Component of the TFIID basal transcription factor complex,
CC composed of TATA-box-binding protein tbp, and a number of TBP-
CC associated factors (TAFs). {ECO:0000250|UniProtKB:Q7Z7C8}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6P0T2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P0T2-2; Sequence=VSP_030556;
CC -!- SIMILARITY: Belongs to the TAF8 family. {ECO:0000305}.
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DR EMBL; BC049422; AAH49422.1; -; mRNA.
DR EMBL; BC065457; AAH65457.1; -; mRNA.
DR RefSeq; NP_956580.1; NM_200286.1. [Q6P0T2-2]
DR RefSeq; XP_005162360.1; XM_005162303.3. [Q6P0T2-1]
DR AlphaFoldDB; Q6P0T2; -.
DR SMR; Q6P0T2; -.
DR STRING; 7955.ENSDARP00000032165; -.
DR iPTMnet; Q6P0T2; -.
DR PaxDb; Q6P0T2; -.
DR Ensembl; ENSDART00000039498; ENSDARP00000032165; ENSDARG00000026806. [Q6P0T2-1]
DR Ensembl; ENSDART00000157514; ENSDARP00000131962; ENSDARG00000026806. [Q6P0T2-1]
DR Ensembl; ENSDART00000164220; ENSDARP00000133427; ENSDARG00000026806. [Q6P0T2-1]
DR Ensembl; ENSDART00000171715; ENSDARP00000130755; ENSDARG00000026806. [Q6P0T2-2]
DR Ensembl; ENSDART00000179137; ENSDARP00000144389; ENSDARG00000114197. [Q6P0T2-1]
DR GeneID; 393256; -.
DR KEGG; dre:393256; -.
DR CTD; 129685; -.
DR ZFIN; ZDB-GENE-040426-1013; taf8.
DR eggNOG; KOG4336; Eukaryota.
DR GeneTree; ENSGT00390000017567; -.
DR HOGENOM; CLU_070829_0_0_1; -.
DR InParanoid; Q6P0T2; -.
DR OrthoDB; 1290064at2759; -.
DR PhylomeDB; Q6P0T2; -.
DR TreeFam; TF316311; -.
DR Reactome; R-DRE-6807505; RNA polymerase II transcribes snRNA genes.
DR PRO; PR:Q6P0T2; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 23.
DR Bgee; ENSDARG00000026806; Expressed in testis and 22 other tissues.
DR ExpressionAtlas; Q6P0T2; baseline.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005669; C:transcription factor TFIID complex; ISS:UniProtKB.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd08049; TAF8; 1.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR006565; BTP.
DR InterPro; IPR009072; Histone-fold.
DR InterPro; IPR037818; TAF8.
DR InterPro; IPR019473; TFIID_su8_C.
DR PANTHER; PTHR46469; PTHR46469; 1.
DR Pfam; PF07524; Bromo_TP; 1.
DR Pfam; PF10406; TAF8_C; 1.
DR SMART; SM00576; BTP; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..308
FT /note="Transcription initiation factor TFIID subunit 8"
FT /id="PRO_0000315400"
FT DOMAIN 37..104
FT /note="Histone-fold"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 239..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 295..308
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 7..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18307296"
FT VAR_SEQ 210..249
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_030556"
SQ SEQUENCE 308 AA; 33912 MW; 8A3035BC898AE151 CRC64;
MADSVMMGSG SLNSGSRSGS SKTCTSPTEN YQLARRRTLQ VVVSALLTEC GFDSAEKAVV
ETLTEMMQSY ITEVGRCAKA NCEHTARSTP TLSDVVITLV EMGFNVDTLP VYAKRSQRMV
ITAPPITNAP VVPKSLTAGQ KRTHPAHIPS HFPDFPDPHT YIRTPTFREP VSDYQVVREK
AASQRRDVER ALTRFMAKTG ETQSLFKDDI SAFPLIAAKP SPIPYLSALL PSELELQSLE
ETDSSEQDDQ MDNDNNTSHI MQDDSGADKE NEVLPPGGVV PSGKANEENM IDNPYLRPVK
KPKVRRKK
