ID   TAG2U_AGEOR             Reviewed;          68 AA.
AC   Q5Y4W6;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=U2-agatoxin-Ao1u;
DE            Short=U2-AGTX-Ao1u;
DE   AltName: Full=Agel_20;
DE   Flags: Precursor;
OS   Agelena orientalis (Funnel-web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Agelenidae; Agelena.
OX   NCBI_TaxID=293813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=15688451; DOI=10.1002/prot.20390;
RA   Kozlov S.A., Malyavka A., McCutchen B., Lu A., Schepers E., Herrmann R.,
RA   Grishin E.V.;
RT   "A novel strategy for the identification of toxinlike structures in spider
RT   venom.";
RL   Proteins 59:131-140(2005).
CC   -!- FUNCTION: Insect active toxin causing rapid but reversible paralysis in
CC       crickets. No activity shown in mammals. Does not show effect on
CC       mammalian voltage-gated calcium channels (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 01 (U2-agtx) family.
CC       {ECO:0000305}.
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DR   EMBL; AY681317; AAU93674.1; -; mRNA.
DR   AlphaFoldDB; Q5Y4W6; -.
DR   SMR; Q5Y4W6; -.
DR   ArachnoServer; AS000094; U2-agatoxin-Ao1u.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Knottin; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..34
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5000093639"
FT   CHAIN           35..68
FT                   /note="U2-agatoxin-Ao1u"
FT                   /id="PRO_5000093640"
FT   DISULFID        36..52
FT                   /evidence="ECO:0000250"
FT   DISULFID        43..57
FT                   /evidence="ECO:0000250"
FT   DISULFID        51..67
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   68 AA;  7458 MW;  48D9C006F80514DC CRC64;
     MKAIISLLLI SAMVFSMIEA VPLEEGLQLF EGERGCLPHN RFCNALSGPR CCSGLTCKEL
     NIWASKCL