TAGAP_HUMAN
ID TAGAP_HUMAN Reviewed; 731 AA.
AC Q8N103; Q2NKM8; Q8NI40; Q96KZ2; Q96QA2;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=T-cell activation Rho GTPase-activating protein;
DE AltName: Full=T-cell activation GTPase-activating protein;
GN Name=TAGAP; Synonyms=TAGAP1; ORFNames=FKSG15;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND FUNCTION.
RX PubMed=15177553; DOI=10.1016/j.ygeno.2003.12.019;
RA Mao M., Biery M.C., Kobayashi S.V., Ward T., Schimmack G., Burchard J.,
RA Schelter J.M., Dai H., He Y.D., Linsley P.S.;
RT "T lymphocyte activation gene identification by coregulated expression on
RT DNA microarrays.";
RL Genomics 83:989-999(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RA Wang Y.-G., Gong L.;
RT "FKSG15, a novel 57 kDa protein, shares the N-terminal region with FOP.";
RL Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Spleen;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-400, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: May function as a GTPase-activating protein and may play
CC important roles during T-cell activation.
CC {ECO:0000269|PubMed:15177553}.
CC -!- INTERACTION:
CC Q8N103; O75031: HSF2BP; NbExp=3; IntAct=EBI-12136823, EBI-7116203;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8N103-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N103-2; Sequence=VSP_015869;
CC Name=3; Synonyms=FKSG15;
CC IsoId=Q8N103-3; Sequence=VSP_015868, VSP_015870;
CC Name=4;
CC IsoId=Q8N103-4; Sequence=VSP_015871, VSP_015872;
CC -!- MISCELLANEOUS: [Isoform 3]: Dubious isoform. The N-terminus appears to
CC be derived from exons of the CEP43 locus which is located on the
CC opposing strand of chromosome 6 at a distance of several Mb.
CC {ECO:0000305}.
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DR EMBL; AF385429; AAM43830.1; -; mRNA.
DR EMBL; AF385430; AAM43831.1; -; mRNA.
DR EMBL; AF314817; AAL16675.1; -; mRNA.
DR EMBL; AK097090; BAC04947.1; -; mRNA.
DR EMBL; AL035530; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC015859; AAH15859.1; -; mRNA.
DR EMBL; BC111731; AAI11732.1; -; mRNA.
DR CCDS; CCDS5261.1; -. [Q8N103-1]
DR CCDS; CCDS5262.1; -. [Q8N103-2]
DR CCDS; CCDS5263.1; -. [Q8N103-4]
DR RefSeq; NP_001265662.1; NM_001278733.1.
DR RefSeq; NP_473455.2; NM_054114.4. [Q8N103-1]
DR RefSeq; NP_620165.1; NM_138810.3. [Q8N103-4]
DR RefSeq; NP_687034.1; NM_152133.2. [Q8N103-2]
DR AlphaFoldDB; Q8N103; -.
DR SMR; Q8N103; -.
DR BioGRID; 125587; 20.
DR ELM; Q8N103; -.
DR IntAct; Q8N103; 3.
DR STRING; 9606.ENSP00000356033; -.
DR iPTMnet; Q8N103; -.
DR PhosphoSitePlus; Q8N103; -.
DR BioMuta; TAGAP; -.
DR DMDM; 74750874; -.
DR jPOST; Q8N103; -.
DR MassIVE; Q8N103; -.
DR MaxQB; Q8N103; -.
DR PaxDb; Q8N103; -.
DR PeptideAtlas; Q8N103; -.
DR PRIDE; Q8N103; -.
DR ProteomicsDB; 71499; -. [Q8N103-1]
DR ProteomicsDB; 71500; -. [Q8N103-2]
DR ProteomicsDB; 71501; -. [Q8N103-3]
DR ProteomicsDB; 71502; -. [Q8N103-4]
DR Antibodypedia; 33449; 186 antibodies from 22 providers.
DR DNASU; 117289; -.
DR Ensembl; ENST00000326965.7; ENSP00000322650.6; ENSG00000164691.18. [Q8N103-2]
DR Ensembl; ENST00000338313.5; ENSP00000340217.5; ENSG00000164691.18. [Q8N103-4]
DR Ensembl; ENST00000367066.8; ENSP00000356033.4; ENSG00000164691.18. [Q8N103-1]
DR GeneID; 117289; -.
DR KEGG; hsa:117289; -.
DR MANE-Select; ENST00000367066.8; ENSP00000356033.4; NM_054114.5; NP_473455.2.
DR UCSC; uc003qrz.5; human. [Q8N103-1]
DR CTD; 117289; -.
DR DisGeNET; 117289; -.
DR GeneCards; TAGAP; -.
DR HGNC; HGNC:15669; TAGAP.
DR HPA; ENSG00000164691; Tissue enhanced (bone marrow, lymphoid tissue).
DR MalaCards; TAGAP; -.
DR MIM; 609667; gene.
DR neXtProt; NX_Q8N103; -.
DR OpenTargets; ENSG00000164691; -.
DR PharmGKB; PA38016; -.
DR VEuPathDB; HostDB:ENSG00000164691; -.
DR eggNOG; KOG4724; Eukaryota.
DR GeneTree; ENSGT00940000157993; -.
DR HOGENOM; CLU_014209_1_0_1; -.
DR InParanoid; Q8N103; -.
DR OMA; HRDSKEP; -.
DR OrthoDB; 148399at2759; -.
DR PhylomeDB; Q8N103; -.
DR TreeFam; TF331062; -.
DR PathwayCommons; Q8N103; -.
DR Reactome; R-HSA-8980692; RHOA GTPase cycle.
DR Reactome; R-HSA-9013148; CDC42 GTPase cycle.
DR Reactome; R-HSA-9013149; RAC1 GTPase cycle.
DR SignaLink; Q8N103; -.
DR SIGNOR; Q8N103; -.
DR BioGRID-ORCS; 117289; 9 hits in 1074 CRISPR screens.
DR ChiTaRS; TAGAP; human.
DR GenomeRNAi; 117289; -.
DR Pharos; Q8N103; Tbio.
DR PRO; PR:Q8N103; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q8N103; protein.
DR Bgee; ENSG00000164691; Expressed in bone marrow cell and 153 other tissues.
DR ExpressionAtlas; Q8N103; baseline and differential.
DR Genevisible; Q8N103; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Guanine-nucleotide releasing factor; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..731
FT /note="T-cell activation Rho GTPase-activating protein"
FT /id="PRO_0000056719"
FT DOMAIN 88..277
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 288..421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 464..507
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 641..662
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 298..334
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..401
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 464..491
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 492..507
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 400
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT VAR_SEQ 1..209
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_015868"
FT VAR_SEQ 1..178
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15177553"
FT /id="VSP_015869"
FT VAR_SEQ 210..261
FT /note="KHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQSLSFEAQKDLNNK
FT -> MAATAAAVVAEEDTELRDLLVQTLENSGVLNRIKAELRAAVFLALEEQEKVE (in
FT isoform 3)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_015870"
FT VAR_SEQ 263..266
FT /note="KTLV -> CSAY (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_015871"
FT VAR_SEQ 267..731
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_015872"
FT VARIANT 346
FT /note="G -> D (in dbSNP:rs35263580)"
FT /id="VAR_049146"
SQ SEQUENCE 731 AA; 80703 MW; 9D8768258A0A681E CRC64;
MKLRSSHNAS KTLNANNMET LIECQSEGDI KEHPLLASCE SEDSICQLIE VKKRKKVLSW
PFLMRRLSPA SDFSGALETD LKASLFDQPL SIICGDSDTL PRPIQDILTI LCLKGPSTEG
IFRRAANEKA RKELKEELNS GDAVDLERLP VHLLAVVFKD FLRSIPRKLL SSDLFEEWMG
ALEMQDEEDR IEALKQVADK LPRPNLLLLK HLVYVLHLIS KNSEVNRMDS SNLAICIGPN
MLTLENDQSL SFEAQKDLNN KVKTLVEFLI DNCFEIFGEN IPVHSSITSD DSLEHTDSSD
VSTLQNDSAY DSNDPDVESN SSSGISSPSR QPQVPMATAA GLDSAGPQDA REVSPEPIVS
TVARLKSSLA QPDRRYSEPS MPSSQECLES RVTNQTLTKS EGDFPVPRVG SRLESEEAED
PFPEEVFPAV QGKTKRPVDL KIKNLAPGSV LPRALVLKAF SSSSLDASSD SSPVASPSSP
KRNFFSRHQS FTTKTEKGKP SREIKKHSMS FTFAPHKKVL TKNLSAGSGK SQDFTRDHVP
RGVRKESQLA GRIVQENGCE THNQTARGFC LRPHALSVDD VFQGADWERP GSPPSYEEAM
QGPAARLVAS ESQTVGSMTV GSMRARMLEA HCLLPPLPPA HHVEDSRHRG SKEPLPGHGL
SPLPERWKQS RTVHASGDSL GHVSGPGRPE LLPLRTVSES VQRNKRDCLV RRCSQPVFEA
DQFQYAKESY I
