ID   TAGJ_PSEAE              Reviewed;         281 AA.
AC   Q9I746;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Type VI secretion system accessory component TagJ {ECO:0000303|PubMed:22906320};
GN   Name=tagJ {ECO:0000303|PubMed:22906320}; OrderedLocusNames=PA0086;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, INTERACTION WITH TSSB1, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PAK;
RX   PubMed=22906320; DOI=10.1111/j.1365-2958.2012.08204.x;
RA   Lossi N.S., Manoli E., Simpson P., Jones C., Hui K., Dajani R.,
RA   Coulthurst S.J., Freemont P., Filloux A.;
RT   "The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel
RT   subcomplex in the bacterial type VI secretion system.";
RL   Mol. Microbiol. 86:437-456(2012).
RN   [3] {ECO:0007744|PDB:4UQX, ECO:0007744|PDB:4UQY, ECO:0007744|PDB:4UQZ}
RP   X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 21-281, FUNCTION, AND INTERACTION
RP   WITH TSSB1 AND CLPV1.
RX   PubMed=25305017; DOI=10.1074/jbc.m114.600510;
RA   Foerster A., Planamente S., Manoli E., Lossi N.S., Freemont P.S.,
RA   Filloux A.;
RT   "Coevolution of the ATPase ClpV, the sheath proteins TssB and TssC, and the
RT   accessory protein TagJ/HsiE1 distinguishes type VI secretion classes.";
RL   J. Biol. Chem. 289:33032-33043(2014).
CC   -!- FUNCTION: Component of the H1 type VI (H1-T6SS) secretion system that
CC       plays a role in the release of toxins targeting both eukaryotic and
CC       prokaryotic species. Forms a stable complex with TssB1. This complex,
CC       although not crucial for the H1-T6SS function, may fine-tune the
CC       assembly of the system (PubMed:22906320). Plays a role in the
CC       interaction between ClpV1 and the TssC1/TssB1 sheath (PubMed:25305017).
CC       {ECO:0000269|PubMed:22906320, ECO:0000269|PubMed:25305017}.
CC   -!- SUBUNIT: Interacts with TssB1 (via N-terminus) (PubMed:22906320,
CC       PubMed:25305017). Interacts with ClpV1 (PubMed:25305017).
CC       {ECO:0000269|PubMed:22906320, ECO:0000269|PubMed:25305017}.
CC   -!- DISRUPTION PHENOTYPE: Deletion has no detectable effect on the T6SS-
CC       mediated secretion of VgrG1a/c, Hcp1 or Tse3.
CC       {ECO:0000269|PubMed:22906320}.
CC   -!- MISCELLANEOUS: The T6SS protein TagJ/HsiE1 is unique to the H1-T6SS and
CC       absent from the H2- and H3-T6SSs. {ECO:0000305|PubMed:22906320}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE004091; AAG03476.1; -; Genomic_DNA.
DR   PIR; C83635; C83635.
DR   RefSeq; NP_248776.1; NC_002516.2.
DR   RefSeq; WP_003119488.1; NZ_QZGE01000015.1.
DR   PDB; 4UQX; X-ray; 1.20 A; A=21-281.
DR   PDB; 4UQY; X-ray; 1.60 A; A=21-281.
DR   PDB; 4UQZ; X-ray; 1.60 A; A=21-281.
DR   PDBsum; 4UQX; -.
DR   PDBsum; 4UQY; -.
DR   PDBsum; 4UQZ; -.
DR   AlphaFoldDB; Q9I746; -.
DR   SMR; Q9I746; -.
DR   STRING; 287.DR97_3043; -.
DR   PaxDb; Q9I746; -.
DR   PRIDE; Q9I746; -.
DR   DNASU; 879504; -.
DR   EnsemblBacteria; AAG03476; AAG03476; PA0086.
DR   GeneID; 879504; -.
DR   KEGG; pae:PA0086; -.
DR   PATRIC; fig|208964.12.peg.90; -.
DR   PseudoCAP; PA0086; -.
DR   HOGENOM; CLU_087908_1_0_6; -.
DR   OMA; GRYYWIP; -.
DR   PhylomeDB; Q9I746; -.
DR   BioCyc; PAER208964:G1FZ6-88-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR009211; TagJ.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF07024; ImpE; 1.
DR   PIRSF; PIRSF029288; SciE_ImpE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome.
FT   CHAIN           1..281
FT                   /note="Type VI secretion system accessory component TagJ"
FT                   /id="PRO_0000449260"
FT   HELIX           22..28
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           32..45
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           50..62
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           66..79
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           81..83
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           84..104
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           120..131
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           135..148
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          154..156
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          159..162
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          164..168
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   TURN            169..171
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          172..179
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          182..187
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           188..190
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          191..196
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           202..205
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          207..214
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          219..225
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           231..234
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           237..240
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          245..247
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          253..256
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          258..263
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          265..267
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   HELIX           268..270
FT                   /evidence="ECO:0007829|PDB:4UQX"
FT   STRAND          273..276
FT                   /evidence="ECO:0007829|PDB:4UQX"
SQ   SEQUENCE   281 AA;  30362 MW;  C799ECFACA03F4A8 CRC64;
     MADPSFASGR LGSRLQGSIA MIAEELLRAG RLDDALKALQ EQVRSQPSNA TLRIFLFQLL
     AVMGQWARAQ NQLKVVGELD ASALPMVQTY STAIDCEALR REVFAGRLTP VILGQPAEWI
     APLLQALSLD AEGHGEAAQA LREQAFDAAP AVPGRIGEAP FAWLADADTR LGPVLEVIVN
     GRYAWLPMSN LRSLKVEAPS DLRDLVWLPA ELTLANGGAT VALLPARYAE TVEHGDDAAR
     LGRKTEWLDS GLPVGQRLFV TDAGETALFD LRELDFEPTD A