ID   TAGK_AGRFC              Reviewed;         329 AA.
AC   A9CES5;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Tagatose kinase {ECO:0000305};
DE            EC=2.7.1.101 {ECO:0000269|PubMed:26472925};
GN   OrderedLocusNames=Atu3166 {ECO:0000312|EMBL:AAK90220.2};
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY,
RP   INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=26472925; DOI=10.1074/jbc.m115.686857;
RA   Wichelecki D.J., Vetting M.W., Chou L., Al-Obaidi N., Bouvier J.T.,
RA   Almo S.C., Gerlt J.A.;
RT   "ATP-binding cassette (ABC) transport system solute-binding protein-guided
RT   identification of novel D-altritol and galactitol catabolic pathways in
RT   Agrobacterium tumefaciens C58.";
RL   J. Biol. Chem. 290:28963-28976(2015).
CC   -!- FUNCTION: Involved in galactitol and D-altritol catabolism. Catalyzes
CC       the phosphorylation of D-tagatose to D-tagatose 6-phosphate.
CC       {ECO:0000269|PubMed:26472925}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + keto-D-tagatose = ADP + H(+) + keto-D-tagatose 6-
CC         phosphate; Xref=Rhea:RHEA:15513, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:47693, ChEBI:CHEBI:134283,
CC         ChEBI:CHEBI:456216; EC=2.7.1.101;
CC         Evidence={ECO:0000269|PubMed:26472925};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.68 mM for D-tagatose {ECO:0000269|PubMed:26472925};
CC         Note=kcat is 3.0 sec(-1). {ECO:0000269|PubMed:26472925};
CC   -!- PATHWAY: Carbohydrate metabolism. {ECO:0000269|PubMed:26472925}.
CC   -!- INDUCTION: Up-regulated by growth on D-altritol or galactitol.
CC       {ECO:0000269|PubMed:26472925}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the gene does not result in growth
CC       defects for either D-galactitol or D-altritol.
CC       {ECO:0000269|PubMed:26472925}.
CC   -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC       {ECO:0000305}.
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DR   EMBL; AE007870; AAK90220.2; -; Genomic_DNA.
DR   RefSeq; NP_357435.2; NC_003063.2.
DR   AlphaFoldDB; A9CES5; -.
DR   SMR; A9CES5; -.
DR   STRING; 176299.Atu3166; -.
DR   EnsemblBacteria; AAK90220; AAK90220; Atu3166.
DR   KEGG; atu:Atu3166; -.
DR   PATRIC; fig|176299.10.peg.3011; -.
DR   eggNOG; COG0524; Bacteria.
DR   HOGENOM; CLU_027634_6_0_5; -.
DR   OMA; DLWKGRV; -.
DR   PhylomeDB; A9CES5; -.
DR   BioCyc; MetaCyc:MON-20148; -.
DR   Proteomes; UP000000813; Chromosome linear.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0050317; F:tagatose kinase activity; IDA:UniProtKB.
DR   GO; GO:0019402; P:galactitol metabolic process; IDA:UniProtKB.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1190.20; -; 1.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR011611; PfkB_dom.
DR   InterPro; IPR029056; Ribokinase-like.
DR   Pfam; PF00294; PfkB; 1.
DR   SUPFAM; SSF53613; SSF53613; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Carbohydrate metabolism; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..329
FT                   /note="Tagatose kinase"
FT                   /id="PRO_0000446636"
SQ   SEQUENCE   329 AA;  34299 MW;  342B8C3FAFAEC94A CRC64;
     MRQASVLAPH TNGPTVTAGE ILVEIMATTV GDGFLEPQTL IGPFPSGAPA IFIDQVARCG
     GTAGIIAAVG DDDFGRLNIE RLRRDGVDVS AITTIADRPT GSAFVRYREN GARDFVFNIA
     HSAASETRMT DEAQALIEKA GHVHIMGSAF AIAGIGAIIL EAVKSVKARG GSVSFDPNIR
     KELAQGDEGR RLIDDLLAVT DLLLPSGEEL QAASGLDDEE AAIEKLLAAG IGEIVLKRGA
     NGASHFSRAH GRIDAPGLEV EEIDPTGAGD CFGATYLTCR RLGMRPGKAL VYANASGAHN
     VTKRGPMEGA ASLAELDAFM AAQLSEEVL