ID   TALA1_DICDI             Reviewed;        2492 AA.
AC   P0CE95; P54633; Q54FX1;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Talin-A;
DE   AltName: Full=Filopodin;
GN   Name=talA;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=AX2;
RX   PubMed=7698984; DOI=10.1083/jcb.129.1.179;
RA   Kreitmeier M., Gerisch G., Heizer C., Mueller-Taubenberger A.;
RT   "A talin homologue of Dictyostelium rapidly assembles at the leading edge
RT   of cells in response to chemoattractant.";
RL   J. Cell Biol. 129:179-188(1995).
RN   [2]
RP   SEQUENCE REVISION TO 2076; 2080; 2186 AND 2230.
RA   Mueller-Taubenberger A.;
RL   Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=AX2;
RX   PubMed=16782229; DOI=10.1016/j.ejcb.2006.05.008;
RA   Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R.;
RT   "Identification and isolation of Dictyostelium microtubule-associated
RT   protein interactors by tandem affinity purification.";
RL   Eur. J. Cell Biol. 85:1079-1090(2006).
CC   -!- FUNCTION: Actin-binding protein that may be involved in the control of
CC       cell motility and chemotaxis. {ECO:0000269|PubMed:7698984}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000269|PubMed:7698984}. Cytoplasm, cell cortex
CC       {ECO:0000269|PubMed:7698984}. Note=Rapidly assembles at the leading
CC       edge of cells in response to chemoattractant.
CC   -!- CAUTION: This is a full-length TALA gene product. Strain AX2 produces a
CC       full length protein. Strain AX4 has a stop codon in position 1280 which
CC       disrupts the TALA gene and produces a truncated protein (AC P0CE94).
CC       {ECO:0000305}.
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DR   EMBL; U14576; AAC46586.2; -; Genomic_DNA.
DR   PIR; A57036; A57036.
DR   AlphaFoldDB; P0CE95; -.
DR   SMR; P0CE95; -.
DR   STRING; 44689.DDB0233802; -.
DR   ABCD; P0CE95; 2 sequenced antibodies.
DR   eggNOG; KOG4261; Eukaryota.
DR   GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0051015; F:actin filament binding; IEA:InterPro.
DR   GO; GO:0000916; P:actomyosin contractile ring contraction; IMP:CACAO.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   CDD; cd14473; FERM_B-lobe; 1.
DR   Gene3D; 1.20.80.10; -; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR036723; Alpha-catenin/vinculin-like_sf.
DR   InterPro; IPR019749; Band_41_domain.
DR   InterPro; IPR014352; FERM/acyl-CoA-bd_prot_sf.
DR   InterPro; IPR035963; FERM_2.
DR   InterPro; IPR019748; FERM_central.
DR   InterPro; IPR019747; FERM_CS.
DR   InterPro; IPR000299; FERM_domain.
DR   InterPro; IPR032425; FERM_f0.
DR   InterPro; IPR035964; I/LWEQ_dom_sf.
DR   InterPro; IPR002558; ILWEQ_dom.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   Pfam; PF16511; FERM_f0; 1.
DR   Pfam; PF00373; FERM_M; 1.
DR   Pfam; PF01608; I_LWEQ; 1.
DR   SMART; SM00295; B41; 1.
DR   SMART; SM00307; ILWEQ; 1.
DR   SUPFAM; SSF109885; SSF109885; 1.
DR   SUPFAM; SSF47031; SSF47031; 1.
DR   SUPFAM; SSF47220; SSF47220; 4.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS00660; FERM_1; 1.
DR   PROSITE; PS00661; FERM_2; 1.
DR   PROSITE; PS50057; FERM_3; 1.
DR   PROSITE; PS50945; I_LWEQ; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; Cytoplasm; Cytoskeleton.
FT   CHAIN           1..2492
FT                   /note="Talin-A"
FT                   /id="PRO_0000393276"
FT   DOMAIN          84..365
FT                   /note="FERM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00084"
FT   DOMAIN          2250..2492
FT                   /note="I/LWEQ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00292"
SQ   SEQUENCE   2492 AA;  268851 MW;  4B82F6BE6DE7E752 CRC64;
     MSISLKINIV GANTVKTLRF APDMCIQECC THIFEKTNEG GPDHGLYQAH IEGKQSARWL
     AMEKTLQFYD INSDQQLDYK KKHRPQKFKL LDGTIKTQLV DESQNVSEIV NSICKKMGIK
     NPEEYSLMNS AGAWLNNTQI LSEQGISEND ITVLMKKFFF NDANIDRNDP VQLHLLFVQC
     RDGIIEGKYP TQREESLALS ALQCQVQLGD YNPTKHEPGF LTLKDYLPLQ WLKSKGVEKD
     IFKEHKKLVS MTEVNAKYRY VQLCRSLKTY GMTSFDVKIR EYGKKKMVDH ILGITREQML
     LMLTETKEVI MTHPLKHIKR WAATDKSFTL DFGDHETEYL ILQTPNPEQI SQLIGGYIEI
     IMKARKDSSK VIEKEDTAMG VEEVMAVKKG SVANSSSYMG YGAGGGGANQ LQPSQQIPIT
     DLKSALRATD LLIGELGGFR SSTGATPQNF TRSFTTLTPQ QFKHQLISHT NAMAIAAQGL
     FQDMTTPPPT GGIAAFQQAI TKRAQIIMAE LNTVGTAAKN AGYFPDMASF SDEIIGVATK
     LSESMARLLA IGSTIQGTDC DEKSQKAAQT EIFNVQSLVT LMMAACDNEY VTDSSSKLLI
     ECAKNVSAAI ADMLVVGNSK VEFIDDELLL GQIQNTLKST SLTSDELLST TENLASTSCH
     PESRKQITNI TQSALNQSNA LLTAFKSGEI PEQDYNLLNA RVSDIIESVN LINYAMDCSE
     REYKISITSN GVEVGEGEIL AGTNLTEEFA TVANDLTNAI MTMRSNLKNP DTVMESYKMV
     AGHANRLITC TKAVASRADT QSQQRLFNST NAVFESVANL SNHCRSYIKN PEQEAHTFQI
     VETAGHLQFL TQNMSTDAGK IACITSLRDY SKEMIAQVSS LISTSRTSSQ YLPDANGITL
     LKGAKDVSDA LSKLMVGIKK VVLDPKSEAT QMELLTLAQK QSLPPMNLVS TCKRFAPKIS
     DPNQKQRLIF SSDAAAQSVQ KLMKAGEAYK RICGHIEIEE ALEVFDSTIA DLETTEIAIA
     GGFLDAVSGT TREGAAELLM VAIKDLNKVN NELVTDIRVN PARLGDLVKS ATESASSVAI
     SAKTLICATT GKQVQTKLMG ITKQLMIDME QLIRASRSVR SNPNDRRSEL LLDRRSNDVS
     ISTAALVGST ANVDCKELDE ASADISNLLS LKMGSLESIL SQPTEEFAFY VEEIASSTKA
     LNAASQQVVA MARNKNLKGL GASAKITASA LSTLVSHAQN AIVLTENEAT KNAILASTVA
     LGGQIIGLLD FSKARIANYK DPIYDQNLIN QAKSVEDHLV KVGRSLGGDG NNTICDEAVD
     RIIEATRSLD KTILPDTSGL QTNAHLEMLH QQSLLAITQA SKKLGSITSN LVNSKNNSDL
     VGSGSTDAER IIEMIEAAKH VVHCSISTYN PDILLPAKSI LDASQMLTAN QADVNHVLSH
     AATIAACTQQ LLGITRERAS QFNEQDEQQV QVRDGIVKST QQLAHATSSL ARAVKSVTSK
     EPGAKAMISQ SLKDLESAIN NLLITSSVPA SERGIGIADF NKLMSTCRSV STASSQLIIS
     ASSCSQKPKD IELSSILSEN AVLMTNSLKD IIKVTSSMMP GVNFCEEAIE IAQRAISDLS
     SVALSVAVGS FDSSANNKEG LSHVESQERL VDVTKKIGTG INDLLKASRQ SPEAIGISAK
     ALSFIAPSLV NTTKPALATA PDADAQNDLV TESKNVGDSI LKLCQASLIA SSNPSKETYQ
     IIVNKCVDAS EAMSKLVAQI SSGVNLYKEL DESLDRIRKS VVQTSAKDAP KDSENRGYQE
     YKEELSNLTK NLALSLKTIV ATDGNNLVSI STISKDIANY ISDIAHVSSA ILATTSDQKI
     RDSIITSSRQ VIVSTGDIVN HIKVNSTDKA NSSQAKVNDS YRATNDNITR FLQSLKQGAI
     GEILSDAAID QIRKVISDLD GYSLFAAAGQ LENDQSSQST MNEVTKQQHL KNLQKDTITQ
     AKLLIVSSSQ LVGSSRGTQE HLGSATTKVA NTVSSLVKTA KDIASVLADT TSQQDILSAS
     KALSISSQQM VLATKDAQRF KKDATAFRSL GKSAEAVAEA VGQFLTSVYT AISDAGKGIK
     ELEKSIVQVA NYHEKPDTVL SNKDATAEIF AQSARDLAKS SIEIVTSYTS SQDSLVKSSQ
     AVVSNVQSFI SNSKGVIALL GNGNDDLKSK VLENVKQTTG DMLALLQCVK DQDKNGSTSI
     ADATRSISDR VHSVVTLSKS LPGGQNIVVE EDNVLEDLEA LAEDELSACA RSIEEATAKL
     IAARPQSKSK NGKLDAEGVA ATIVDASSAI AKAVAKLVNS AAVAQSKRRE DQIASGSVYK
     ADPTWSNGLI SAAKGVGAAT HRLVEAAMKS ATGKAEEEEL IATARSVAAA TALLVSASRA
     KSGDDYQSQA AHSHLSTAAR QVASATSDLV AAAKAATIFD EQQQEEEQEQ FNFTGSKVKE
     LEQQMKILKL EKELETARRQ MLNSRKQNYN KN