ID   TALD3_DANRE             Reviewed;        1554 AA.
AC   H6D7E6; F1R2W6;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2012, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=Protein TALPID3;
GN   Name=talpid3;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION,
RP   DISRUPTION PHENOTYPE, AND FUNCTION.
RC   STRAIN=AB, and Singapore;
RX   PubMed=22028029; DOI=10.1242/dev.070862;
RA   Ben J., Elworthy S., Ng A.S., van Eeden F., Ingham P.W.;
RT   "Targeted mutation of the talpid3 gene in zebrafish reveals its conserved
RT   requirement for ciliogenesis and Hedgehog signalling across the
RT   vertebrates.";
RL   Development 138:4969-4978(2011).
CC   -!- FUNCTION: Required for ciliogenesis and sonic hedgehog/SHH signaling.
CC       {ECO:0000269|PubMed:22028029}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body
CC       {ECO:0000269|PubMed:22028029}. Cytoplasm, cytoskeleton, microtubule
CC       organizing center, centrosome, centriole {ECO:0000269|PubMed:22028029}.
CC   -!- DEVELOPMENTAL STAGE: Ubiquitously distributed in embryos from early
CC       cleavage stages through to 2 dpf. {ECO:0000269|PubMed:22028029}.
CC   -!- DISRUPTION PHENOTYPE: Homozygous for these mutant alleles complete
CC       embryogenesis normally, but manifest a cystic kidney phenotype during
CC       the early larval stages and die within a month of hatching. Elimination
CC       of maternally derived talpid3 activity by germline replacement resulted
CC       in embryonic lethality of talpid3 homozygotes. The phenotype of such
CC       maternal and zygotic mutant show absence of primary and motile cilia as
CC       well as aberrant Hedgehog signaling. {ECO:0000269|PubMed:22028029}.
CC   -!- SIMILARITY: Belongs to the TALPID3 family. {ECO:0000305}.
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DR   EMBL; JN088213; AEH76002.1; -; mRNA.
DR   RefSeq; NP_001245157.1; NM_001258228.1.
DR   AlphaFoldDB; H6D7E6; -.
DR   SMR; H6D7E6; -.
DR   STRING; 7955.ENSDARP00000129806; -.
DR   PaxDb; H6D7E6; -.
DR   GeneID; 100884142; -.
DR   KEGG; dre:100884142; -.
DR   ZFIN; ZDB-GENE-120119-1; si:ch211-185a18.2.
DR   eggNOG; ENOG502QUXJ; Eukaryota.
DR   InParanoid; H6D7E6; -.
DR   OrthoDB; 66287at2759; -.
DR   PRO; PR:H6D7E6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR   GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0060271; P:cilium assembly; IMP:ZFIN.
DR   GO; GO:0035845; P:photoreceptor cell outer segment organization; IMP:ZFIN.
DR   GO; GO:0007224; P:smoothened signaling pathway; IMP:ZFIN.
DR   InterPro; IPR029246; TALPID3.
DR   PANTHER; PTHR15721; PTHR15721; 1.
DR   Pfam; PF15324; TALPID3; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium biogenesis/degradation; Coiled coil; Cytoplasm;
KW   Cytoskeleton; Reference proteome.
FT   CHAIN           1..1554
FT                   /note="Protein TALPID3"
FT                   /id="PRO_0000420183"
FT   REGION          57..84
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          227..247
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          312..396
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          411..466
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          489..523
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          553..639
FT                   /note="Required for centrosomal localization"
FT                   /evidence="ECO:0000250"
FT   REGION          639..687
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1079..1178
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1214..1265
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1354..1374
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1500..1554
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          204..270
FT                   /evidence="ECO:0000255"
FT   COILED          556..586
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        358..396
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        412..466
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        493..508
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1079..1098
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1099..1113
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1119..1134
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1147..1173
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1226..1240
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1241..1265
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1529..1554
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1554 AA;  167601 MW;  E45FBDFACE8F41EE CRC64;
     MDSCCVSVNN KTHFNDSTSS SDAADVLIHS TRAGRDEIQD KSQNKVNIYI RRLSDSEHVH
     SADERRTEIP PVNLPTASDT AGPEEPLAGI KSNQFAGLFG KTSRKEKSKA QSTIMQTEQL
     KSKGEDVQIS TFSADGRGVV SAALRKRSQG APIRRNVTVQ VLDQDRSQTP AGVQPDPAFP
     LGDPGTSASV AAITAATLAA TAPLMKAQSE MEAQISRVSA ELKRLQAAEG SVPPGRTART
     DSSSAGRAAH LEEQLNILIQ QRLQHLETIQ CQQIQLQNRL LGSALDVVAS RGNSSGVSQT
     DSLPLQISGN RSIRLSATDN PSAGRPASVS MDVYRERQTG GHKSPLETPA PRKVIPKPTH
     WTSSTSTNRP PKSSFKNQGN GRLQDQSPNN RRSPERCALQ SVGVERFAMA TADNSQPEQS
     RESQMPSKAS VTRSAQEDGS SFVNGQNEQE RQGEPTNVSS SSTTVQKASE MLQDLGRLKN
     EMRSLLQTAD AFPVPNAKST QSSRSRHLAP VATAPPPATA PISMPPEPVD VIAVRAAALN
     RASVLKSIQP PTSMFEDAGL VLRQVRQSKK TLEENLEAIL RAKDGEVLHT QLEALSKNRD
     VREELRIKKT VDAWINTLSK EIQDDLARES SERIVDAAVS RREAGQRTRA EASAAAKKTS
     VRAQAGNTRA HTRQPAPAVR NKPAVKQEAT QPIVLKSQDD EEYLARLYGK AIYDGQRRTL
     KKSPYLRFNS PTPKSKPQRP KIVETVKGVK MKSSKTQTSQ YVGDVSAMQH SVSEPHFIFS
     PSDPDKQQQQ PGSPVRGYLI PMAIPLGKPR VDCQPPVPSR VIITDKPAIV TTSFPPITVV
     ESKPTPAIRK PNAILLEVQS APKKRTPQLQ IQVQPGVNIE SALCSSRQAS PTPVIPAESI
     LPPPSIHATE DPHAHEDQQE NIFPGTNFLA QADISQETNG GLPDSPIEFK GLPSPPADLY
     HGPVFPPVPT QSTPLTEPIL NTIQQRETLE NRLVDWVEQQ IMARVITGMF PQPAQADPVH
     QSEPENSVAS DIVEAAGGSG LQLFVDTGVP VDSEVIRHYV NEVLTEIIAS MLGQREAQGT
     PATLVQTQDA QKEDTTVPTP APTPEPSLKD PPSPVRTPDL SEHLSTATSP EKPPQESASP
     GPDRIPVGTP ITTPIPSPTR VATPSPPTPA NQSPEPGSLQ IHLWEGSELP LEEEEQPELQ
     PAPVVISVPR VDDQEESVIH PSSPVLSKPQ SPPAPPLPPV IQKSESSSSS SSSSSESSCS
     SSVTVTETET AARHISEGEL LLNHGQMAAV RVLEQEGVLL PNFMTSLNGS LHGVQDMDYD
     PPSEGQVIRA PHLPAHHDPV LSLLARMELG PISQSQQPEG WWEEESSGEV SEGQRPVLTA
     AEEIVLTGHS LMDQQTIRQS RNTQISPHAT LTSPGQVIAE HTGAVVEDGG LSVNLRSSDE
     QKEAMVYQAD TVSSPQPESS QMAQTVHRPA PILVRQYEEE PDFPQLRRLS DDAFFGADEK
     GEDTFLHTGE GGGGGRGSDV RVMSVRLPSV KQDQESVSLS SVEGDTDSSA NDVF