ID   BPP7A_BOTJA             Reviewed;           7 AA.
AC   P85160;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2007, sequence version 1.
DT   26-FEB-2020, entry version 25.
DE   RecName: Full=Bradykinin-potentiating peptide 7a;
DE            Short=BPP-7a;
DE   AltName: Full=Proline-rich peptide 7a;
DE            Short=BjPRO-7a;
DE            Short=PRO-7a;
OS   Bothrops jararaca (Jararaca) (Bothrops jajaraca).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Bothrops.
OX   NCBI_TaxID=8724;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP   SPECTROMETRY, AND PYROGLUTAMATE FORMATION AT GLN-1.
RC   TISSUE=Venom {ECO:0000269|PubMed:15245866};
RX   PubMed=15245866; DOI=10.1016/j.peptides.2004.04.006;
RA   Ianzer D., Konno K., Marques-Porto R., Portaro F.C.V., Stoecklin R.,
RA   de Camargo A.C.M., Pimenta D.C.;
RT   "Identification of five new bradykinin potentiating peptides (BPPs) from
RT   Bothrops jararaca crude venom by using electrospray ionization tandem mass
RT   spectrometry after a two-step liquid chromatography.";
RL   Peptides 25:1085-1092(2004).
RN   [2]
RP   FUNCTION.
RX   PubMed=17475904; DOI=10.1124/jpet.107.120873;
RA   Ianzer D., Santos R.A., Etelvino G.M., Xavier C.H., de Almeida Santos J.,
RA   Mendes E.P., Machado L.T., Prezoto B.C., Dive V., de Camargo A.C.;
RT   "Do the cardiovascular effects of angiotensin-converting enzyme (ACE) I
RT   involve ACE-independent mechanisms? new insights from proline-rich peptides
RT   of Bothrops jararaca.";
RL   J. Pharmacol. Exp. Ther. 322:795-805(2007).
RN   [3]
RP   SYNTHESIS, AND FUNCTION.
RX   PubMed=20814884; DOI=10.1002/cyto.a.20963;
RA   Negraes P.D., Lameu C., Hayashi M.A., Melo R.L., Camargo A.C., Ulrich H.;
RT   "The snake venom peptide Bj-PRO-7a is a M1 muscarinic acetylcholine
RT   receptor agonist.";
RL   Cytometry A 79:77-83(2011).
CC   -!- FUNCTION: This peptide shows potent and long-lasting antihypertensive
CC       activity as well as a reduction of the heart rate. These effects may be
CC       explained by the agonistic activities of this peptide for the
CC       muscarinic acetylcholine receptor M1 (CHRM1) (EC(50)=0.25-0.31 uM),
CC       since these types of G-protein coupled receptor have been demonstrated
CC       to be active in the blood pressure control.
CC       {ECO:0000269|PubMed:15245866, ECO:0000269|PubMed:17475904,
CC       ECO:0000269|PubMed:20814884}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15245866}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:15245866}.
CC   -!- MASS SPECTROMETRY: Mass=705.8; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:15245866};
CC   -!- MISCELLANEOUS: This peptide weakly inhibits angiotensin-converting
CC       enzyme (ACE) with no clear preference for either C- or N-domains. It
CC       does not potentiate bradykinin, and does not affect angiotensin-1
CC       pressor effects. It does not bind to type-1 angiotensin-2 receptor
CC       (AGTR1) (PubMed:17475904). It does not bind to the muscarinic
CC       acetylcholine receptor M3 (CHRM3) (PubMed:20814884).
CC       {ECO:0000305|PubMed:17475904, ECO:0000305|PubMed:20814884}.
CC   -!- SIMILARITY: Belongs to the bradykinin-potentiating peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0008217; P:regulation of blood pressure; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing;
KW   G-protein coupled acetylcholine receptor impairing toxin;
KW   G-protein coupled receptor impairing toxin; Hypotensive agent;
KW   Pyrrolidone carboxylic acid; Secreted; Toxin.
FT   PEPTIDE         1..7
FT                   /note="Bradykinin-potentiating peptide 7a"
FT                   /evidence="ECO:0000269|PubMed:15245866"
FT                   /id="PRO_0000292914"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:15245866"
SQ   SEQUENCE   7 AA;  723 MW;  77776047687AB6B0 CRC64;
     QDGPIPP