ID   TAL_KITKI               Reviewed;         318 AA.
AC   E5F146;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=dTDP-6-deoxy-L-talose 4-dehydrogenase (NAD(P)(+));
DE            EC=1.1.1.344;
GN   Name=tal;
OS   Kitasatospora kifunensis (Streptomyces kifunensis).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Kitasatospora.
OX   NCBI_TaxID=58351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=MJM341;
RX   PubMed=20667525; DOI=10.1016/j.carres.2010.07.004;
RA   Karki S., Yoo H.G., Kwon S.Y., Suh J.W., Kwon H.J.;
RT   "Cloning and in vitro characterization of dTDP-6-deoxy-L-talose
RT   biosynthetic genes from Kitasatospora kifunensis featuring the dTDP-6-
RT   deoxy-L-lyxo-4-hexulose reductase that synthesizes dTDP-6-deoxy-L-talose.";
RL   Carbohydr. Res. 345:1958-1962(2010).
CC   -!- FUNCTION: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to
CC       dTDP-6-deoxy-L-talose. Can use NAD(+) or NADP(+).
CC       {ECO:0000269|PubMed:20667525}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dTDP-6-deoxy-beta-L-talose + NAD(+) = dTDP-4-dehydro-beta-L-
CC         rhamnose + H(+) + NADH; Xref=Rhea:RHEA:34447, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62830,
CC         ChEBI:CHEBI:68576; EC=1.1.1.344;
CC         Evidence={ECO:0000269|PubMed:20667525};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dTDP-6-deoxy-beta-L-talose + NADP(+) = dTDP-4-dehydro-beta-L-
CC         rhamnose + H(+) + NADPH; Xref=Rhea:RHEA:23648, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62830,
CC         ChEBI:CHEBI:68576; EC=1.1.1.344;
CC         Evidence={ECO:0000269|PubMed:20667525};
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. {ECO:0000305}.
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DR   EMBL; HM132058; ADO32602.1; -; Genomic_DNA.
DR   AlphaFoldDB; E5F146; -.
DR   SMR; E5F146; -.
DR   KEGG; ag:ADO32602; -.
DR   BRENDA; 1.1.1.344; 12319.
DR   GO; GO:0047003; F:dTDP-6-deoxy-L-talose 4-dehydrogenase activity; IEA:RHEA.
DR   InterPro; IPR001509; Epimerase_deHydtase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF01370; Epimerase; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   NAD; NADP; Oxidoreductase.
FT   CHAIN           1..318
FT                   /note="dTDP-6-deoxy-L-talose 4-dehydrogenase (NAD(P)(+))"
FT                   /id="PRO_0000424196"
FT   ACT_SITE        145
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         19..20
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         60..61
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         95
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         120
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         120
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         145
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         145
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         149
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   318 AA;  33102 MW;  E48F5DB98D1403E4 CRC64;
     MTAPHTAPAR VAVLGGTGFI GRVLGARLLA QGAEVLSLAR KAPAEPAPGR FVAFDLSNGD
     PAELTALLDR ERIDTVVNAA GGMWGLNDEQ MYQANVVLTE RLIEAVAAMA SPARLVHLGT
     VHEYGMAPVG TSQRESDPAA PVMEYGKLKL AATEAVVRAV EAGRISGVVL RLGNVVGAGQ
     PGHSLLGVMA AKLDAARAAG ETAQLSLQPL TALRDFVDLT DTLDAVLLAA ADRSAPPVVN
     VGTGSASTAR HLVELLIEES GVPTEITEVP APDGTGPETE WQQLDVTVAR DSLGWTPRRT
     LREAVRELWT AQSTAPVA