ID   BPPAE_BOTCO             Reviewed;          10 AA.
AC   P0DJK3;
DT   03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   29-SEP-2021, entry version 15.
DE   RecName: Full=Bradykinin-potentiating peptide 10e;
DE            Short=BPP-10e;
OS   Bothrops cotiara (Cotiara) (Rhinocerophis cotiara).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Bothrops.
OX   NCBI_TaxID=8727;
RN   [1]
RP   PROTEIN SEQUENCE, SYNTHESIS, FUNCTION, PYROGLUTAMATE FORMATION AT GLN-1,
RP   AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=22869554; DOI=10.1074/mcp.m112.019331;
RA   Tashima A.K., Zelanis A., Kitano E.S., Ianzer D., Melo R.L., Rioli V.,
RA   Sant'anna S.S., Schenberg A.C., Camargo A.C., Serrano S.M.T.;
RT   "Peptidomics of three Bothrops snake venoms: insights into the molecular
RT   diversification of proteomes and peptidomes.";
RL   Mol. Cell. Proteomics 11:1245-1262(2012).
CC   -!- FUNCTION: This peptide evokes transient hypotension (-11 mmHg) similar
CC       to that evoked by 0,5 ug of bradykinin, when injected alone into rats.
CC       It has a high bradykinin-potentiating effect (113%), when 60 nmol of
CC       BPP-10e are coinjected with 0.5 ug of bradykinin into rats. It inhibits
CC       angiotensin converting enzyme (ACE) activity with a K(i)app of 16.21
CC       uM. {ECO:0000269|PubMed:22869554}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MASS SPECTROMETRY: Mass=1131.5; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:22869554};
CC   -!- SIMILARITY: Belongs to the bradykinin-potentiating peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008191; F:metalloendopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0008217; P:regulation of blood pressure; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hypotensive agent; Metalloenzyme inhibitor;
KW   Metalloprotease inhibitor; Protease inhibitor; Pyrrolidone carboxylic acid;
KW   Secreted; Toxin.
FT   PEPTIDE         1..10
FT                   /note="Bradykinin-potentiating peptide 10e"
FT                   /id="PRO_0000421904"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:22869554"
FT   UNSURE          7
FT                   /note="K or Q"
SQ   SEQUENCE   10 AA;  1149 MW;  30C527C32775A773 CRC64;
     QNWPSPKVPP