TAMA_CITRI
ID TAMA_CITRI Reviewed; 577 AA.
AC D2TN56;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 02-MAR-2010, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=Translocation and assembly module subunit TamA;
DE AltName: Full=Autotransporter assembly factor TamA;
DE Flags: Precursor;
GN Name=tamA; OrderedLocusNames=ROD_32811;
OS Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Citrobacter.
OX NCBI_TaxID=637910;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ICC168;
RX PubMed=19897651; DOI=10.1128/jb.01144-09;
RA Petty N.K., Bulgin R., Crepin V.F., Cerdeno-Tarraga A.M., Schroeder G.N.,
RA Quail M.A., Lennard N., Corton C., Barron A., Clark L., Toribio A.L.,
RA Parkhill J., Dougan G., Frankel G., Thomson N.R.;
RT "The Citrobacter rodentium genome sequence reveals convergent evolution
RT with human pathogenic Escherichia coli.";
RL J. Bacteriol. 192:525-538(2010).
RN [2]
RP FUNCTION IN PROTEIN TRANSLOCATION, SUBUNIT, SUBCELLULAR LOCATION,
RP INTERACTION WITH TAMB, AND DISRUPTION PHENOTYPE.
RC STRAIN=ICC169;
RX PubMed=22466966; DOI=10.1038/nsmb.2261;
RA Selkrig J., Mosbahi K., Webb C.T., Belousoff M.J., Perry A.J., Wells T.J.,
RA Morris F., Leyton D.L., Totsika M., Phan M.D., Celik N., Kelly M.,
RA Oates C., Hartland E.L., Robins-Browne R.M., Ramarathinam S.H.,
RA Purcell A.W., Schembri M.A., Strugnell R.A., Henderson I.R., Walker D.,
RA Lithgow T.;
RT "Discovery of an archetypal protein transport system in bacterial outer
RT membranes.";
RL Nat. Struct. Mol. Biol. 19:506-510(2012).
CC -!- FUNCTION: Part of the translocation and assembly module (TAM)
CC autotransporter assembly complex, which functions in translocation of
CC autotransporters across the outer membrane.
CC {ECO:0000269|PubMed:22466966}.
CC -!- SUBUNIT: Interacts with TamB. {ECO:0000250|UniProtKB:P0ADE4}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000269|PubMed:22466966}.
CC -!- DOMAIN: Contains 3 N-terminal periplasmic polypeptide transport-
CC associated (POTRA) domains and a C-terminal 16-stranded beta-barrel
CC transmembrane region. The beta-barrel is closed on the extracellular
CC face by loop 6 (residues 456-495), while the C-terminal beta-strand
CC forms an inward kink which may act as a gate for substrate access from
CC the periplasm. This kink weakens the beta-strand pair between the first
CC and last strands which may contribute to gating and substrate
CC translocation. {ECO:0000250|UniProtKB:P0ADE4}.
CC -!- DISRUPTION PHENOTYPE: 8-fold reduction in ability to colonize mice in
CC competitive assays with wild-type. Loss of localization of P1121 to
CC outer membranes. {ECO:0000269|PubMed:22466966}.
CC -!- SIMILARITY: Belongs to the TamA family. {ECO:0000305}.
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DR EMBL; FN543502; CBG90000.1; -; Genomic_DNA.
DR RefSeq; WP_012907368.1; NC_013716.1.
DR AlphaFoldDB; D2TN56; -.
DR SMR; D2TN56; -.
DR DIP; DIP-59929N; -.
DR IntAct; D2TN56; 1.
DR STRING; 637910.ROD_32811; -.
DR KEGG; cro:ROD_32811; -.
DR eggNOG; COG0729; Bacteria.
DR HOGENOM; CLU_018618_1_0_6; -.
DR OMA; NTTMLLY; -.
DR OrthoDB; 470487at2; -.
DR Proteomes; UP000001889; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR000184; Bac_surfAg_D15.
DR InterPro; IPR010827; BamA/TamA_POTRA.
DR InterPro; IPR039910; D15-like.
DR InterPro; IPR034746; POTRA.
DR InterPro; IPR035243; TamA_POTRA_Dom_1.
DR PANTHER; PTHR12815; PTHR12815; 1.
DR Pfam; PF01103; Omp85; 1.
DR Pfam; PF07244; POTRA; 1.
DR Pfam; PF17243; POTRA_TamA_1; 1.
DR PROSITE; PS51779; POTRA; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane beta strand; Virulence.
FT SIGNAL 1..21
FT /evidence="ECO:0000305"
FT CHAIN 22..577
FT /note="Translocation and assembly module subunit TamA"
FT /id="PRO_5000565833"
FT TRANSMEM 265..276
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 279..288
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 297..306
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 309..317
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 327..336
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 347..356
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 361..370
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 387..395
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 406..415
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 428..437
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 444..453
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 500..508
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 514..523
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 536..545
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 549..557
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 568..577
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT DOMAIN 187..263
FT /note="POTRA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
SQ SEQUENCE 577 AA; 64851 MW; 098D65676266735D CRC64;
MPHIRQLCWV SLMCLSSSAF AANVRLQVEG LSGELERNVR AQLSTIQSDE VTPDRRFRAR
VDDAIREGLK ALGYYEPTID FDLRPPPAKG RQVLLARVSP GEPVRIGGTG VILRGGARTD
RDYLDLLKTR PAIGTVLNHG DYDNFKKSLT SVALRKGYFD SEFNKSQLGV ALDRHQAFWD
IDYNSGERYR FGHVTFEGSQ IRDEYLQNLV PFKEGDEYES KDLGELNRRL SATGWFNSVV
VAPEFDKARE TKVLPLKGVV SPRTENTVET GVGYSTDVGP RVKATWKKPW MNSYGHSLTT
SASISAPEQV LDFSYKIPLL KNPLEQYYLV QGGFKRTDLN DTEQDSTTLA LSRYWDLSSG
WQRAINLRWS LDHFTQGEVT NTTMLLYPGV MISRTRSRGG LMPTWGDSQR YSVDYSNTAW
GSDVDFSVIQ AQNVWIRTLY DRHRFVMRGN LGWIETGDFD KVPPDLRFFA GGDRSIRGYK
YKSISPKDSN GDLKGASKLA TGSLEYQYNV TGKWWGAVFV DSGEAVSDIR RSDFKTGAGV
GVRWQSPVGP IKLDFAAPIG DKDEHGLQFY IGLGPEL
