TAMA_ECO57
ID TAMA_ECO57 Reviewed; 577 AA.
AC P0ADE5; P39320;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Translocation and assembly module subunit TamA;
DE AltName: Full=Autotransporter assembly factor TamA;
DE Flags: Precursor;
GN Name=tamA; OrderedLocusNames=Z5831, ECs5198;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Part of the translocation and assembly module (TAM)
CC autotransporter assembly complex, which functions in translocation of
CC autotransporters across the outer membrane. Has anion selective
CC channel-forming ability, but the physiological relevance of this
CC activity is unclear. {ECO:0000250|UniProtKB:P0ADE4}.
CC -!- SUBUNIT: Interacts with TamB. {ECO:0000250|UniProtKB:P0ADE4}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000250|UniProtKB:P0ADE4}.
CC -!- DOMAIN: Contains 3 N-terminal periplasmic polypeptide transport-
CC associated (POTRA) domains and a C-terminal 16-stranded beta-barrel
CC transmembrane region. The beta-barrel is closed on the extracellular
CC face by loop 6 (residues 456-495), while the C-terminal beta-strand
CC forms an inward kink which may act as a gate for substrate access from
CC the periplasm. This kink weakens the beta-strand pair between the first
CC and last strands which may contribute to gating and substrate
CC translocation. {ECO:0000250|UniProtKB:P0ADE4}.
CC -!- SIMILARITY: Belongs to the TamA family. {ECO:0000305}.
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DR EMBL; AE005174; AAG59418.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB38621.1; -; Genomic_DNA.
DR PIR; F91278; F91278.
DR RefSeq; NP_313225.1; NC_002695.1.
DR RefSeq; WP_001269327.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0ADE5; -.
DR BMRB; P0ADE5; -.
DR SMR; P0ADE5; -.
DR STRING; 155864.EDL933_5568; -.
DR EnsemblBacteria; AAG59418; AAG59418; Z5831.
DR EnsemblBacteria; BAB38621; BAB38621; ECs_5198.
DR GeneID; 66671861; -.
DR GeneID; 913933; -.
DR KEGG; ece:Z5831; -.
DR KEGG; ecs:ECs_5198; -.
DR PATRIC; fig|386585.9.peg.5435; -.
DR eggNOG; COG0729; Bacteria.
DR HOGENOM; CLU_018618_1_0_6; -.
DR OMA; NTTMLLY; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR000184; Bac_surfAg_D15.
DR InterPro; IPR010827; BamA/TamA_POTRA.
DR InterPro; IPR039910; D15-like.
DR InterPro; IPR034746; POTRA.
DR InterPro; IPR035243; TamA_POTRA_Dom_1.
DR PANTHER; PTHR12815; PTHR12815; 1.
DR Pfam; PF01103; Omp85; 1.
DR Pfam; PF07244; POTRA; 1.
DR Pfam; PF17243; POTRA_TamA_1; 1.
DR PROSITE; PS51779; POTRA; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane beta strand.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..577
FT /note="Translocation and assembly module subunit TamA"
FT /id="PRO_0000043227"
FT TRANSMEM 265..276
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 279..288
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 297..306
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 309..317
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 327..336
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 347..356
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 361..370
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 387..395
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 406..415
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 428..437
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 444..453
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 500..508
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 514..523
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 536..545
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 549..557
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT TRANSMEM 568..577
FT /note="Beta stranded"
FT /evidence="ECO:0000250|UniProtKB:P0ADE4"
FT DOMAIN 187..263
FT /note="POTRA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
SQ SEQUENCE 577 AA; 64796 MW; 253D1D5BEA744D25 CRC64;
MRYIRQLCCV SLLCLSGSAV AANVRLQVEG LSGQLEKNVR AQLSTIESDE VTPDRRFRAR
VDDAIREGLK ALGYYQPTIE FDLRPPPKKG RQVLIAKVTP GVPVLIGGTD VVLRGGARTD
KDYLKLLDTR PAIGTVLNQG DYENFKKSLT SIALRKGYFD SEFTKAQLGI ALGLHKAFWD
IDYNSGERYR FGHVTFEGSQ IRDEYLQNLV PFKEGDEYES KDLAELNRRL SATGWFNSVV
VAPQFDKARE TKVLPLTGVV SPRTENTIET GVGYSTDVGP RVKATWKKPW MNSYGHSLTT
STSISAPEQT LDFSYKMPLL KNPLEQYYLV QGGFKRTDLN DTESDSTTLV ASRYWDLSSG
WQRAINLRWS LDHFTQGEIT NTTMLFYPGV MISRTRSRGG LMPTWGDSQR YSIDYSNTAW
GSDVDFSVFQ AQNVWIRTLY DRHRFVTRGT LGWIETGDFD KVPPDLRFFA GGDRSIRGYK
YKSIAPKYAN GDLKGASKLI TGSLEYQYNV TGKWWGAVFV DSGEAVSDIR RSDFKTGTGV
GVRWESPVGP IKLDFAVPVA DKDEHGLQFY IGLGPEL
