ID   TAMB_SALTS              Reviewed;        1259 AA.
AC   E1WAU5;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 44.
DE   RecName: Full=Translocation and assembly module subunit TamB;
DE   AltName: Full=Autotransporter assembly factor TamB;
GN   Name=tamB; OrderedLocusNames=SL1344_4343;
OS   Salmonella typhimurium (strain SL1344).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=216597;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SL1344;
RX   PubMed=22538806; DOI=10.1073/pnas.1201061109;
RA   Kroger C., Dillon S.C., Cameron A.D., Papenfort K., Sivasankaran S.K.,
RA   Hokamp K., Chao Y., Sittka A., Hebrard M., Handler K., Colgan A.,
RA   Leekitcharoenphon P., Langridge G.C., Lohan A.J., Loftus B., Lucchini S.,
RA   Ussery D.W., Dorman C.J., Thomson N.R., Vogel J., Hinton J.C.;
RT   "The transcriptional landscape and small RNAs of Salmonella enterica
RT   serovar Typhimurium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:E1277-E1286(2012).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=SL1344;
RX   PubMed=22466966; DOI=10.1038/nsmb.2261;
RA   Selkrig J., Mosbahi K., Webb C.T., Belousoff M.J., Perry A.J., Wells T.J.,
RA   Morris F., Leyton D.L., Totsika M., Phan M.D., Celik N., Kelly M.,
RA   Oates C., Hartland E.L., Robins-Browne R.M., Ramarathinam S.H.,
RA   Purcell A.W., Schembri M.A., Strugnell R.A., Henderson I.R., Walker D.,
RA   Lithgow T.;
RT   "Discovery of an archetypal protein transport system in bacterial outer
RT   membranes.";
RL   Nat. Struct. Mol. Biol. 19:506-510(2012).
CC   -!- FUNCTION: Part of the translocation and assembly module (TAM)
CC       autotransporter assembly complex, which functions in translocation of
CC       autotransporters across the outer membrane.Substrate binding to TamA
CC       moves its POTRA domains about 30 Angstroms into the periplasm, which
CC       would deform either the outer membrane or TamB and may provide force to
CC       reset TAM. {ECO:0000250|UniProtKB:P39321}.
CC   -!- SUBUNIT: Interacts with TamA. {ECO:0000250|UniProtKB:P39321}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P39321}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:P39321}.
CC   -!- DOMAIN: The periplasmic domain is elongated (up to 160 Angstroms long)
CC       and forms contacts to the N-terminal POTRA domains of TamA.
CC       {ECO:0000250|UniProtKB:P39321}.
CC   -!- DISRUPTION PHENOTYPE: Significantly reduced survival in human serum,
CC       probably due to complement-mediated killing.
CC       {ECO:0000269|PubMed:22466966}.
CC   -!- SIMILARITY: Belongs to the TamB family. {ECO:0000305}.
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DR   EMBL; FQ312003; CBW20429.1; -; Genomic_DNA.
DR   RefSeq; WP_000060815.1; NZ_QASL01000004.1.
DR   AlphaFoldDB; E1WAU5; -.
DR   SMR; E1WAU5; -.
DR   EnsemblBacteria; CBW20429; CBW20429; SL1344_4343.
DR   KEGG; sey:SL1344_4343; -.
DR   PATRIC; fig|216597.6.peg.4836; -.
DR   HOGENOM; CLU_002338_0_1_6; -.
DR   OMA; NTAKQWP; -.
DR   BioCyc; SENT216597:SL1344_RS22635-MON; -.
DR   Proteomes; UP000008962; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   InterPro; IPR007452; TamB.
DR   Pfam; PF04357; TamB; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..1259
FT                   /note="Translocation and assembly module subunit TamB"
FT                   /id="PRO_0000418090"
FT   TOPO_DOM        1..6
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        7..27
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        28..1259
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1259 AA;  136223 MW;  0B0E4D52DF7B7F3B CRC64;
     MSLWKKISLG VLIFIVVLLA SVAFLVGTTT GLHLVFSAAN RWVPGLEIGQ VTGGWRDLSL
     KNIRYEQPGV AVNAGEIHLA IGLDCLWRSS LCVNDLALKD INVAIDSKKM PPSEPAQEEE
     ESGPLNLSTP WPITLSRVAL NNINIKIDDT TVSVLDFTSG LAWQEKNLTL KPTRLQGLLI
     ALPKVADVAQ EEVVEPKIEK PQPDEKPLGE TLKDLFAKPV MPEMTDVHLP LNLNIESFRG
     EQLRITGDTD LTVRTMLLKV SSIDGNMKLD TLDIDANQGT VKASGTAQLA NNWPVDITLN
     STLNIDPLKG EKIKLKVGGA LREQLEVGVN LSGPMDVALR AQTRLAEAGL PLNLEVVSQR
     IAWPLTGDTQ FQADDLKLKL SGKMTDYTLS MRTAVKGQDI PPATITLDAK GNERQINLDK
     LTVAALEGKT ELKALVDWQQ AISWRGELTL NGINTAKEIP DWPAKLNGVM KTKGSLYGGT
     WQMDVPELKL TGNVKQNSVN VNGTLKGNSY MQWTIPGLHF ALGPNSADIK GELGVKELNL
     DAAIDAPGLD NALPGLGGMA KGIVKVRGTV EAPQLLADIT ARGLRWQELS VAQARIEGDI
     KSTDQIAGHL NVRVERISQP DVNINLVTLD AKGSEKQHQL QLRVQGEPVS GQLSLTGSFD
     REAARWKGTL SDTRFQTPVG PWSLTRAIAL DYRNKEQKIS IGPHCWLNPN AELCIPQTID
     AGAAGRAVVN LNRFDLAMLK PFMPDTTQAS GIFSGKADVS WDTTQEGLPQ GKVTLSGRNV
     KVTQTVNDAP LPVAFETLNL SADLHNNRAE LGWLIRLTNN GQFDGQVQVT DPQGRRNLGG
     NVNMRNLNLA MVNPVFSRGE KAAGMLNARL RLGGDVQSPQ LFGQLQLSAL DIDGNFMPFE
     MQPSQLTMNF SGTRSTLAGI VRTQQGQINL NGNADWSQID NWRARVTAKG SRVRITVPPM
     VRLDVSPDVV FDATPSLFTL DGRVDVPWAR IVVHDLPESA VGVSSDVVML NNDLQPETPQ
     TASIPINSNL TVHVGNNVRI DAFGLKARLT GDLKVAQDKQ GLGLNGQINI PDGRFRAYGQ
     DLLVRKGELL FSGPPDQPLL NIEAIRNPDA TEDDVIAGVR VTGTADEPKA EIFSDPAMPQ
     AEALSYLLRG QGLDSNQSDS AAMTSMLIGL GVAQSGQVVG KIGETFGVSN LALDTQGVGD
     SSQVVVSGYV LPGLQVKYGV GIFDSLATLT LRYRLMPKLY LEAVSGVDQA LDLLYQFEF