TAMO_DROME
ID TAMO_DROME Reviewed; 797 AA.
AC Q9W1A4; Q0E8W9; Q8SZS5;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Protein tamozhennic;
GN Name=tamo; ORFNames=CG4057;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH DL; MSL-1; RAN;
RP RAN-LIKE AND MBO, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo {ECO:0000269|PubMed:12653959}, and
RC Ovary {ECO:0000269|PubMed:12653959};
RX PubMed=12653959; DOI=10.1046/j.1365-2443.2002.00634.x;
RA Minakhina S., Yang J., Steward R.;
RT "Tamo selectively modulates nuclear import in Drosophila.";
RL Genes Cells 8:299-310(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAN71241.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAN71241.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Has an essential role during oogenesis and embryogenesis,
CC perhaps in modulating the levels of nuclear import of additional
CC proteins. Modulates the nuclear import of dorsal (dl), Dif and male
CC specific lethal 1 (msl-1). Negatively regulates nuclear import of dl
CC and controls the accumulation of dl in the nucleus after immune
CC challenge. {ECO:0000269|PubMed:12653959}.
CC -!- SUBUNIT: Homomultimer. Binds to dl and msl-1 via their nuclear
CC localization signal (NLS). Also binds to Ran, Ran-like and mbo.
CC {ECO:0000269|PubMed:12653959}.
CC -!- INTERACTION:
CC Q9W1A4; P15330: dl; NbExp=4; IntAct=EBI-91385, EBI-198375;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12653959}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically, zygotic
CC expression is seen throughout development.
CC {ECO:0000269|PubMed:12653959}.
CC -!- MISCELLANEOUS: 'Tamozhennic' means 'customs officer' in Russian.
CC {ECO:0000303|PubMed:12653959}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL48016.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the N-terminal part.; Evidence={ECO:0000305};
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DR EMBL; AE013599; AAF47169.1; -; Genomic_DNA.
DR EMBL; AY070545; AAL48016.1; ALT_SEQ; mRNA.
DR EMBL; BT001486; AAN71241.1; -; mRNA.
DR RefSeq; NP_001286827.1; NM_001299898.1.
DR RefSeq; NP_611889.1; NM_138045.2.
DR RefSeq; NP_726426.1; NM_166664.1.
DR AlphaFoldDB; Q9W1A4; -.
DR SMR; Q9W1A4; -.
DR BioGRID; 63443; 30.
DR IntAct; Q9W1A4; 13.
DR STRING; 7227.FBpp0072188; -.
DR PaxDb; Q9W1A4; -.
DR DNASU; 37864; -.
DR EnsemblMetazoa; FBtr0072281; FBpp0072188; FBgn0041582.
DR EnsemblMetazoa; FBtr0072282; FBpp0072189; FBgn0041582.
DR EnsemblMetazoa; FBtr0343438; FBpp0310082; FBgn0041582.
DR GeneID; 37864; -.
DR KEGG; dme:Dmel_CG4057; -.
DR UCSC; CG4057-RA; d. melanogaster.
DR CTD; 37864; -.
DR FlyBase; FBgn0041582; tamo.
DR VEuPathDB; VectorBase:FBgn0041582; -.
DR eggNOG; ENOG502RR2T; Eukaryota.
DR GeneTree; ENSGT00530000063956; -.
DR HOGENOM; CLU_310844_0_0_1; -.
DR InParanoid; Q9W1A4; -.
DR OMA; VQHDRKF; -.
DR OrthoDB; 312617at2759; -.
DR PhylomeDB; Q9W1A4; -.
DR SignaLink; Q9W1A4; -.
DR BioGRID-ORCS; 37864; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 37864; -.
DR PRO; PR:Q9W1A4; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0041582; Expressed in seminal fluid secreting gland and 11 other tissues.
DR ExpressionAtlas; Q9W1A4; baseline and differential.
DR Genevisible; Q9W1A4; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0031267; F:small GTPase binding; IPI:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042306; P:regulation of protein import into nucleus; IMP:UniProtKB.
DR InterPro; IPR036339; PUB-like_dom_sf.
DR InterPro; IPR018997; PUB_domain.
DR InterPro; IPR029746; Tamozhennic.
DR InterPro; IPR001876; Znf_RanBP2.
DR InterPro; IPR036443; Znf_RanBP2_sf.
DR PANTHER; PTHR15326:SF2; PTHR15326:SF2; 1.
DR Pfam; PF09409; PUB; 1.
DR SMART; SM00547; ZnF_RBZ; 1.
DR SUPFAM; SSF143503; SSF143503; 1.
DR SUPFAM; SSF90209; SSF90209; 1.
DR PROSITE; PS01358; ZF_RANBP2_1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Metal-binding; Protein transport;
KW Reference proteome; Transport; Zinc; Zinc-finger.
FT CHAIN 1..797
FT /note="Protein tamozhennic"
FT /id="PRO_0000072425"
FT DOMAIN 79..146
FT /note="PUB"
FT /evidence="ECO:0000255"
FT ZN_FING 735..766
FT /note="RanBP2-type"
FT /evidence="ECO:0000255"
FT REGION 515..570
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 638..697
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 523..557
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 655..676
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 797 AA; 87914 MW; 3362970101C170DE CRC64;
MSDFVPHDLI PDLWDEILRR HWMFLETEES IEKLEERKQL EGCLKEFLCV VQHDRKFFLP
ETGHVLRRSV LELPDFSAQN AIVAFETISQ YANNLFTKPW RKEYRTLKTY SGCFQHDIQS
RLLDAEQLFL AMGYRRAAED TFVLEGPICP DQVTNVSRDA MAAYVECQIM KHIYAGVTAA
GYVCTWKDIL QFRERYVGGT STTIKEMVRQ LSEKRVRMEQ PPMQENTYSN VVSAAPTACG
MRSNNVAHHP SSSGTCALHP NGLNEAGKYL PPYPAPPPQQ PHLPGPLMTH SRSLDHYQEP
QAHLPHRHSF DQQLQQQCQL PHVYEAPYDC LDGLSMGSSA SYAAVTGAYN APGNRYPLPY
NISSQLNAPY ASPADFYGNG QHTNMYATLG KTGPAHSCDL HRRQPNTSAA AAAALAAMQH
RQSTYPPDHH LIDFDERAHL TQHDFGTHDY DPQYAELRGQ VQPSMRGNPV AMYATYGGYD
LPTTLPQAPP PTGQDMYIYA RPVPKSSRMR ALAEAGGINS TDKHPRSAEK QSTMDNNRKM
HKELKERNSR TAPAKRSGTE RDIPTTISDL NSYDSASLDG FVALDSSSSP PLMPKVQEGV
GSFESWNYVF KNLERSGYTK DLGDREDLLV QSLDLGSLSI TNGGAAPPAE KRREATNPTN
GEKARTLDKK SGTGRREAKV VQAPAPSPLP NSSSAGVKKV KSALKTAVVD NRGTGSRQRT
GAVPKQPPNV SPQLIVTSPN EWSCSFCTFL NPDTKRICEM CCRSKDFNLE AASAASSSAA
AAAASAASVS HASSTCV
