TAM_AFIC5
ID TAM_AFIC5 Reviewed; 256 AA.
AC B6JIA0; F8BSX6;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=Trans-aconitate 2-methyltransferase {ECO:0000255|HAMAP-Rule:MF_00560};
DE EC=2.1.1.144 {ECO:0000255|HAMAP-Rule:MF_00560};
GN Name=tam {ECO:0000255|HAMAP-Rule:MF_00560};
GN OrderedLocusNames=OCAR_7047, OCA5_c10480;
OS Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)
OS (Oligotropha carboxidovorans).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Afipia.
OX NCBI_TaxID=504832;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49405 / DSM 1227 / KCTC 32145 / OM5;
RX PubMed=18539730; DOI=10.1128/jb.00614-08;
RA Paul D., Bridges S., Burgess S.C., Dandass Y., Lawrence M.L.;
RT "Genome sequence of the chemolithoautotrophic bacterium Oligotropha
RT carboxidovorans OM5T.";
RL J. Bacteriol. 190:5531-5532(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49405 / DSM 1227 / KCTC 32145 / OM5;
RX PubMed=21742883; DOI=10.1128/jb.05619-11;
RA Volland S., Rachinger M., Strittmatter A., Daniel R., Gottschalk G.,
RA Meyer O.;
RT "Complete genome sequences of the chemolithoautotrophic Oligotropha
RT carboxidovorans strains OM4 and OM5.";
RL J. Bacteriol. 193:5043-5043(2011).
CC -!- FUNCTION: Catalyzes the S-adenosylmethionine monomethyl esterification
CC of trans-aconitate. {ECO:0000255|HAMAP-Rule:MF_00560}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + trans-aconitate = (E)-3-
CC (methoxycarbonyl)pent-2-enedioate + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:14969, ChEBI:CHEBI:15708, ChEBI:CHEBI:57470,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.144;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00560};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00560}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. Tam family.
CC {ECO:0000255|HAMAP-Rule:MF_00560}.
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DR EMBL; CP001196; ACI94155.1; -; Genomic_DNA.
DR EMBL; CP002826; AEI05767.1; -; Genomic_DNA.
DR RefSeq; WP_012564181.1; NC_015684.1.
DR AlphaFoldDB; B6JIA0; -.
DR SMR; B6JIA0; -.
DR STRING; 504832.OCAR_7047; -.
DR EnsemblBacteria; AEI05767; AEI05767; OCA5_c10480.
DR KEGG; oca:OCAR_7047; -.
DR KEGG; ocg:OCA5_c10480; -.
DR PATRIC; fig|504832.7.peg.1114; -.
DR eggNOG; COG4106; Bacteria.
DR HOGENOM; CLU_037990_5_2_5; -.
DR OMA; RFDARYY; -.
DR OrthoDB; 1739932at2; -.
DR Proteomes; UP000007730; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030798; F:trans-aconitate 2-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.290; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_00560; Tran_acon_Me_trans; 1.
DR InterPro; IPR041698; Methyltransf_25.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR023506; Trans-aconitate_MeTrfase.
DR InterPro; IPR023149; Trans_acon_MeTrfase_C.
DR Pfam; PF13649; Methyltransf_25; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..256
FT /note="Trans-aconitate 2-methyltransferase"
FT /id="PRO_1000129263"
SQ SEQUENCE 256 AA; 29010 MW; 1DD87A11363E7CA8 CRC64;
MADWSAEQYL KFEDERTRPA RDLLAQIPRL EARQIADIGC GPGNSTELLA RRWPQAKIIG
IDTSADMLRQ ARERLPDATF IEANVAHWVP PAGTDILFAN AIFQWVPDHL TQFKRLAEGL
PEGGVLAVQM PDNLDQPSHA LMRQVAQLPQ FRKQLAHAAE ARAALPHPSV YYDALRPLGR
ALDIWHTVYH HALDDAAAIV EWVKGTGLRP FLDPLDFPER KEFLEAYTAR IAEAYPPRID
GKVLLRFPRF FIVLTR
