ID   TAM_PSEA7               Reviewed;         275 AA.
AC   A6V4P3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Trans-aconitate 2-methyltransferase {ECO:0000255|HAMAP-Rule:MF_00560};
DE            EC=2.1.1.144 {ECO:0000255|HAMAP-Rule:MF_00560};
GN   Name=tam {ECO:0000255|HAMAP-Rule:MF_00560}; OrderedLocusNames=PSPA7_2668;
OS   Pseudomonas aeruginosa (strain PA7).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=381754;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PA7;
RA   Dodson R.J., Harkins D., Paulsen I.T.;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the S-adenosylmethionine monomethyl esterification
CC       of trans-aconitate. {ECO:0000255|HAMAP-Rule:MF_00560}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + trans-aconitate = (E)-3-
CC         (methoxycarbonyl)pent-2-enedioate + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:14969, ChEBI:CHEBI:15708, ChEBI:CHEBI:57470,
CC         ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.144;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00560};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00560}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. Tam family.
CC       {ECO:0000255|HAMAP-Rule:MF_00560}.
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DR   EMBL; CP000744; ABR84604.1; -; Genomic_DNA.
DR   RefSeq; WP_012075509.1; NC_009656.1.
DR   AlphaFoldDB; A6V4P3; -.
DR   SMR; A6V4P3; -.
DR   PRIDE; A6V4P3; -.
DR   EnsemblBacteria; ABR84604; ABR84604; PSPA7_2668.
DR   KEGG; pap:PSPA7_2668; -.
DR   HOGENOM; CLU_037990_5_2_6; -.
DR   OMA; RFDARYY; -.
DR   OrthoDB; 1739932at2; -.
DR   Proteomes; UP000001582; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030798; F:trans-aconitate 2-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.290; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   HAMAP; MF_00560; Tran_acon_Me_trans; 1.
DR   InterPro; IPR041698; Methyltransf_25.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR023506; Trans-aconitate_MeTrfase.
DR   InterPro; IPR023149; Trans_acon_MeTrfase_C.
DR   Pfam; PF13649; Methyltransf_25; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..275
FT                   /note="Trans-aconitate 2-methyltransferase"
FT                   /id="PRO_1000056571"
SQ   SEQUENCE   275 AA;  31702 MW;  893D207AE41665AD CRC64;
     MVRDDLRCRA GNETHWDPTA YMQFARLRQR PVVELLDHVD LYSPERIYDL GCGTGIATEL
     LARRWPQAEL HGVDSSAEML EEAARLPIRA SWERANLQHW CAERPASLLF AAAVLHFIER
     HCSLLPRLLG QLSPGGCLAA HMPNWRDASW YRLMLDALDS AGPGGTPLGS PTLRYLMQQR
     NVLSLDNYYR LLAPLCAEVD IWETEHLQVV DGNDPIFDWV KVSALRPVLG ELDEEARRRF
     LDRYLDLLHR YYPQELDGRT LFPFRRVFVV ASLRR