TAM_PSEAE
ID TAM_PSEAE Reviewed; 275 AA.
AC Q9I0S1;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Trans-aconitate 2-methyltransferase {ECO:0000255|HAMAP-Rule:MF_00560};
DE EC=2.1.1.144 {ECO:0000255|HAMAP-Rule:MF_00560};
GN Name=tam {ECO:0000255|HAMAP-Rule:MF_00560}; OrderedLocusNames=PA2564;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Catalyzes the S-adenosylmethionine monomethyl esterification
CC of trans-aconitate. {ECO:0000255|HAMAP-Rule:MF_00560}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + trans-aconitate = (E)-3-
CC (methoxycarbonyl)pent-2-enedioate + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:14969, ChEBI:CHEBI:15708, ChEBI:CHEBI:57470,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.144;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00560};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00560}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. Tam family.
CC {ECO:0000255|HAMAP-Rule:MF_00560}.
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DR EMBL; AE004091; AAG05952.1; -; Genomic_DNA.
DR PIR; H83325; H83325.
DR RefSeq; NP_251254.1; NC_002516.2.
DR RefSeq; WP_003113340.1; NZ_QZGE01000008.1.
DR AlphaFoldDB; Q9I0S1; -.
DR SMR; Q9I0S1; -.
DR STRING; 208964.PA2564; -.
DR PaxDb; Q9I0S1; -.
DR PRIDE; Q9I0S1; -.
DR DNASU; 882722; -.
DR EnsemblBacteria; AAG05952; AAG05952; PA2564.
DR GeneID; 882722; -.
DR KEGG; pae:PA2564; -.
DR PATRIC; fig|208964.12.peg.2684; -.
DR PseudoCAP; PA2564; -.
DR HOGENOM; CLU_037990_5_2_6; -.
DR InParanoid; Q9I0S1; -.
DR OMA; RFDARYY; -.
DR PhylomeDB; Q9I0S1; -.
DR BioCyc; PAER208964:G1FZ6-2600-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030798; F:trans-aconitate 2-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.290; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_00560; Tran_acon_Me_trans; 1.
DR InterPro; IPR041698; Methyltransf_25.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR023506; Trans-aconitate_MeTrfase.
DR InterPro; IPR023149; Trans_acon_MeTrfase_C.
DR Pfam; PF13649; Methyltransf_25; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..275
FT /note="Trans-aconitate 2-methyltransferase"
FT /id="PRO_0000218084"
SQ SEQUENCE 275 AA; 31674 MW; A902E2B71FF2E06B CRC64;
MVHDLLRDRA DLEARWNPSA YMQFARLRQR PVVELLDHIE PCCPERIYDL GCGTGIATEL
LARRWPLAEL YGVDSSVEML EEAARLPIKA SWERANLRHW CAERPASLLF AAAVLHFLED
HGKLLPRLLG QLTPGGCLAA HMPNWRDASW YRLMLDALDS AGPGGASLGS PALRYRLHRR
NVLSLDNYYR LLAPLTAELD IWETEYLQVV DGNDPIFDWI KVSALRPVLG ELDEEARRRF
LDRYLELLHR HYPRELDGRT LFPFRRVFIV ASLGR
