ID   TANA_ARATH              Reviewed;         473 AA.
AC   Q197W8;
DT   18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Microtubule-binding protein TANGLED;
DE            Short=AtTAN;
GN   Name=TAN; Synonyms=ATN;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION, TISSUE
RP   SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=17964159; DOI=10.1016/j.cub.2007.09.063;
RA   Walker K.L., Muller S., Moss D., Ehrhardt D.W., Smith L.G.;
RT   "Arabidopsis TANGLED identifies the division plane throughout mitosis and
RT   cytokinesis.";
RL   Curr. Biol. 17:1827-1836(2007).
RN   [2]
RP   INTERACTION WITH POK1.
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=16682350; DOI=10.1016/j.cub.2006.03.034;
RA   Muller S., Han S., Smith L.G.;
RT   "Two kinesins are involved in the spatial control of cytokinesis in
RT   Arabidopsis thaliana.";
RL   Curr. Biol. 16:888-894(2006).
RN   [3]
RP   SUBCELLULAR LOCATION, AND INTERACTION WITH POK1.
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=21172800; DOI=10.1242/jcs.073676;
RA   Rasmussen C.G., Sun B., Smith L.G.;
RT   "Tangled localization at the cortical division site of plant cells occurs
RT   by several mechanisms.";
RL   J. Cell Sci. 124:270-279(2011).
CC   -!- FUNCTION: Is required for spatial control cell division during plant
CC       development. Through an association with microtubules, acts both for
CC       the positioning of cytoskeletal arrays that establish planes of cell
CC       division during prophase and for spatial guidance of expanding
CC       phragmoplasts toward preestablished cortical division sites (CDS)
CC       during cytokinesis. {ECO:0000269|PubMed:17964159}.
CC   -!- SUBUNIT: Interacts with POK1. {ECO:0000269|PubMed:16682350,
CC       ECO:0000269|PubMed:21172800}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:21172800}.
CC       Nucleus {ECO:0000269|PubMed:21172800}. Cytoplasm, cytoskeleton,
CC       phragmoplast {ECO:0000269|PubMed:17964159,
CC       ECO:0000269|PubMed:21172800}. Note=Preferentially localized to the
CC       preprophase band (PPB) during early stage of mitotis and later
CC       localized to the cortical division sites (CDS) during cytokinesis.
CC       {ECO:0000269|PubMed:17964159}.
CC   -!- TISSUE SPECIFICITY: Strongly expressed in flower buds and root tips.
CC       {ECO:0000269|PubMed:17964159}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC       {ECO:0000269|PubMed:17964159}.
CC   -!- CAUTION: In cv. Columbia (AC Q84M91), a naturally occurring frameshift
CC       at position 444 results in a shortened C-terminus. The sequence shown
CC       is from strain cv. Landsberg erecta. {ECO:0000305}.
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DR   EMBL; DQ631804; ABF84064.1; -; mRNA.
DR   AlphaFoldDB; Q197W8; -.
DR   iPTMnet; Q197W8; -.
DR   PRIDE; Q197W8; -.
DR   EnsemblPlants; AT3G05330.1; AT3G05330.1; AT3G05330.
DR   Gramene; AT3G05330.1; AT3G05330.1; AT3G05330.
DR   PRO; PR:Q197W8; -.
DR   ExpressionAtlas; Q197W8; baseline and differential.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0009524; C:phragmoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR   GO; GO:2000694; P:regulation of phragmoplast microtubule organization; IEA:InterPro.
DR   InterPro; IPR044709; TAN1.
DR   PANTHER; PTHR35728; PTHR35728; 2.
PE   1: Evidence at protein level;
KW   Cytoplasm; Cytoskeleton; Nucleus.
FT   CHAIN           1..473
FT                   /note="Microtubule-binding protein TANGLED"
FT                   /id="PRO_0000423585"
FT   REGION          1..132
FT                   /note="Required for binding to TAN and location to the
FT                   cortical division sites (CDS) during cytokinesis"
FT   REGION          131..218
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          290..354
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        131..156
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        171..186
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        187..218
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        305..354
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   473 AA;  52873 MW;  5D5F003F9B2196C4 CRC64;
     MVARTPQKQR KVAMVVPPLN SDLLKETINK VDKCMERLQE LQYTIAGGTK VVSGVNLSPR
     STRIYLKTSL RCKQETLRIK NATNKKSPVG KFPASSPGDW RKMSLPAMLL GETVNEILQA
     SQVTRDIVDA IAPKKSRKSR RLTMSQEDDG PKTPETQQKS REQNPETVSS NIKARRKKEK
     QNRRSESDSP PSLQRARSRI AFRTISPQVK GNNGENSFRH LANRVSPKHK PWVKKAVLFP
     NPLFISGTAT QQAKFSRTMS PVIARNEISS IKNNKETPYK FLIKSPPTSA SKFQVKIRSP
     PKVLVSPTRN GSNSVRKSPR GSRSPTRTVN LGKKSASISP IRNTGKRSPK LSTAAKLRRS
     FTPTRNGSNL ARKSSISPKR VTLQAFLSPT RNGNFCKKSP KASISPTRVC NKSQKLSTAA
     KFRRSFSPSR LAMRFVSPMK SRKSVAKCDD HEMVSGLKQR PVLVPKRFSI RRI