ID   TAN_BACIU               Reviewed;          15 AA.
AC   C0HJL9;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2014, sequence version 1.
DT   11-DEC-2019, entry version 6.
DE   RecName: Full=Tannase {ECO:0000303|Ref.2};
DE            EC=3.1.1.20 {ECO:0000269|Ref.2};
DE   AltName: Full=Tannin acylhydrolase {ECO:0000303|Ref.1};
DE   Flags: Fragment;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE.
RC   STRAIN=PAB2 {ECO:0000269|Ref.1};
RA   Jana A., Mondal K.C., Das Mohapatra P.K.;
RT   "Sequence insights of Tannase of Bacillus subtilis PAB2.";
RL   Submitted (JUN-2014) to UniProtKB.
RN   [2] {ECO:0000305}
RP   FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND SUBUNIT.
RC   STRAIN=PAB2 {ECO:0000269|Ref.2};
RX   DOI=10.1016/j.bej.2013.06.002;
RA   Jana A., Maity C., Halder S.K., Das A., Pati B.R., Mondal K.C.,
RA   Das Mohapatra P.K.;
RT   "Structural characterization of thermostable, solvent tolerant, cytosafe
RT   tannase from Bacillus subtilis PAB2.";
RL   Biochem. Eng. J. 77:161-170(2013).
CC   -!- FUNCTION: Hydrolyzes ester bonds of tannic acid to produce gallic acid
CC       and glucose. Exhibits no cytotoxicity in vitro in Vero cells or in vivo
CC       in a rat model. {ECO:0000269|Ref.2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=digallate + H2O = 2 3,4,5-trihydroxybenzoate + H(+);
CC         Xref=Rhea:RHEA:16365, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16918, ChEBI:CHEBI:17866; EC=3.1.1.20;
CC         Evidence={ECO:0000269|Ref.2};
CC   -!- ACTIVITY REGULATION: Activity is enhanced by the addition of 1 mM
CC       Mg(2+) (more than 100%) and by the addition of 1% glycerol (22%).
CC       Activity is reduced by the addition of 1 mM of various metal ions,
CC       including Hg(2+) (98%), Fe(3+) (95%), Fe(2+) (94%), Ba(2+) (92%),
CC       Ca(2+) (76%), Co(2+) (70%), Zn(2+) (44%), Ag(2+) (14%) and K(+) (8%).
CC       Inhibited by the addition of 1 mM DMSO (75%), Triton X-100 (42%),
CC       sodium azide (40%), beta-mercaptoethanol (34%), Tween 80 (22%), Tween
CC       60 (20%), EDTA (18%) and phenylmethanesulfonyl fluoride (PMSF) (7%) but
CC       not affected by the addition of SDS. Also inhibited by the addition of
CC       1% of acetic acid (73%), acetone (67%) and butanol (40%) but not
CC       affected by the addition of isopropanol, methanol and ethanol.
CC       {ECO:0000269|Ref.2}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.445 mM for tannic acid at 40 degrees Celsius and pH 5.0
CC         {ECO:0000269|Ref.2};
CC         Vmax=125.8 mM/min/mg enzyme with tannic acid as substrate
CC         {ECO:0000269|Ref.2};
CC         Note=Exhibits salt tolerance as it retains 67% of activity after 24 h
CC         exposure to 4 M NaCl. {ECO:0000269|Ref.2};
CC       pH dependence:
CC         Optimum pH is 5.0. Active from pH 3.0 to 7.0. {ECO:0000269|Ref.2};
CC       Temperature dependence:
CC         Optimum temperature is 40 degrees Celsius. Retains 14% of its maximal
CC         activity after heating 1 hour at 70 degrees Celsius.
CC         {ECO:0000269|Ref.2};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|Ref.2}.
CC   -!- SIMILARITY: Belongs to the tannase family.
CC       {ECO:0000250|UniProtKB:P78581}.
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DR   GO; GO:0050318; F:tannase activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Serine esterase.
FT   PEPTIDE         1..15
FT                   /note="Tannase"
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000430487"
FT   NON_TER         15
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   15 AA;  1798 MW;  319DF4660C905D26 CRC64;
     VQPPHSHGDN FYIWT