ID   TAO3_YEAST              Reviewed;        2376 AA.
AC   P40468; D6VVF8; Q45T93;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 170.
DE   RecName: Full=Cell morphogenesis protein PAG1;
DE   AltName: Full=Protein TAO3;
GN   Name=TAO3; Synonyms=PAG1; OrderedLocusNames=YIL129C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 200060 / W303, ATCC 204508 / S288c,
RC   ATCC 204626 / S288c / A364A, and Sigma 1278B;
RX   PubMed=16273108; DOI=10.1038/ng1674;
RA   Deutschbauer A.M., Davis R.W.;
RT   "Quantitative trait loci mapped to single-nucleotide resolution in yeast.";
RL   Nat. Genet. 37:1333-1340(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169870;
RA   Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA   Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA   Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA   Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA   Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL   Nature 387:84-87(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   FUNCTION, AND INTERACTION WITH CBK1.
RX   PubMed=11854408; DOI=10.1091/mbc.01-07-0365;
RA   Du L.L., Novick P.;
RT   "Pag1p, a novel protein associated with protein kinase Cbk1p, is required
RT   for cell morphogenesis and proliferation in Saccharomyces cerevisiae.";
RL   Mol. Biol. Cell 13:503-514(2002).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-2264 AND SER-2267, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2355, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA   Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA   Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT   "Analysis of phosphorylation sites on proteins from Saccharomyces
RT   cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141 AND THR-2264, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1144 AND THR-2264, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Seems to play a role in cell morphogenesis.
CC       {ECO:0000269|PubMed:11854408}.
CC   -!- SUBUNIT: Associates with CBK1.
CC   -!- INTERACTION:
CC       P40468; P53894: CBK1; NbExp=8; IntAct=EBI-18961, EBI-4110;
CC   -!- MISCELLANEOUS: Present with 206 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: To S.pombe mor2. {ECO:0000305}.
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DR   EMBL; DQ116812; AAZ23264.1; -; Genomic_DNA.
DR   EMBL; DQ116814; AAZ23266.1; -; Genomic_DNA.
DR   EMBL; DQ116817; AAZ23269.1; -; Genomic_DNA.
DR   EMBL; DQ116824; AAZ23276.1; -; Genomic_DNA.
DR   EMBL; Z38059; CAA86149.1; -; Genomic_DNA.
DR   EMBL; BK006942; DAA08424.1; -; Genomic_DNA.
DR   PIR; S48405; S48405.
DR   RefSeq; NP_012137.3; NM_001179477.3.
DR   AlphaFoldDB; P40468; -.
DR   BioGRID; 34862; 670.
DR   DIP; DIP-5950N; -.
DR   IntAct; P40468; 12.
DR   MINT; P40468; -.
DR   STRING; 4932.YIL129C; -.
DR   iPTMnet; P40468; -.
DR   MaxQB; P40468; -.
DR   PaxDb; P40468; -.
DR   PRIDE; P40468; -.
DR   EnsemblFungi; YIL129C_mRNA; YIL129C; YIL129C.
DR   GeneID; 854677; -.
DR   KEGG; sce:YIL129C; -.
DR   SGD; S000001391; TAO3.
DR   VEuPathDB; FungiDB:YIL129C; -.
DR   eggNOG; KOG1825; Eukaryota.
DR   GeneTree; ENSGT00610000086058; -.
DR   HOGENOM; CLU_000325_0_0_1; -.
DR   InParanoid; P40468; -.
DR   OMA; GPTQFCQ; -.
DR   BioCyc; YEAST:G3O-31380-MON; -.
DR   PRO; PR:P40468; -.
DR   Proteomes; UP000002311; Chromosome IX.
DR   RNAct; P40468; protein.
DR   GO; GO:0005938; C:cell cortex; IBA:GO_Central.
DR   GO; GO:0005933; C:cellular bud; IDA:SGD.
DR   GO; GO:0000131; C:incipient cellular bud site; IDA:SGD.
DR   GO; GO:0043332; C:mating projection tip; IDA:SGD.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0030427; C:site of polarized growth; IBA:GO_Central.
DR   GO; GO:0007118; P:budding cell apical bud growth; IMP:SGD.
DR   GO; GO:0007114; P:cell budding; IMP:SGD.
DR   GO; GO:0000902; P:cell morphogenesis; IMP:SGD.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR025614; Cell_morpho_N.
DR   InterPro; IPR025481; Cell_Morphogen_C.
DR   InterPro; IPR039867; Furry/Tao3/Mor2.
DR   InterPro; IPR029473; MOR2-PAG1_mid.
DR   PANTHER; PTHR12295; PTHR12295; 1.
DR   Pfam; PF14225; MOR2-PAG1_C; 1.
DR   Pfam; PF14228; MOR2-PAG1_mid; 3.
DR   Pfam; PF14222; MOR2-PAG1_N; 1.
DR   SUPFAM; SSF48371; SSF48371; 2.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..2376
FT                   /note="Cell morphogenesis protein PAG1"
FT                   /id="PRO_0000072430"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          275..294
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         141
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         1144
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MOD_RES         2264
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
FT   MOD_RES         2267
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950"
FT   MOD_RES         2355
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17287358"
SQ   SEQUENCE   2376 AA;  269858 MW;  08958191BE969C1B CRC64;
     MASRFTFPPQ RDQGIGFTFP PTNKAEGSSN NNQISIDIDP SGQDVLEEIN EAPLNTFPLH
     QSVTDAPIID IPSPTDMSEG TSLNNQLLLR QQQQQGTGEG QALPPTFVEE QSDQNKISML
     LPEQKQQRMQ ESAPPDITAK SVAEDYVTTL RQQMATDWKS PSEYALHILF TKFIRYAENK
     LNMCLQQLDM AEPPIVEILG EGVDPSFDEI IKSLGHIAKK KPKPVIDAMM FWRKTKSEAA
     NSASEEMEKL LKEYEFEKAH PSQAHFLMNR RLSRSSSNTT SKYKHNNNTN NLPGMKRHVS
     SSFNNKVPLI KASSSNNSAT SSPSIANSQL KSLENTIEVA KEEAFLADRK SLISIYILCR
     VLNEIVKQAS SNEEEDLSDK LEEIVFTQLK TTDPLSISTS LIKSSNWNSF AELLGSMSEK
     KFLSVSDRFI ADLEKIPAYI PPELEPSTHL LILGMRYLKL RNYPLEKFEE SADFMKSLSK
     FFAKTENFPV CLAYAEVTNQ LLLPLAGSLT AEVNHPTWVE AMSTLLNTAK RLQADSKYWV
     SGFKLTVSIL CASPPDLFSK QWLSLLEANA SKVKSKSLNE RIIFAVGLSR LVWVYLYRCP
     ETLNNTTRTL TKLLQLYLNT RKKENWITGD FGLLNPLTDA LISIGFLHPN FLMEQALIPL
     IRQSFNGSNL ENINYEKLIL TINTYKGLLV TKERPRFPED DNRLYELNLN NITVNQVQEA
     SSINHTEISD YFYKLFLLLD SSIGSEVWSP ENQHQKQSSN AFSPFGFSFS NDNDSSKNKS
     LYVILFGTII EAIPCCLSIS RTIPYKSTIE ILSRNAVHSE VIISSSSQNA LRALASKKNP
     YTLITWFAKY SFDFDEKTQS SYNMSYLSSK EYNRLLILYV ELLECWLEEF QSSNKEENKK
     ETGLDGIRLL PIDAEQEESN ETEKLEWKNT VTVIEEVEGN GLFFLCSHDA KIRRLGIQIL
     RIIFKFDEAM MEKTEKLSNG HSRSSSHFAA DRGTRLIDLL NECNTTTLIN PHKATLSAVE
     KTRFSRLNSK YKRGLLIKLA ESEYGVDAAL WQRAFPKLLA LVFKTCPMAM ALCRSIVCIR
     LVQVHEIILR VANDVDFKLK NVLPETIVNQ WKLYLIAACT SLTSTFDQKL HIPSNIPQHG
     RKKSQQIFTV QHQKIKSAKS IFKMVLPLLN AKYIMIRDAI ITGLSSMNIN IFKAYVEAID
     VFLVAWKEGS SNNQIRVEMF HILTILSPYL KSDMIFNDEW ILRKLSEFLQ KTKQFLEKDS
     VQISYEYQSL RSYFAGLILS YYMAVREHPL IDELFPFQAR ASCFNFLKEW CGYGEYEPIS
     EERYAIMIKN TESGRDRTAI TTGIEFQKNR LQMIVLETMV VLCSDPITQT LDDDLELPIV
     ISFDTEDLLA WIEALFDSDN TTVKNLGVRA LENLLDKNRE NFKLFRDVAF QCVSHHSHPS
     VAVLYYTTLC KSVLKLDNLV LDEDELVSLG LYGLVADKED TRTFAVDLLS AVETKLHNSS
     YTKVFKERLA NSSKTVYKST AKEISSIFAE LLSQDLCLRI FSSLVRILDL FPFEIKRDLL
     VLMVPWVNKF TLKSLEELDT FMVLNNLFYI TIDLNDSLPN EVEQLWISLG KGNSFQNIHV
     SLEYIINSSM NHCNPLFVQY ARDIVLYLAN IPGGIGLLDT LLNNLEPKYM VPLAKHTFNE
     PMNNNKYSFL GNIWERLNYN GKRIIFSKAQ LSIIFLVNLL TNLSESVKAK IPLLLHMSIC
     LLDHYVPLIH ESACKIASTL IFGLAPSHEK SEETVKLLRN KHALWSYDNL MKKGARSPKT
     MDLLIRNIIS IFSDLDEFQV TWQRIALKWA TTCSVRHIAC RSFQIFRSLL TFLDQEMLRD
     MLHRLSNTIS DGNVDIQGFA MQILMTLNAI MAELDPTNLI SFPQLFWSIT ACLSSIHEQE
     FIEVLSCLSK FISKIDLDSP DTVQCLVAIF PSNWEGRFDG LQQIVMTGLR SANSLEITWK
     FLDKLNLLKD SRIIANTESR LLFALIANLP RFLNAMDRKD FTGIQVAADS LIELANAYKQ
     PSLSRLIDSL AKNKFRSKKD FMSQVVSFIS RNYFPSYSAQ TLVFLLGLLF NKIGWIRVQT
     LEILKYVFPL IDLRRPEFIG VGADLISPLL RLLFTEYEAK ALEVLDCVPN VSGSKMDKDV
     LRITMGNKDV KDGDNATTTL FGLPEDSGWS VPMPTMTAAT TRHNVHAVFM TCGTGKSDEV
     SAHGSDDMDA VIEFHADGDY ELGRMDTIVE FHADGDYDLG RMDTNDSISV AEEKDASLSH
     MWAELDNLDS FFTKDTNVPN ISSKMGMGIP HGRSDSIETT RTDQTFSFES APQLYDKKVS
     VILNRSLSRT PSNVSFKTHL ADSFAVKINR NGKPRI