ID   TAP25_BPML5             Reviewed;         272 AA.
AC   Q05232; W1JB50;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   11-NOV-2015, sequence version 2.
DT   02-JUN-2021, entry version 58.
DE   RecName: Full=Tail assembly protein Gp25 {ECO:0000303|PubMed:8459766};
DE   AltName: Full=Gene 25 protein;
DE   AltName: Full=Gp25;
GN   Name=25; ORFNames=PBI_L5_25 {ECO:0000312|EMBL:CDL92968.1};
OS   Mycobacterium phage L5 (Mycobacteriophage L5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Fromanvirus.
OX   NCBI_TaxID=31757;
OH   NCBI_TaxID=1763; Mycobacterium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8459766; DOI=10.1111/j.1365-2958.1993.tb01131.x;
RA   Hatfull G.F., Sarkis G.J.;
RT   "DNA sequence, structure and gene expression of mycobacteriophage L5: a
RT   phage system for mycobacterial genetics.";
RL   Mol. Microbiol. 7:395-405(1993).
RN   [2]
RP   RIBOSOMAL FRAMESHIFT.
RX   PubMed=15469818; DOI=10.1016/j.molcel.2004.09.006;
RA   Xu J., Hendrix R.W., Duda R.L.;
RT   "Conserved translational frameshift in dsDNA bacteriophage tail assembly
RT   genes.";
RL   Mol. Cell 16:11-21(2004).
CC   -!- FUNCTION: Promotes tail assembly by creating a scaffold for the tail
CC       tube proteins. The tail assembly proteins Gp24 and Gp25 would wrap the
CC       linear tape measure protein to create a tail assembly scaffold. It
CC       would allow polymerization of tail tube protein during which Gp24 and
CC       Gp25 are released and therefore are absent from the mature virion. The
CC       tail assembly protein Gp25 is produced by a rare -1 ribosomal
CC       frameshift. The ratio Gp24/Gp25 is important for proper tail assembly.
CC       {ECO:0000250|UniProtKB:P03734}.
CC   -!- SUBUNIT: Interacts with tail assembly protein Gp24 and tape measure
CC       protein. {ECO:0000250|UniProtKB:P03734}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC         Comment=A readthrough event might also occur in addition to the
CC         frameshifting event. {ECO:0000269|PubMed:15469818};
CC       Name=Tail assembly protein Gp25 {ECO:0000269|PubMed:15469818};
CC         IsoId=Q05232-1; Sequence=Displayed;
CC       Name=Tail assembly protein Gp24 {ECO:0000269|PubMed:15469818};
CC         IsoId=Q05231-1; Sequence=External;
CC   -!- MISCELLANEOUS: [Isoform Tail assembly protein Gp25]: Produced by -1
CC       ribosomal frameshifting.
CC   -!- SIMILARITY: Belongs to the L5likevirus tail assembly protein family.
CC       {ECO:0000305}.
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DR   EMBL; Z18946; CDL92968.1; -; Genomic_DNA.
DR   PIR; S30970; S30970.
DR   RefSeq; YP_009000607.1; NC_001335.1. [Q05232-1]
DR   GeneID; 18261516; -.
DR   KEGG; vg:18261516; -.
DR   Proteomes; UP000002123; Genome.
DR   GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-KW.
DR   InterPro; IPR020132; Gp24/Gp25.
DR   Pfam; PF17388; GP24_25; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Ribosomal frameshifting; Viral release from host cell;
KW   Viral tail assembly.
FT   CHAIN           1..272
FT                   /note="Tail assembly protein Gp25"
FT                   /id="PRO_0000164739"
FT   REGION          219..272
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        219..242
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        256..272
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   272 AA;  30881 MW;  0C8BEA9637CFDB10 CRC64;
     MTNVFTIDAF REEVKKKYAP VLIGLSDDVT VELKPLLKLG QKAREAVVEV FKEFADIPDL
     EEDDDDELVD EYSLQVCDII AKAFRLIATK PKKLIAALDE EPDPRIRAEL YAAVLNTWKR
     ETQLGGSRAL AELIDKFGGA ILADLLQYYR VDLRDLFRDE DPLSPRFVLS LVLCLPKDGA
     FYAERRGGQQ YRGWTEDRYA LADIYDAIQA GNHILLLANR DPKKPKPKAP KSYPRPDDLE
     KTTPKPGSFA AMVVRAKKAA RERREREEES AE