ID   TAPA_AERHY              Reviewed;         142 AA.
AC   P45791;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Type IV pilus subunit protein TapA {ECO:0000303|PubMed:10858220};
DE   Flags: Precursor;
GN   Name=tapA;
OS   Aeromonas hydrophila.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=644;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ah65;
RX   PubMed=8820654; DOI=10.1046/j.1365-2958.1996.431958.x;
RA   Pepe C.M., Eklund M.W., Strom M.S.;
RT   "Cloning of an Aeromonas hydrophila type IV pilus biogenesis gene cluster:
RT   complementation of pilus assembly functions and characterization of a type
RT   IV leader peptidase/N-methyltransferase required for extracellular protein
RT   secretion.";
RL   Mol. Microbiol. 19:857-869(1996).
RN   [2]
RP   FUNCTION.
RX   PubMed=9344785; DOI=10.1006/mpat.1997.0152;
RA   Barnett T.C., Kirov S.M., Strom M.S., Sanderson K.;
RT   "Aeromonas spp. possess at least two distinct type IV pilus families.";
RL   Microb. Pathog. 23:241-247(1997).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC   STRAIN=Ah65N;
RX   PubMed=10858220; DOI=10.1128/iai.68.7.4040-4048.2000;
RA   Kirov S.M., Barnett T.C., Pepe C.M., Strom M.S., Albert M.J.;
RT   "Investigation of the role of type IV Aeromonas pilus (Tap) in the
RT   pathogenesis of Aeromonas gastrointestinal infection.";
RL   Infect. Immun. 68:4040-4048(2000).
CC   -!- FUNCTION: Major component of the type IV (TAP) pilus (PubMed:10858220).
CC       Aeromonas hydrophila possesses two distinct families of type IV pili:
CC       the bundle-forming pilus (Bfp) and the type IV pilus (Tap)
CC       (PubMed:9344785). {ECO:0000269|PubMed:10858220,
CC       ECO:0000269|PubMed:9344785}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:10858220}; Single-
CC       pass membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Mutants have no effect on the bacterial adherence
CC       to host epithelial and intestinal cell lines.
CC       {ECO:0000269|PubMed:10858220}.
CC   -!- SIMILARITY: Belongs to the N-Me-Phe pilin family. {ECO:0000305}.
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DR   EMBL; U20255; AAC43995.1; -; Genomic_DNA.
DR   PIR; S70872; S70872.
DR   AlphaFoldDB; P45791; -.
DR   SMR; P45791; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR012902; N_methyl_site.
DR   InterPro; IPR045584; Pilin-like.
DR   Pfam; PF07963; N_methyl; 1.
DR   SUPFAM; SSF54523; SSF54523; 1.
DR   TIGRFAMs; TIGR02532; IV_pilin_GFxxxE; 1.
DR   PROSITE; PS00409; PROKAR_NTER_METHYL; 1.
PE   3: Inferred from homology;
KW   Fimbrium biogenesis; Membrane; Methylation; Transmembrane;
KW   Transmembrane helix.
FT   PROPEP          1..6
FT                   /note="Leader sequence"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01070,
FT                   ECO:0000305|PubMed:9344785"
FT                   /id="PRO_0000024284"
FT   CHAIN           7..142
FT                   /note="Type IV pilus subunit protein TapA"
FT                   /id="PRO_0000024285"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         7
FT                   /note="N-methylphenylalanine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01070"
SQ   SEQUENCE   142 AA;  14069 MW;  1BDB32500962AA88 CRC64;
     MKKQSGFTLI ELMIVVAIVA ILAAIALPAY QTYTKKAKFT EVVSATGTYK SALEVCFQTV
     GSLLNCTNGT NGVPPAAGAS GLVTSVAVSG NTASAATITA TGDATTFGAT SNVYIMTAAG
     SNGQIIWTAP ASGSTCAGAG FC