ID   TAP_BOVIN               Reviewed;          64 AA.
AC   P25068; O97532;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Tracheal antimicrobial peptide;
DE            Short=TAP;
DE   Flags: Precursor;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 27-59.
RC   TISSUE=Tracheal epithelium;
RX   PubMed=2023943; DOI=10.1073/pnas.88.9.3952;
RA   Diamond G., Zasloff M., Eck H., Brasseur M., Maloy W.L., Bevins C.L.;
RT   "Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian
RT   tracheal mucosa: peptide isolation and cloning of a cDNA.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:3952-3956(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Trachea;
RX   PubMed=8506305; DOI=10.1073/pnas.90.10.4596;
RA   Diamond G., Jones D.E., Bevins C.L.;
RT   "Airway epithelial cells are the site of expression of a mammalian
RT   antimicrobial peptide gene.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:4596-4600(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Ryan L.K., Rhodes J., Bhat M., Diamond G.;
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Has antibacterial activity in vitro against Escherichia coli,
CC       Staphylococcus aureus, Klebsiella pneumonia, and Pseudomonas
CC       aeruginosa. In addition, the peptide is active against Candida
CC       albicans, indicating a broad spectrum of activity.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Tracheal epithelium.
CC   -!- SIMILARITY: Belongs to the beta-defensin family. LAP/TAP subfamily.
CC       {ECO:0000305}.
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DR   EMBL; M63023; AAB61757.1; -; Genomic_DNA.
DR   EMBL; L13373; AAA72363.1; -; Genomic_DNA.
DR   EMBL; AF014106; AAD01521.1; -; mRNA.
DR   PIR; A47438; A47438.
DR   RefSeq; NP_777201.1; NM_174776.1.
DR   AlphaFoldDB; P25068; -.
DR   SMR; P25068; -.
DR   STRING; 9913.ENSBTAP00000056499; -.
DR   PaxDb; P25068; -.
DR   Ensembl; ENSBTAT00000063757; ENSBTAP00000056499; ENSBTAG00000048171.
DR   GeneID; 286837; -.
DR   KEGG; bta:286837; -.
DR   CTD; 39935; -.
DR   VEuPathDB; HostDB:ENSBTAG00000048171; -.
DR   eggNOG; ENOG502SYUI; Eukaryota.
DR   GeneTree; ENSGT00940000160995; -.
DR   HOGENOM; CLU_189296_4_1_1; -.
DR   InParanoid; P25068; -.
DR   OMA; IRSGAIC; -.
DR   OrthoDB; 1584343at2759; -.
DR   Reactome; R-BTA-1461957; Beta defensins.
DR   Reactome; R-BTA-1461973; Defensins.
DR   Proteomes; UP000009136; Chromosome 27.
DR   Bgee; ENSBTAG00000048171; Expressed in olfactory segment of nasal mucosa and 58 other tissues.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0031731; F:CCR6 chemokine receptor binding; IBA:GO_Central.
DR   GO; GO:0042056; F:chemoattractant activity; IBA:GO_Central.
DR   GO; GO:0060326; P:cell chemotaxis; IBA:GO_Central.
DR   GO; GO:0042742; P:defense response to bacterium; IBA:GO_Central.
DR   InterPro; IPR001855; Defensin_beta-typ.
DR   InterPro; IPR006080; Defensin_beta/alpha.
DR   Pfam; PF00711; Defensin_beta; 1.
DR   SMART; SM00048; DEFSN; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000269|PubMed:2023943"
FT   PEPTIDE         27..64
FT                   /note="Tracheal antimicrobial peptide"
FT                   /id="PRO_0000006966"
FT   DISULFID        31..60
FT                   /evidence="ECO:0000250"
FT   DISULFID        38..53
FT                   /evidence="ECO:0000250"
FT   DISULFID        43..61
FT                   /evidence="ECO:0000250"
FT   CONFLICT        18
FT                   /note="W -> S (in Ref. 3; AAD01521)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        46
FT                   /note="S -> N (in Ref. 3; AAD01521)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   64 AA;  6954 MW;  68617B95E02918E6 CRC64;
     MRLHHLLLAL LFLVLSAWSG FTQGVGNPVS CVRNKGICVP IRCPGSMKQI GTCVGRAVKC
     CRKK