TAP_MYCBP
ID TAP_MYCBP Reviewed; 419 AA.
AC A0A0H3M5L9;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 16-SEP-2015, sequence version 1.
DT 25-MAY-2022, entry version 28.
DE RecName: Full=Multidrug efflux pump Tap {ECO:0000305};
GN Name=tap {ECO:0000303|PubMed:22232275};
GN OrderedLocusNames=BCG_1316c {ECO:0000312|EMBL:CAL71303.1};
OS Mycobacterium bovis (strain BCG / Pasteur 1173P2).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=410289;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BCG / Pasteur 1173P2;
RX PubMed=17372194; DOI=10.1073/pnas.0700869104;
RA Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P.,
RA Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K.,
RA Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C.,
RA Barrell B.G., Parkhill J., Cole S.T.;
RT "Genome plasticity of BCG and impact on vaccine efficacy.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007).
RN [2]
RP FUNCTION, ACTIVITY REGULATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=BCG / Pasteur 1173P2;
RX PubMed=22232275; DOI=10.1128/aac.05946-11;
RA Ramon-Garcia S., Mick V., Dainese E., Martin C., Thompson C.J.,
RA De Rossi E., Manganelli R., Ainsa J.A.;
RT "Functional and genetic characterization of the tap efflux pump in
RT Mycobacterium bovis BCG.";
RL Antimicrob. Agents Chemother. 56:2074-2083(2012).
CC -!- FUNCTION: Efflux pump that contributes to intrinsic antibiotic
CC resistance. The pump uses the electrochemical gradient as a source of
CC energy. Confers resistance to various antibiotics, including
CC tetracycline, acriflavine, spectinomycin and p-aminosalicylate. May be
CC involved in export of toxic by-products that accumulate during
CC stationary phase when nutrients are limited.
CC {ECO:0000269|PubMed:22232275}.
CC -!- ACTIVITY REGULATION: Efflux activity is inhibited by carbonyl cyanide
CC m-chlorophenylhydrazone (CCCP). {ECO:0000269|PubMed:22232275}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Disruption mutant shows changes in morphology,
CC growth defects and a progressive loss of viability upon subcultivation
CC in liquid medium. Disruption leads to an extensive change in gene
CC expression patterns during stationary phase, with no changes during
CC exponential growth. It also alters susceptibility to many antibiotics.
CC {ECO:0000269|PubMed:22232275}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Drug:H(+)
CC antiporter-3 (DHA3) (TC 2.A.1.21) family. {ECO:0000305}.
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DR EMBL; AM408590; CAL71303.1; -; Genomic_DNA.
DR RefSeq; WP_003406359.1; NC_008769.1.
DR AlphaFoldDB; A0A0H3M5L9; -.
DR SMR; A0A0H3M5L9; -.
DR KEGG; mbb:BCG_1316c; -.
DR HOGENOM; CLU_034180_2_0_11; -.
DR OMA; MVVNIPM; -.
DR Proteomes; UP000001472; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..419
FT /note="Multidrug efflux pump Tap"
FT /id="PRO_0000447328"
FT TRANSMEM 7..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..397
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 419 AA; 43287 MW; 69EA46BBFF653037 CRC64;
MRNSNRGPAF LILFATLMAA AGDGVSIVAF PWLVLQREGS AGQASIVASA TMLPLLFATL
VAGTAVDYFG RRRVSMVADA LSGAAVAGVP LVAWGYGGDA VNVLVLAVLA ALAAAFGPAG
MTARDSMLPE AAARAGWSLD RINGAYEAIL NLAFIVGPAI GGLMIATVGG ITTMWITATA
FGLSILAIAA LQLEGAGKPH HTSRPQGLVS GIAEGLRFVW NLRVLRTLGM IDLTVTALYL
PMESVLFPKY FTDHQQPVQL GWALMAIAGG GLVGALGYAV LAIRVPRRVT MSTAVLTLGL
ASMVIAFLPP LPVIMVLCAV VGLVYGPIQP IYNYVIQTRA AQHLRGRVVG VMTSLAYAAG
PLGLLLAGPL TDAAGLHATF LALALPIVCT GLVAIRLPAL RELDLAPQAD IDRPVGSAQ
