ID   BPP_BRAEP               Reviewed;           8 AA.
AC   P0DV72;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Bradykinin-potentiating peptide Brachy {ECO:0000303|PubMed:26661890};
DE            Short=BPP-Brachy {ECO:0000303|PubMed:26661890};
DE            Short=BPP-BrachyNH2 {ECO:0000303|PubMed:26661890};
DE   AltName: Full=Proline-rich oligopeptide {ECO:0000303|PubMed:26661890};
DE            Short=PRO {ECO:0000303|PubMed:26661890};
DE            Short=Proline-rich peptide {ECO:0000305};
OS   Brachycephalus ephippium (Pumpkin toadlet) (Bufo ephippium).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Brachycephalidae; Brachycephalus.
OX   NCBI_TaxID=164302;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, 3D-STRUCTURE MODELING OF
RP   BPP-BRACHYNH2 IN COMPLEX WITH ACE, MASS SPECTROMETRY, PARTIAL AMIDATION AT
RP   PRO-8, AND SYNTHESIS OF THE AMIDATED PEPTIDE.
RC   TISSUE=Skin secretion;
RX   PubMed=26661890; DOI=10.1371/journal.pone.0145071;
RA   Arcanjo D.D., Vasconcelos A.G., Comerma-Steffensen S.G., Jesus J.R.,
RA   Silva L.P., Pires Junior O.R., Costa-Neto C.M., Oliveira E.B., Migliolo L.,
RA   Franco O.L., Restini C.B., Paulo M., Bendhack L.M., Bemquerer M.P.,
RA   Oliveira A.P., Simonsen U., Leite J.R.;
RT   "A novel vasoactive proline-rich oligopeptide from the skin secretion of
RT   the frog Brachycephalus ephippium.";
RL   PLoS ONE 10:e0145071-e0145071(2015).
CC   -!- FUNCTION: The amidated peptide increases the release of nitric oxid
CC       (NO) and that NO mediates the endothelium-dependent vasodilatation. In
CC       addition, it inhibits efficiently angiotensin I-converting enzyme (ACE)
CC       in rat serum (IC(50)=8.2 uM). 3D-structure modeling of this peptide in
CC       complex with ACE suggests it acts by inhibiting the ACE C-terminal
CC       catalytic site. {ECO:0000269|PubMed:26661890}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26661890}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin gland.
CC       {ECO:0000305|PubMed:26661890}.
CC   -!- PTM: Both amidated and non-amidated peptides have been observed in skin
CC       secretions. {ECO:0000269|PubMed:26661890}.
CC   -!- MASS SPECTROMETRY: Mass=907.37; Method=MALDI; Note=non-amidated.;
CC       Evidence={ECO:0000269|PubMed:26661890};
CC   -!- MASS SPECTROMETRY: Mass=906.36; Method=MALDI; Note=amidated.;
CC       Evidence={ECO:0000269|PubMed:26661890};
CC   -!- SIMILARITY: Belongs to the bradykinin-potentiating peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008217; P:regulation of blood pressure; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Hypotensive agent; Secreted;
KW   Vasoactive.
FT   PEPTIDE         1..8
FT                   /note="Bradykinin-potentiating peptide Brachy"
FT                   /evidence="ECO:0000269|PubMed:26661890"
FT                   /id="PRO_0000455436"
FT   MOD_RES         8
FT                   /note="Proline amide; partial"
FT                   /evidence="ECO:0000269|PubMed:26661890"
SQ   SEQUENCE   8 AA;  907 MW;  6C75A2C327777773 CRC64;
     WPPPKVSP