ID   TAP_MYCTO               Reviewed;         419 AA.
AC   P9WJX8; L0T8U8; P64783; Q11060;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Multidrug efflux pump Tap {ECO:0000250|UniProtKB:O32859};
GN   Name=tap {ECO:0000250|UniProtKB:O32859}; OrderedLocusNames=MT1297;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Efflux pump that contributes to intrinsic antibiotic
CC       resistance. The pump uses the electrochemical gradient as a source of
CC       energy. {ECO:0000250|UniProtKB:O32859}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:O32859}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Drug:H(+)
CC       antiporter-3 (DHA3) (TC 2.A.1.21) family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK45555.1; -; Genomic_DNA.
DR   PIR; B70753; B70753.
DR   RefSeq; WP_003406359.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WJX8; -.
DR   SMR; P9WJX8; -.
DR   EnsemblBacteria; AAK45555; AAK45555; MT1297.
DR   KEGG; mtc:MT1297; -.
DR   PATRIC; fig|83331.31.peg.1400; -.
DR   HOGENOM; CLU_034180_2_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..419
FT                   /note="Multidrug efflux pump Tap"
FT                   /id="PRO_0000427752"
FT   TRANSMEM        7..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        218..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..282
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        289..308
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        313..335
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        348..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        375..397
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   419 AA;  43287 MW;  69EA46BBFF653037 CRC64;
     MRNSNRGPAF LILFATLMAA AGDGVSIVAF PWLVLQREGS AGQASIVASA TMLPLLFATL
     VAGTAVDYFG RRRVSMVADA LSGAAVAGVP LVAWGYGGDA VNVLVLAVLA ALAAAFGPAG
     MTARDSMLPE AAARAGWSLD RINGAYEAIL NLAFIVGPAI GGLMIATVGG ITTMWITATA
     FGLSILAIAA LQLEGAGKPH HTSRPQGLVS GIAEGLRFVW NLRVLRTLGM IDLTVTALYL
     PMESVLFPKY FTDHQQPVQL GWALMAIAGG GLVGALGYAV LAIRVPRRVT MSTAVLTLGL
     ASMVIAFLPP LPVIMVLCAV VGLVYGPIQP IYNYVIQTRA AQHLRGRVVG VMTSLAYAAG
     PLGLLLAGPL TDAAGLHATF LALALPIVCT GLVAIRLPAL RELDLAPQAD IDRPVGSAQ