ID   BPRV_DICNO              Reviewed;         603 AA.
AC   P42779;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Extracellular basic protease;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=bprV; Synonyms=bpr;
OS   Dichelobacter nodosus (Bacteroides nodosus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Cardiobacteriales;
OC   Cardiobacteriaceae; Dichelobacter.
OX   NCBI_TaxID=870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=VCS 1001 / A198;
RX   PubMed=1446666; DOI=10.1111/j.1432-1033.1992.tb17385.x;
RA   Lilley G.G., Stewart D.J., Kortt A.A.;
RT   "Amino acid and DNA sequences of an extracellular basic protease of
RT   Dichelobacter nodosus show that it is a member of the subtilisin family of
RT   proteases.";
RL   Eur. J. Biochem. 210:13-21(1992).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; Z16080; CAA78894.1; -; Genomic_DNA.
DR   PIR; S27055; S27055.
DR   AlphaFoldDB; P42779; -.
DR   SMR; P42779; -.
DR   MEROPS; S08.022; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07496; Peptidases_S8_13; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR002884; P_dom.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR   InterPro; IPR022398; Peptidase_S8_His-AS.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR034176; Peptidases_S8_13.
DR   Pfam; PF01483; P_proprotein; 1.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51829; P_HOMO_B; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Hydrolase; Protease; Secreted;
KW   Serine protease; Signal; Zymogen.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..132
FT                   /id="PRO_0000026998"
FT   CHAIN           133..476
FT                   /note="Extracellular basic protease"
FT                   /id="PRO_0000026999"
FT   PROPEP          477..603
FT                   /id="PRO_0000027000"
FT   DOMAIN          143..468
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   DOMAIN          478..603
FT                   /note="P/Homo B"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01173"
FT   REGION          197..221
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        173
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        237
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        409
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   DISULFID        221..273
FT                   /evidence="ECO:0000269|PubMed:1446666"
FT   DISULFID        315..352
FT                   /evidence="ECO:0000269|PubMed:1446666"
SQ   SEQUENCE   603 AA;  63869 MW;  F3919879E1142E2E CRC64;
     MNLSNISAVK VLTLVVSAAI AGQVCAAESI VNYESANAIS KQPEGSVRFI VKYKDGTPSS
     QGLKTRSTTK VMASGMQVAG FEAQFVRTTG LGAGIFAVPE LKTTKEAHLV MDTIASNPDV
     EFVEVDRLAY PKAAPNDPSY RQQWHYFGNY GVKANKVWDR GFTGQGVVVS VVDTGILDHV
     DLNGNMLPGY DFISSAPNAR DGDQRDNNPA DEGDWFDNWD CGGYPDPRRE KKFSTWHGSH
     VAGTIAAVTN NGVGVAGVAY GAKVIPVRVL GKCGGYDSDI TDGMYWSAGG HIDGVPDNQN
     PAQVVNMSLG GGGGCSQNSQ RMIDKTTNLG ALIVIAAGNE NQDASRTWPS SCNNVLSVGA
     TTPKGKRAPF SNYGARVHLA APGTNILSTI DVGQAGPVRS SYGMKAGTSM AAPHVSGVAA
     LVISAANSIG KTLTPSELSD ILVRTTSRFN GRLDRGLGSG IVDANAAVNA VLGDQNRAQP
     RPPVNQPINS GNKVYRSDRR VAIRDLRSVT SGIRVNDQAR VGSANITLTL DIRYGDRSQL
     AVELIAPSGR VYPIYHDGKR QPNIVGPATF SVKNERLQGT WTLKVTDKAR GVTGSIDSWS
     LTF