ID   TAS1_PSEAB              Reviewed;         439 AA.
AC   A0A0H2ZJS4;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 14.
DE   RecName: Full=(p)ppApp synthetase toxin Tas1;
GN   Name=tas1; OrderedLocusNames=PA14_01140;
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND DISRUPTION PHENOTYPE.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=31695193; DOI=10.1038/s41586-019-1735-9;
RA   Ahmad S., Wang B., Walker M.D., Tran H.R., Stogios P.J., Savchenko A.,
RA   Grant R.A., McArthur A.G., Laub M.T., Whitney J.C.;
RT   "An interbacterial toxin inhibits target cell growth by synthesizing
RT   (p)ppApp.";
RL   Nature 575:674-678(2019).
CC   -!- FUNCTION: Type VI secretion exported toxin that pyrophosphorylates
CC       adenosine nucleotides to produce (p)ppApp. Thereby, depletes cellular
CC       ADP and ATP to dysregulate central metabolism in competitor cells.
CC       {ECO:0000269|PubMed:31695193}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + ATP = adenosine 3'-diphosphate,5'-phosphate + AMP +
CC         H(+); Xref=Rhea:RHEA:62336, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:145541, ChEBI:CHEBI:456215;
CC         Evidence={ECO:0000269|PubMed:31695193};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ADP + ATP = adenosine 3'-diphosphate,5'-diphosphate + AMP;
CC         Xref=Rhea:RHEA:62332, ChEBI:CHEBI:30616, ChEBI:CHEBI:145676,
CC         ChEBI:CHEBI:456215, ChEBI:CHEBI:456216;
CC         Evidence={ECO:0000269|PubMed:31695193};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 ATP = adenosine 3'-diphosphate,5'-triphosphate + AMP;
CC         Xref=Rhea:RHEA:59820, ChEBI:CHEBI:30616, ChEBI:CHEBI:145540,
CC         ChEBI:CHEBI:456215; Evidence={ECO:0000269|PubMed:31695193};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:31695193}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant in PA14 strain displays an
CC       approximately 40-fold decrease in competitive index against PAO1
CC       strain. {ECO:0000269|PubMed:31695193}.
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DR   EMBL; CP000438; ABJ15050.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0H2ZJS4; -.
DR   SMR; A0A0H2ZJS4; -.
DR   EnsemblBacteria; ABJ15050; ABJ15050; PA14_01140.
DR   KEGG; pau:PA14_01140; -.
DR   HOGENOM; CLU_594279_0_0_6; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008727; PAAR_motif.
DR   Pfam; PF05488; PAAR_motif; 1.
PE   1: Evidence at protein level;
KW   Secreted.
FT   CHAIN           1..439
FT                   /note="(p)ppApp synthetase toxin Tas1"
FT                   /id="PRO_0000449568"
SQ   SEQUENCE   439 AA;  45115 MW;  D1CE377322D5671E CRC64;
     MVAGAVAGAL IGAAVVAATA ATGGLAAVIL AGSIAAGGLS MFQIVKGLTT IFELPEPTTG
     VLIRGSFNVY VNSRNAMRAG DDVSATCSGL PLNHPLWPFP VLIAEGSATV YINGKPAARL
     QSKMVCGAHI KTGSQNTFIG GPTERVAFVL DLEEWLHTGL EALGLAALAG GLLLAAMAGV
     AALVGVVAIG GLMMGGMALL GDLGDRLGPG YRDLFQGVAG MALLGFGPKM ARLGNAPKGA
     PKTQVPKGFE KVYGKAPAAK AEIDAVADGL AAKHGGRVAK APIKSRERAM QKINNDYKGD
     PTKIKDLARN TIIVEGDKVN TVAAELANRG AKVKVIDGNA DPLGYSGVNS TMNTKAGIPG
     EIQVNSPEMI YAKESEDMAR ILLGNDTYDA VAAKAGVPGG QGHKYYEDWR VLDPKSPEAQ
     AIAEKSRAYY DAVRKGNGN