TATC_ARATH
ID TATC_ARATH Reviewed; 340 AA.
AC Q9SJV5; Q9XFI4;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Sec-independent protein translocase protein TATC, chloroplastic;
DE AltName: Full=Protein ALBINO AND PALE GREEN 2;
DE AltName: Full=Protein TWIN-ARGININE TRANSLOCATION C;
DE AltName: Full=Protein UNFERTILIZED EMBRYO SAC 3;
DE Flags: Precursor;
GN Name=TATC; Synonyms=APG2, UNE3; OrderedLocusNames=At2g01110;
GN ORFNames=F23H14.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=11526245; DOI=10.1073/pnas.181304598;
RA Motohashi R., Nagata N., Ito T., Takahashi S., Hobo T., Yoshida S.,
RA Shinozaki K.;
RT "An essential role of a TatC homologue of a Delta pH- dependent protein
RT transporter in thylakoid membrane formation during chloroplast development
RT in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:10499-10504(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Summer E.J., McCaffery M.W., Cline K.;
RT "An Arabidopsis homolog of TatC/YCF43.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP SUBUNIT.
RX PubMed=18930082; DOI=10.1016/j.bbamcr.2008.09.006;
RA Jakob M., Kaiser S., Gutensohn M., Hanner P., Kloesgen R.B.;
RT "Tat subunit stoichiometry in Arabidopsis thaliana challenges the proposed
RT function of TatA as the translocation pore.";
RL Biochim. Biophys. Acta 1793:388-394(2009).
CC -!- FUNCTION: Part of the twin-arginine translocation (Tat) system that
CC transports large folded proteins containing a characteristic twin-
CC arginine motif in their signal peptide across the thylakoid membrane.
CC Involved in delta pH-dependent protein transport required for
CC chloroplast development, especially thylakoid membrane formation. TATC
CC and TATB mediate precursor recognition, whereas TATA facilitates
CC translocation. {ECO:0000269|PubMed:11526245}.
CC -!- SUBUNIT: In thylakoid membranes, TATC and TATB form a large receptor
CC complex, containing about eight TATC-TATB pairs, which binds the
CC precursor protein. Twin arginine signal peptide promotes pH-triggered
CC docking of TATA oligomers to TATC-TATB receptor complex, inducing a
CC conformational switch of TATA that results in activation of the
CC translocase. TATA dissociates from TATC-TATB upon completion of
CC translocation. {ECO:0000269|PubMed:18930082}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305|PubMed:11526245}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:11526245}. Note=The N-terminus is located in the
CC stroma. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Seedling lethality when homozygous. When grown on
CC agar medium, mutant seedlings have an albino phenotype and contain
CC plastid lacking internal membrane structures.
CC {ECO:0000269|PubMed:11526245}.
CC -!- MISCELLANEOUS: According to PubMed:18930082, TATA is detectable only in
CC minor amounts in Arabidopsis chloroplasts.
CC -!- SIMILARITY: Belongs to the TatC family. {ECO:0000305}.
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DR EMBL; AB054096; BAB62074.1; -; mRNA.
DR EMBL; AF145045; AAD33946.1; -; mRNA.
DR EMBL; AC006837; AAF18659.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC05402.1; -; Genomic_DNA.
DR EMBL; AF360172; AAK25882.1; -; mRNA.
DR EMBL; AY056347; AAL07196.1; -; mRNA.
DR EMBL; AY084784; AAM61351.1; -; mRNA.
DR PIR; T44583; T44583.
DR RefSeq; NP_565252.1; NM_126173.4.
DR AlphaFoldDB; Q9SJV5; -.
DR SMR; Q9SJV5; -.
DR STRING; 3702.AT2G01110.1; -.
DR TCDB; 2.A.64.2.1; the twin arginine targeting (tat) family.
DR PaxDb; Q9SJV5; -.
DR PRIDE; Q9SJV5; -.
DR ProteomicsDB; 234182; -.
DR EnsemblPlants; AT2G01110.1; AT2G01110.1; AT2G01110.
DR GeneID; 814640; -.
DR Gramene; AT2G01110.1; AT2G01110.1; AT2G01110.
DR KEGG; ath:AT2G01110; -.
DR Araport; AT2G01110; -.
DR TAIR; locus:2045750; AT2G01110.
DR eggNOG; ENOG502QVCB; Eukaryota.
DR HOGENOM; CLU_031942_2_0_1; -.
DR InParanoid; Q9SJV5; -.
DR OMA; EMSFLDH; -.
DR OrthoDB; 1204533at2759; -.
DR PhylomeDB; Q9SJV5; -.
DR PRO; PR:Q9SJV5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJV5; baseline and differential.
DR Genevisible; Q9SJV5; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR GO; GO:0031361; C:integral component of thylakoid membrane; IDA:TAIR.
DR GO; GO:0043235; C:receptor complex; IDA:TAIR.
DR GO; GO:0033281; C:TAT protein transport complex; IDA:UniProtKB.
DR GO; GO:0009977; F:proton motive force dependent protein transmembrane transporter activity; ISS:TAIR.
DR GO; GO:0009567; P:double fertilization forming a zygote and endosperm; IMP:TAIR.
DR GO; GO:0065002; P:intracellular protein transmembrane transport; IBA:GO_Central.
DR GO; GO:0043953; P:protein transport by the Tat complex; IBA:GO_Central.
DR GO; GO:0010027; P:thylakoid membrane organization; IMP:TAIR.
DR HAMAP; MF_00902; TatC; 1.
DR InterPro; IPR019820; Sec-indep_translocase_CS.
DR InterPro; IPR002033; TatC.
DR PANTHER; PTHR30371; PTHR30371; 1.
DR Pfam; PF00902; TatC; 1.
DR PRINTS; PR01840; TATCFAMILY.
DR TIGRFAMs; TIGR00945; tatC; 1.
DR PROSITE; PS01218; TATC; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Protein transport; Reference proteome;
KW Thylakoid; Transit peptide; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..28
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 29..60
FT /note="Thylakoid"
FT /evidence="ECO:0000305"
FT CHAIN 61..340
FT /note="Sec-independent protein translocase protein TATC,
FT chloroplastic"
FT /id="PRO_0000419910"
FT TOPO_DOM 61..127
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..179
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..209
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 231..257
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..289
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 311..314
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 336..340
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT REGION 57..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 340 AA; 37367 MW; 1429C265BFEDB281 CRC64;
MSSTSTSSAL IHHFRLTTRN LGSPTKQRCP YAVTFCNSWR EAGLRYSVTQ RRSKGFGPVS
ALNDDDSPTE TTPGVGSAVE DRPPDSSEDR SSSVYEFLYP RKEELPDDKE MTIFDHLEEL
RERIFVSVLA VGAAILGCFA FSKDLIVFLE APVKTQGVRF LQLAPGEFFF TTLKVSGYCG
LLLGSPVILY EIIAFVLPGL TRAERRFLGP IVFGSSLLFY AGLAFSYWVL TPAALNFFVN
YAEGVVESLW SIDQYFEFVL VLMFSTGLSF QVPVIQLLLG QVGVVSGDQM LSIWRYVVVG
AVVAAAVVTP STDPVTQMLL ATPLLGLYLG GAWMVKLTGR
